Vitamin D ELISA kit
About this item
Sensitive ELISA for vitamin D quantification in plasma or serum.
- Measure vitamin D in plasma or serum
- Detect the metabolically stable forms 25(OH)D₃ and 25(OH)D₂
- Assay 24 samples in triplicate or 36 samples in duplicate
- Measure vitamin D levels down to 0,5 ng/ml
- Read: Colorimetric at 405 to 420 nm
The primary synthesis of functional vitamin D begins in the skin, where a cholesterol by-product acted upon by UV light forms vitamin D₃. Vitamin D₃ is carried through the bloodstream, attached to the vitamin D binding protein, to the liver where it is converted to 25-hydroxy vitamin D₃ (25(OH)D₃). This product is then transported to the kidney where it is further converted to 1,25-dihydroxycholecalciferol (1,25(OH)₂D₃), the biologically active form. Active 1,25(OH)₂D₃ is very short-lived and is rapidly metabolized to its deactivated forms.
Vitamin D Immunoassay, accurate Vitamin D measurement in plasma or serum. Vitamin D ELISA Kit is a competitive assay that can be used for Vitamin D quantification in plasma or serum. Because of the short half-life of the biologically active 1,25(OH)D form, this Vitamin D assay primarily detects the more metabolically stable forms, 25(OH)D₃ and 25(OH)D₂.
Accurate detection of these forms requires that they be displaced from the vitamin D binding protein prior to measurement. A simple, acetone-based purification protocol is included as the initial procedure of the Vitamin D assay kit for this purpose.
Packaging: Kit contains: Antibody, tracer, standard, ELISA and wash buffer concentrate, polysorbate 20, plate and cover, Ellman's reagent, tracer and antiserum dye.
4 Options Available Below
Product Details & Documents
Sensitive ELISA for vitamin D quantification in plasma or serum.
- Measure vitamin D in plasma or serum
- Detect the metabolically stable forms 25(OH)D₃ and 25(OH)D₂
- Assay 24 samples in triplicate or 36 samples in duplicate
- Measure vitamin D levels down to 0,5 ng/ml
- Read: Colorimetric at 405 to 420 nm
The primary synthesis of functional vitamin D begins in the skin, where a cholesterol by-product acted upon by UV light forms vitamin D₃. Vitamin D₃ is carried through the bloodstream, attached to the vitamin D binding protein, to the liver where it is converted to 25-hydroxy vitamin D₃ (25(OH)D₃). This product is then transported to the kidney where it is further converted to 1,25-dihydroxycholecalciferol (1,25(OH)₂D₃), the biologically active form. Active 1,25(OH)₂D₃ is very short-lived and is rapidly metabolized to its deactivated forms.
Vitamin D Immunoassay, accurate Vitamin D measurement in plasma or serum. Vitamin D ELISA Kit is a competitive assay that can be used for Vitamin D quantification in plasma or serum. Because of the short half-life of the biologically active 1,25(OH)D form, this Vitamin D assay primarily detects the more metabolically stable forms, 25(OH)D₃ and 25(OH)D₂.
Accurate detection of these forms requires that they be displaced from the vitamin D binding protein prior to measurement. A simple, acetone-based purification protocol is included as the initial procedure of the Vitamin D assay kit for this purpose.
Packaging: Kit contains: Antibody, tracer, standard, ELISA and wash buffer concentrate, polysorbate 20, plate and cover, Ellman's reagent, tracer and antiserum dye.
Documents
TDS Vitamin D ELISA Kit
Datasheet
Specifications for Product Family
Specifications
Specifications
- Catalog No:
- CAYM501050-96S
- CAYM501050-96
- CAYM501050-480S
- CAYM501050-480
- Sensitivity:
- 0,5 ng/ml (80% B/B₀)
- 0,5 ng/ml (80% B/B₀)
- 0,5 ng/ml (80% B/B₀)
- 0,5 ng/ml (80% B/B₀)
- Detection range:
- 0,19 - 25 ng/ml
- 0,19 - 25 ng/ml
- 0,19 - 25 ng/ml
- 0,19 - 25 ng/ml
- Mid point:
- 1 - 4 ng/ml (50% B/B₀)
- 1 - 4 ng/ml (50% B/B₀)
- 1 - 4 ng/ml (50% B/B₀)
- 1 - 4 ng/ml (50% B/B₀)
- Developmental time:
- 60 - 90 min
- 60 - 90 min
- 60 - 90 min
- 60 - 90 min
- Incubation time:
- 18 hr
- 18 hr
- 18 hr
- 18 hr
- Storage temperature:
- –20 °C
- –20 °C
- –20 °C
- –20 °C
Specifications
Product Family Options
Product Information
- PkAvailabilityPrice
- CAYM501050-96SVitamin D ELISA Kit, 96 solid wellsSpecifications:
More
Specifications
Cat. No.CAYM501050-96SSensitivity0,5 ng/ml (80% B/B₀)Detection range0,19 - 25 ng/mlMid point1 - 4 ng/ml (50% B/B₀)Developmental time60 - 90 minIncubation time18 hrStorage temperature–20 °C - CAYM501050-96Vitamin D ELISA Kit, 96 strip wellsSpecifications:
More
Specifications
Cat. No.CAYM501050-96Sensitivity0,5 ng/ml (80% B/B₀)Detection range0,19 - 25 ng/mlMid point1 - 4 ng/ml (50% B/B₀)Developmental time60 - 90 minIncubation time18 hrStorage temperature–20 °C - CAYM501050-480SVitamin D ELISA Kit, 480 solid wellsSpecifications:
More
Specifications
Cat. No.CAYM501050-480SSensitivity0,5 ng/ml (80% B/B₀)Detection range0,19 - 25 ng/mlMid point1 - 4 ng/ml (50% B/B₀)Developmental time60 - 90 minIncubation time18 hrStorage temperature–20 °C - CAYM501050-480Vitamin D ELISA Kit, 480 strip wellsSpecifications:
More
Specifications
Cat. No.CAYM501050-480Sensitivity0,5 ng/ml (80% B/B₀)Detection range0,19 - 25 ng/mlMid point1 - 4 ng/ml (50% B/B₀)Developmental time60 - 90 minIncubation time18 hrStorage temperature–20 °C
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