You searched for: Microscopy and staining reagents
Avantor offers a broad line of reagent droppers, diagnostic kits, and antigens and antisera, for aid in identifying a variety of microscopic organisms. The visualization of bacteria by means of staining procedures is an important tool for any microbiologist to have at their disposal, and these carefully standardized and reproducible stain reagents help to ensure accurate and easily interpretable results. Stains are also available to provide early detection of positive blood cultures by fluorescence procedures.
Embedding and mounting media
Supplier: Merck
The defined hardness and uniformity of the material to be cut are vital requirements in creating consistent quality in cutting. These requirements can be met using a range of suitable substances, such as paraffins, resins, gelatine and celloidin.
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Xylene substitute, Neo-Clear®, Sigma-Aldrich®
Supplier: Merck
Neo-Clear® is a non hazardous alternative to xylene. It is virtually odourless, compatible with most high purity solvents and is characterised by lower evaporation rates than xylene, making it a lower health risk.
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Decalcifier solutions, Osteomoll® and Osteosoft®, Sigma-Aldrich®
Supplier: Merck
Hard tissue like bones or teeth, and all specimens of calcified tissue like bone marrow punches require special pre-treatment before routine processing by a histological laboratory.
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Polyvinyl alcohol mounting medium with DABCO®, antifading, Supelco®
Supplier: Merck
Aqueous solution containing tris(hydroxymethyl)aminomethane, tris(hydroxymethyl)aminomethane hydrochloride, polyvinyl alcohol 22000, glycerol, and 1,4-diazabicyclo[2.2.2]octane.
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Tissue freezing media, PolyFreeze, Sigma-Aldrich®
Supplier: Merck
A support matrix or form of embedding medium for frozen sectioning. This medium freezes quickly supporting the tissue for sectioning at 3 μ and up with no cracking of the matrix at temperatures from –8 to –25 °C.
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Mounting medium, Entellan® new
Supplier: Merck
Entellan® new is a new mounting medium for cover slippers and is specially suited for commercial automated mounting instruments that operate with cover slippers. Its viscosity ranges between 250 and 600 mPas, 1,00869 has a small viscosity range. This narrow viscosity range improves and accelerates the application, as neither the dropping rate nor the volume need to be reset after the first run with a new bottle of Entellan® new.
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Water-free mounting medium, Neo-Mount®
Supplier: Merck
Water-free mounting media, such as Neo-Mount®, should be used for permanent mounting and long-term storage of samples. Slides processed in pathologyl laboratories need to be stored up to 10 years, whereby the staining results need to be stable and fading-out is not acceptable. Neo-Mount®, anhydrous mounting medium for microscopy is an extremely colour-stable mounting medium for microscopy, which is produced with solvents based on mixtures of aliphatic hydrocarbons. It contains an aromatic-free substitute for xylene, thus, Neo-Mount® needs to be combined with Neo-Clear® exclusively. Xylene must be avoided in the mounting step, as it will cause the slides to become cloudy and streaked. In addition, by placing the dehydrated slides on filter paper for approx. 1 minute prior to mounting, any excess Neo-Clear® should be circumvented, as air bubbles might arise under the cover slip. The same pre-condition should also be met when mounting specimens using cover slip machines; in this area, Neo-Clear® can be most efficiently eliminated by incubating the slides for 1 minute in an empty slide rack.
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Immersion oils
Supplier: ZEISS
Increases the resolving power of a microscope. Zeiss Immersol™ is a transparent oil with a refractive index of 1.518. It is free of fluorescence and halogen, and is certified according to ISO 8036-1/2.