Mouse Recombinant TNF-A (from E. coli)

Tumor necrosis factor alpha (TNF-α) is an inflammatory cytokine secreted by macrophages, monocytes, neutrophils, T cells, and NK-cells following stimulation by bacterial lipopolysaccharide (LPS). TNF-α signal activation occurs through two receptors, TNFR1 and TNFR2. TNFR1 is expressed on most cell types, unlike TNFR2, which is expressed mainly on immune cells. TNF-α functions to stimulate phagocytosis in macrophages, chemoattract neutrophils, increase insulin resistance, and induce fever.

High quality
Low endotoxin
Affordable

: This product is for research use only and is not for use in humans.

Spojení:
Unconjugated
Proteinový podtyp:
Cytokine
Typ proteinu/peptidu:
Recombinant
Zdroj:
E. coli
Druhy:
Mouse
Podmínky uskladnění:
–20 °C
Obsah endotoxinů:
Endotoxin LAL (EU/µg) ≤0,1
Biologická aktivita:
Bioactivity Assay: Cytolysis of mouse L929 cells in the presence of Actinomycin D, ED₅₀ ≤0,1 ng/ml, Bioactivity ≥1,0×10⁷ units/mg
Identifikační číslo genu:
P06804
Pokyny pro rekonstituce:
Sterile water at 0,1 mg/ml
Bez obsahu endotoxinů:
N
Bez nosiče:
Y
Bez obsahu proteázy:
N
Bez obsahu živočichů:
Y
Proteinová synonyma:
Cytotoxin|DIF|Necrosin|Cachexin|TNFSF2|TNF|Cachectin
Název proteinu/peptidu:
TNF-A (AF)
Čistota:
≥95%
Molekulární hmotnost:
17,4 kDa
Sekvence:
MLRSSSQNSS DKPVAHVVAN HQVEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLVY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VNLLSAVKSP CPKDTPEGAE LKPWYEPIYL GGVFQLEKGD QLSAEVNLPK YLDFAESGQV YFGVIAL
Úroveň endotoxinů:
Low
Formulace:
10 mM sodium phosphate, 50 mM sodium chloride, pH 7,5
Bez nukleáz:
N
Třída:
RUO
Transportní teplota:
RT
Testované aplikace:
Bioassay