Specifikace
- Antibody type:Primary
- Antigen name:RNA polymerase 2
- Clonality:Polyclonal
- Conjugation:Alexa Fluor® 750
- Flow cytometry:Yes
- Host:Rabbit
- ImmunoChemistry:Yes
- ImmunoFluorescence:Yes
- Isotype:IgG
- Reactivity:Mouse
- Western blot:Yes
- Epitope:101-200/1970
- Form:Liquid
- Gene ID:5430
- Antigen synonyms:DNA directed RNA polymerase II A|DNA-directed RNA polymerase II largest subunit|RPB1_HUMAN|DNA-directed RNA polymerase II subunit A|POLR2|POLR2A
- Modification:Unmodified
- Storage buffer:Aqueous buffered solution containing 0,01 M TBS (pH 7,4) with 1% BSA, 0,03% Proclin300 and 50% Glycerol
- Molecular weight:217 kDa
- Storage temperature:Store at ‒20 °C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
- Concentration:1 µg/µl
- Shipping temperature:4 °C
- Immunogen:KLH conjugated synthetic peptide derived from human Pol II/RNA polymerase II
- Tested applications:ICC
- Purification:Purified by Protein A
- Pack type:Vial
- Pk:100 µl
Specifikace
O tomto produktu
RNA polymerase II (Pol II) is an enzyme that is composed of twelve subunits and is responsible for the transcription of protein-coding genes. Transcription initiation requires Pol II-mediated recruitment of transcription machinery to a target promoter, thereby allowing transcription to begin. The largest subunit of Pol II (referred to as RPB1 or RPB205) is a 1,840 amino acid protein that contains one C2H2-type zinc finger and a C-terminal domain comprised of several heptapeptide repeats. Although Pol II function requires the cooperation of all twelve subunits, the largest subunit conveys Pol II catalytic activity and, together with the second largest subunit, forms the active center of the Pol II enzyme. Additionally, the large subunit participates in forming the DNA-binding domain of Pol II, a groove that is necessary for transcription of the DNA template. Without proper function of the large subunit, mRNA synthesis and subsequent transcription elongation cannot occur.
Type: Primary
Antigen: RNA polymerase 2
Clonality: Polyclonal
Clone:
Conjugation: ALEXA FLUOR® 750
Public Immunogen Range: 101-200/1970
Host: Rabbit
Isotype: IgG
Reactivity: Mouse