- Pk:1 KIT
- Assay Type:Competitive
- Host:
- Primary antibody reactivity:
- Target protein:cGMP
- Description:cGMP complete ELISA kit
- Seq:02
- Environmentally Preferable:
- Sensitivity:Assay Buffer : 0,42 pmol/ml (non-acetylated) 0,043 pmol/ml (acetylated) HCl: 0,604 pmol/ml (non-acetylated) 0,059 pmol/ml (acetylated) (range 0,8 - 500 pmol/ml (non-acetylated); 0,08 - 50 pmol/ml (acetylated))
Most sensitive (0,043 pmol/ml) and complete colorimetric kit for intra- and extracellular cGMP quantification.
For the quantitative determination of cGMP in cell lysates, culture supernatants, plasma, serum, saliva, tissue, and urine from any species.
This is a colorimetric competitive immunoassay kit for the quantitative determination of extracellular cGMP diluted in buffer or intracellular cGMP in cells or tissues lysed in 0.1 M HCl. 0.1M HCl is used to stop endogenous phosphodiesterase activity and stabilize the released cyclic GMP. The 0.1M HCl treated samples are then analyzed directly in a microtiter plate without extraction, drying and reconstitution. Sensitivity is increased by acetylation (reagents included). Absorbance is read at 405 nm.The ready-to-use liquid color-coded reagents provided reduce error.