Chemiluminescent RNA EMSA kit, LightShift™
Catalog # PIER20158
Supplier: Thermo Fisher Scientific
CAS Number:
Specifications
- Pk:1
- Description:LightShift™ Chemiluminescent RNA EMSA Kit, sufficient for 100 binding reactions and detection on 1000 cm2 of membrane (10 mini-gel blots)
- Environmentally Preferable:
Specifications
About this item
LightShift™ Chemiluminescent RNA EMSA Kit provides a non-radioactive solution for studying RNA-protein interactions using an electrophoretic mobility-shift assay (EMSA).
- Chemiluminescent detection comparable to radioactive detection is sensitive
- Develop X-ray films after 1 to 5 minute exposures thus being a time saving process
- Compatible with RNA probes biotinylated by different methods
- Eliminate waste concerns from radioactive RNA probes
Chemiluminescent RNA EMSA Kit is an in vitro technique for detection of protein-RNA interactions through changes in gel electrophoresis migration patterns. This kit uses biotinylated RNA probes and a chemiluminescent substrate system to achieve fast and safe detection of RNA-protein complexes with sensitivity equivalent to traditional 32P-isotopic methods.
Delivery: Kit includes 125 nM biotinylated IRE RNA control (35 µl), 10 µM unlabeled IRE RNA control (25 µl), 2 mg/ml cytosolic liver extract (50 µl), 10 mg/ml tRNA (100 µl), 10X REMSA binding buffer (1 ml), 50% glycerol (500 µl), 2M KCl (500 µl), 1M MgCl2 (500 µl), DTT lyophilized, nuclease-free water (1,5 ml), 5X REMSA loading buffer (1 ml), stabilized streptavidin-HRP (1,5 ml), luminol/enhancer solution (80 ml), stable peroxide solution (80 ml), nucleic acid detection blocking buffer (500 ml), 4X wash buffer (500 ml), and substrate equilibration buffer (500 ml).