ALiCE® - Linearly scalable eukaryotic cell-free protein expression. Scalable protein expression with ALiCE® works as effectively for assessment of future development as it does in the exploration phase. The same simple protocol applies across all scales. Add the DNA sequence and let ALiCE® make your protein.
- For production of high-value proteins in mg-scale:
- DNA for cytosolic expression
- DNA for microsomal expression
- Protocol for scaled reaction up to 100 ml
ALiCE® for Scale-Up is a powerful protein production solution, optimised to support milligram scale protein expression for in-depth analysis and characterisation studies.
Based on the same technology as our popular ALiCE® for Research Kits, ALiCE® for Scale-Up consistently produces high protein yields of over 2 mg/ml (eYFP standard). Originating from N. tabacum c.v. BY-2 cells, this eukaryotic cell-free expression system, contains native microsomes derived from endoplasmic reticulum and Golgi apparatus that allow easy production of proteins with membrane-spanning domains or eukaryotic post-translational modifications, including di-sulfide bonds and N-glycosylation. With everything required for protein expression included in the lysate (amino acids, energy supply, co-factors, etc.), the reaction is initiated by simply adding DNA.
ALiCE® for Scale-Up is provided together with two vectors, pALiCE01 and pALiCE02, which enable the user to produce proteins via the cytosolic or microsomal pathways for optimal flexibility. The pALiCE01 and pALiCE02 vectors are also hosted at 3rd party vendors (Twist and IDT), allowing simple synthesis direct of ready-to-use template DNA.
Note: ALiCE® for Scale-Up is also compatible with linear DNA formats. Details for optimised expression available upon request. For larger-scale protein characterisation and development projects, ALiCE® for Scale-Up provides the flexibility and scalability required to support your protein discovery objectives.
Storage Conditions: They should be transferred directly to a –80 °C freezer upon delivery.