Reusable, Endotoxin free Protein A conjugated Agarose beads with ≥15 mg/ml binding capacity.

• APPLICATIONS
• Purification of monoclonal and polyclonal antibodies from culture media, serum, ascites fluid or hybridoma supernatants
• Isolation of antibody/antigen complexes in immunoprecipitation experiments, since only the Fc region is involved in antibody binding and the Fab region is available for binding antigen

Endo toxin free Protein A-Agarose beads are prepared by covalently coupling recombinant Protein A to 6% cross-linked Agarose beads under proprietary endotoxin-free conditions. BioVision’s Protein A (Cat. No. 6500, Cat. No. 6500B) is a genetically engineered protein containing five IgG-binding regions of native Protein A. The cell wall binding region, albumin binding region and other non-specific regions have been eliminated from the recombinant Protein A to ensure the maximum specific IgG binding. Protein A-Agarose beads display high chemical and physical stability as well as high flow rate, hydrophilicity and high gel strength. It can be used for IgG purification and immunoprecipitation. Protein A-Agarose beads are prepared by covalently coupling recombinant Protein A to 6% cross-linked Agarose beads. The coupling technique is optimized to give a higher binding capacity for IgG and minimum leaching of recombinant Protein A. The IgG binding capacity of Protein A-Agarose is ≥15 mg human or rabbit IgG per ml of settled beads. Our Endotoxin-free Protein A-Agarose also shows ability of reducing/removing certain amount of endotoxin from serum or ascites samples.

All products are FOR RESEARCH USE ONLY.   PRODUCTS ARE NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES.  Customer is granted a limited use non-exclusive licence for its own internal research.  Any control manufacturer supplies with a Product is limited to use only to validate the relevant Product.  By using the Product, Customers agree not to attempt to reverse engineer or otherwise perform any compositional, structural, functional or other analyses directed to learning the methodology, formulae, sequences, processes, make-up or production of any of the Products or portions thereof.