Human Recombinant PDGF-AA (from E. coli)

Supplier: Shenandoah Biotechnology

Certificates

Supplier: Shenandoah Biotechnology

About this item

Platelet-derived growth factor (PDGF) is an important regulator of cell growth, proliferation, and angiogenesis. PDGF synthesis is induced by IL-1, IL-6, TNF-α, TGF-β and EGF signaling. PDGF functions as a mitogenic growth hormone on cells of mesenchymal lineage, such as smooth muscle and glial cells.

High quality
Low endotoxin
Affordable

PDGF is also stored in the alpha-granules of platelets and is released upon adherence to traumatized tissues. PDGF is a dimeric glycoprotein formed by two A chains (AA), two B chains (BB), or as a heterodimer with an A and a B chain (AB). The PDGF dimer binds the cell surface receptor tyrosine kinases PDGFR-α and PDGFR-β.

These research grade recombinant proteins are typically used as cytokines, growth factors and chemokines in cell based assays and cell culture.

Delivery: A certificate of analysis (COA) is supplied with each product shipped detailing purity, formulation, biological activity, endotoxin levels, reconstitution instructions and recommended storage conditions.

Caution: For research use only.

Specifications

Conjugation:
Unconjugated
Protein function:
Growth factor
Protein/peptide type:
Recombinant
Source:
E. coli
Species:
Human
Storage conditions:
−20 °C
Endotoxin content:
Endotoxin LAL (EU/µg) ≤0.1
Biological activity:
Bioactivity assay: NR6R-3T3 cell proliferation, ED50 ≤50 ng/ml, bioactivity ≥2,0×10⁴ units/mg
Gene ID:
P04085
Reconstitution instructions:
Sterile water at 0,1 mg/ml
Endotoxin-free:
N
Carrier-free:
Y
Protease-free:
N
Animal-free:
N
Protein synonyms:
ODGF|GDGF
UniProtKB:
P04085
Protein/peptide name:
PDGF-AA
Purity:
≥95%
Molecular weight:
14,4/28,9 (kDa)
Sequence:
MSIEEAVPAV CKTRTVIYEI PRSQVDPTSA NFLIWPPCVE VKRCTGCCNT SSVKCQPSRV HHRSVKVAKV EYVRKKPKLK EVQVRLEEHL ECACATTSLN PDYREEDTGR PRESGKKRKR KRLKPT
Endotoxin level:
Low
Formulation:
0,1% Trifluoroacetic Acid (TFA)
Nuclease-free:
N
Grade:
RUO
Shipping temperature:
RT
Tested applications:
Bioassay