The Pierce™ Rapid isotyping kit is a 5-minute lateral-flow assay with ELISA sensitivity for class and subclass determination of monoclonal antibodies.

• Stable for at least one year at room temperature
• Assay does not cross-react with foetal bovine serum
• Detect antibodies in tissue culture media or ascites fluid samples at concentration greater than 10 ng/ml

The assay is performed by simply adding a properly diluted tissue culture supernatant or mouse ascites sample to the well of the cassette. The gold conjugates embedded in the cassette form specific class- and subclass-soluble complexes with the antibodies in the sample. These complexes travel the length of the membrane and are resolved on the anti-isotype and class-specific antibody-impregnated membrane. Results are displayed as a red band indicating the antibody isotype or subclass.