Dulbecco's Modified Eagle's Medium (DMEM) with L-Glutamine with 4,5 g/L glucose without sodium bicarbonate is the most widely used modification of Eagle's Basal Medium (BME).

• Ideally for supporting and maintaining a vast range of mammalian cell types
• It contains four times greater concentration of amino acids, vitamins and supplementary components

The original formulation calls for 1000 mg/L of glucose, which was first employed to support the polyoma virus in primary and secondary embryonic mouse cultures. For optimal culturing of other cell types, a modification of 4500 mg/L glucose is available.