Pierce™ Immobilised Papain consists of a cysteine-endopeptidase that has been immobilised onto beaded agarose resin to enable generation and purification of Fab fragments from antibodies.

• Papain can also be used to generate F(ab')2 fragments
• Eliminates autolysis and contamination of a sample with the protease
• Allows control of the digestion by removing the papain or controlling the flow of sample over an immobilized papain column
• Immobilized papain is more stable against heat-induced denaturation, resulting in longer maintenance of activity

Papain is a non specific, cysteine-endopeptidase isolated from papaya latex that is used in a wide variety of applications. When IgG molecules are incubated with papain in the presence of cysteine, one or more peptide bonds in the hinge region are split, producing three fragments of similar size: two Fab fragments and one Fc fragment. The Fc fragments can be separated from the Fab fragments using immobilised Protein A or by ion exchange chromatography.