Human Hemoglobin subunit alpha ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Hemoglobin subunit alpha with a sensitivity of 131 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Validated on a number of sample types including cerebrospinal fluid (CSF).