Specifications

• Protein/peptide type:
  Recombinant
• Source:
  P. pastoris
• Species:
  Human
• Storage conditions:
  Lyophilized Granulocyte Macrophage Colony Stimulating Factor although stable at room temperature for weeks, should be stored desiccated below −18 °C. Upon reconstitution GMCSF should be stored at 4 °C between 2-7 days and for future use below −18 °C. For long term storage it is recommended to add carrier protein (0. 1% HSA or BSA). Please prevent freeze/thaw cycles.
• Biological activity:
  The ED50 as determined by the dose-dependant stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) is 0.183 ng/ml, corresponding to Specific Activity of 5,000,000 IU/mg.
• Protein synonyms:
  CSF-2
• Protein/peptide name:
  GM CSF Pichia
• Purity:
  Greater than 97.0% as determined by1. Analysis by RP-HPLC.2. Analysis by SDS-PAGE.
• Solubility:
  It is recommended to reconstitute the lyophilized Granulocyte Macrophage Colony Stimulating Factor in sterile H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
• Sequence:
  The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Ala-Arg-Ser.
• Formulation:
  The protein was lyophilized from concentrated (1mg/ml) solution containing 10mM phosphate buffer pH 7.0, 40 mg mannitol and 10 mg sucrose.
• Tested applications:
  SDS-PAGE