Rabbit IgG polyclonal antibody for Cullin-3(CUL3) detection. Tested with WB in Human;Mouse;Rat.

Cullin-3(Cul 3), is a protein that in humans is encoded by the CUL3 gene. Kipreos et al.(1996) identified a conserved gene family, designated cullins, with at least 5 members in nematodes, 6 in humans, and 3 in S. cerevisiae. Human CUL3 is an ortholog of nematode cul3. Hartz(2012) mapped the CUL3 gene to chromosome 2q36.2 based on an alignment of the CUL3 sequence with the genomic sequence(GRCh37). Sumara et al.(2007) found that KLHL9, KLHL13, and CUL3 interacted directly in a 370-kD protein complex in HeLa cell lysates. Rondou et al.(2008) showed that interaction between KLHL12 and the CUL3 ubiquitin ligase complex directed ubiquitination of dopamine receptor D4(DRD4). KLHL12 interacted directly with CUL3 and with the polymorphic intracellular loop-3 of D4. By immunoprecipitation analysis of HeLa cells and HCT116 human colon carcinoma cells expressing epitope-tagged proteins, Kigoshi et al.(2011) showed that KLHL7 interacted with CUL3 and ROC1(RBX1).

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

WB: The detection limit for CUL3 is approximately 0.5ng/lane under reducing conditions.
Tested Species: In-house tested species with positive results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested.
Optimal dilutions should be determined by end users.

Type: Primary
Antigen: Cullin3
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Rabbit
Isotype: IgG
Reactivity: Human, Mouse, Rat