Derived from KG-1, however KG1a is unresponsive to colony stimulating factor in soft agar culture and does not express the Ia-like antigen.

KG1a is resistant to phorbol-diester induced macrophage differentiation and cell proliferation. KG1a cells are morphologically, cytochemically and functionally less mature than the parental KG-1. Expresses HLA A30,31; B35; CW4.

DNA profile: STR-PCR data: Amelogenin: X,Y, CSF1PO: 7, D13S317: 11,12, D16S539: 10,11, D5S818: 13, D7S820: 8,10, THO1: 7,8, TPOX: 7,9, vWA: 14,19.

Culture medium: IDMEM + 2 mM Glutamine + 20% Foetal Bovine Serum (FBS).
Subculture routine: Maintain cultures between 5×100000 to 1000000 cells/ml. Do not culture below 100000 cells/ml; 5% CO₂; 37 °C.

Karyotype: 2n = 46, pseudodiploid
Morphology: Lymphoblast
Technique: Cell culture
Relevant disease: Cancer