Human Sorbitol Dehydrogenase ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Sorbitol Dehydrogenase with a sensitivity of 60 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader.