GelCode blue safe protein stain is a fast and sensitive coomassie-dye reagent that provides one-step ultrasensitive detection of proteins in polyacrylamide gels and is formulated to avoid hazardous shipping.

• Sensitivity detect down to 9 ng of protein per band in 15 minutes
• Safety non-corrosive to skin, non-flammable and safe to ship and store until activated
• Additional reagents water washes only, no acid-fixative or methanol destain solutions required
• Compatibility suitable for staining 1D and 2D polyacrylamide gels and nitrocellulose and PVDF membranes (multiple protocols provided in the instructions)
• Applications qualitative visualisation, quantitative densitometry, and downstream excision and analysis by mass spectrometry
• Easy to use add and dissolve activator crystals before first use, then simply add reagent to stain gels
• Stable store non-activated or activated stain at room temperature for up to one year

For proper protein staining, the activator crystals must be dissolved in the staining reagent before use. Add the entire contents of the activator crystals bottle into the GelCode blue safe protein stain bottle. Dissolve the crystals by mixing end-over-end for 1 minute, and then allow it to remain at room temperature for 5 minutes before use. Store the activated stain at room temperature.

Detection location: In-blot detection, In-gel detection
Detection method: Colorimetric
Label or dye: Coomassie
Target molecule: Protein