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Human EZH2 ELISA Kit
Human EZH2 ELISA Kit
  ANTIA78253-96
 :  Antibodies.com
New Product
 :  
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Human EZH2 ELISA Kit
  ANTIA78253-96
 :  Antibodies.com
New Product
 :  

 

  • Pk:
    96 Tests
  • Assay duration:
    Multiple steps
  • Assay Type:
    Sandwich
  • Format:
    Pre-coated
  • Primary antibody reactivity:
    Human
  • Target protein:
    EZH2
  • Description:
    Human EZH2 ELISA kit
  • Sample type:
    Serum, plasma, tissue homogenates and other biological fluids
  • Cross reactivity:
    Human EZH2 ELISA kit exhibits high specificity and excellent specificity for the detection of human EZH2. No significant cross-reactivity or interference between EZH2 and analogues was observed.
  • Detection method:
    Colorimetric
  • Time to Results:
    4 h 30 min
  • Assay Principle:
    Quantitative
  • Shelf life:
    Store for 6 months at 4 °C
  • Detection range:
    31,25 - 2000 pg/ml
  • Storage temperature:
    4 °C
  • Sample volume:
    100 μl
  • Sensitivity:
    18,75 pg/ml
  • Regulatory status:
    RUO

 

 

Human EZH2 ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human EZH2 in serum, plasma, tissue homogenates, and other biological fluids.

  • Ready to use ELISA kit
  • Detection range: 31,25 to 2000 pg/ml
  • Sensitivity: 18,75 pg/ml
  • Sample type: Serum, plasma, tissue homogenates, and other biological fluids
  • Assay Precision: Intra assay: CV <8%, Inter assay: CV <10%

Human EZH2 ELISA kit (A78253) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human EZH2 in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for EZH2 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the EZH2 present in each sample is bound to the wells by the immobilised antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-EZH2 Antibody, which binds the captured EZH2 present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualise the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of EZH2 captured in each well. The concentration of EZH2 can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.

 : This is a ready to use ELISA kit that contains: Pre-coated 96 well microplate, lyophilised standard, sample dilution buffer, biotinylated detection antibody (100X), antibody dilution buffer, HRP-Streptavidin Conjugate (SABC) (100X), SABC dilution buffer, TMB substrate, stop solution, wash buffer (25X), plate sealers, foil pouch, and protocol manual.