Rat Recombinant TNFalpha (from E. coli)

About this item

Tumor necrosis factor alpha (TNF-α) is an inflammatory cytokine secreted by macrophages, monocytes, neutrophils, T cells, and NK-cells following stimulation by bacterial lipopolysaccharide (LPS). TNF-α signal activation occurs through two receptors, TNFR1 and TNFR2. TNFR1 is expressed on most cell types, unlike TNFR2, which is expressed mainly on immune cells. TNF-α functions to stimulate phagocytosis in macrophages, chemoattract neutrophils, increase insulin resistance, and induce fever.

High Quality
Low Endotoxin
Affordable

A COA is supplied with each product shipped detailing purity, formulation, biological activity, endotoxin levels, reconstitution instructions and recommended storage conditions. This product is for research use only and is not for use in humans.

Caution: For research use only.

Specifications

Conjugation:
Unconjugated
Protein function:
Cytokine
Protein/peptide type:
Recombinant
Source:
E. coli
Species:
Rat
Storage conditions:
–20 °C
Endotoxin content:
Endotoxin LAL (EU/µg) ≤1
Biological activity:
Bioactivity assay: Cytolysis of mouse L929 cells in the presence of Actinomycin D, ED50 ≤0,05 ng/ml, Bioactivity ≥2,0×10⁷ units/mg
Gene ID:
P16599
Reconstitution instructions:
Sterile water at 0.1 mg/ml
Endotoxin-free:
N
Carrier-free:
Y
Protease-free:
N
Animal-free:
N
Protein synonyms:
Cytotoxin|DIF|Necrosin|Cachexin|TNFSF2|TNF|Cachectin
Protein/peptide name:
TNFalpha
Purity:
≥95%
Molecular weight:
17,3 kDa
Sequence:
MLRSSSQNSS DKPVAHVVAN HQAEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLIY SQVLFKGQGC PDYVLLTHTV SRFATSYQEK VSLLSAIKSP CPKDTPEGAE LKPWYEPMYL GGVSQLEKGD LLSAEVNLPK YLDITESGQV YFGVIAL
Endotoxin level:
Low
Formulation:
10 mM sodium phosphate, 50 mM sodium chloride, pH 7,5
Nuclease-free:
N
Grade:
RUO
Shipping temperature:
RT
Tested applications:
Bioassay