Human Alpha-1-Microglobulin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Alpha-1-Microglobulin with a sensitivity of 725 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader.