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114 results for Western Blotting Reagents

You searched for: Western Blotting Reagents

Western Blotting Reagents

Nucleic acids or proteins in a complex sample can be visualized using electrophoresis and blotting procedures and the compatible reagents. Improving the overall efficiency of electrophoresis systems, these reagents accommodate a variety of blotting techniques and with antibodies provided against tens of thousands of different proteins. These methods are used in the fields of molecular biology, immunogenetics, and other molecular biology disciplines.

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Western blotting detection reagents, ECL western blotting substrate

Western blotting detection reagents, ECL western blotting substrate

Supplier: Thermo Fisher Scientific

ECL™ Western blotting substrate is an entry-level Western blotting substrate.

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Western blotting kit, ECL substrate, Pierce™

Western blotting kit, ECL substrate, Pierce™

Supplier: Thermo Fisher Scientific

The Fast Western Blot Kit, ECL Substrate contains optimised reagents that shorten the time which is required to perform a typical chemiluminescent Western blot from 4 hours down to approximately 55 minutes.

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Western blot stripping buffer, restore PLUS™

Western blot stripping buffer, restore PLUS™

Supplier: Thermo Fisher Scientific

Some antibodies are difficult to remove from Western blots and require Longer incubation times or incubation temperatures greater than 22 °C. Restore™ PLUS Western Blot Stripping Buffer was developed to reduce incubation times while keeping incubations at room temperature. High-affinity antibodies can be quickly and effectively stripped from Western blots without removing transferred proteins, thereby allowing multiple reprobes of the target.

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VWR® Fixing Solution for Isoelectric Focusing in Polyacrylamide Gel, Reag. Ph. Eur. 1138700

VWR® Fixing Solution for Isoelectric Focusing in Polyacrylamide Gel, Reag. Ph. Eur. 1138700

Supplier: VWR Chemicals

VWR®, Fixing solution for isoelectric focusing in polyacrylamide ge

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Western blots, pre-made, mouse subcellular fraction specific blot

Western blots, pre-made, mouse subcellular fraction specific blot

Supplier: G-Biosciences

The subcellular fractions from liver tissue are prepared by carefully dissecting out anatomically and functionally distinct regions of adult mouse livers.

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Nitrotyrosine BSA for immunoblotting experiments

Nitrotyrosine BSA for immunoblotting experiments

Supplier: Cayman Chemical

This Nitrotyrosine BSA can be used as a positive control for immunoblotting experiments using Nitrotyrosine Polyclonal Antibody and Nitrotyrosine Monoclonal Antibody.

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Peqlab peqGOLD AceGlow™, Blotting Reagent, Chemiluminescence Substrate

Peqlab peqGOLD AceGlow™, Blotting Reagent, Chemiluminescence Substrate

Supplier: VWR Collection

AceGlow™ Chemiluminescence Substrate is for the horseradish peroxidase-mediated detection of proteins and nucleic acids. There are two variants: AceGlow™ Essential for standard blots with a detection sensitivity of 1 picogram and a signal emission for up to 8 hours, and AceGlow™ with highest sensitivity down to 10 femtogram and amplified, long lasting light signal emission over 24 hours.

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Diluent for western blotting, Swift™ Western Diluent

Diluent for western blotting, Swift™ Western Diluent

Supplier: G-Biosciences

The Swift™ Western Diluent is a solution that reduces the blocking and antibody incubations on Western blot membranes to <60 minutes. Swift™ Western Diluent generates comparable results to traditional Western blotting procedures and other commercial “fast” Western blotting kits.

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Chemiluminescent acridinium esters

Chemiluminescent acridinium esters

Supplier: Enzo Life Sciences

Chemiluminescent acridinium esters are commonly used to label antibodies, proteins, nucleic acids, and some peptides, and are frequently used as detection reagents in immunoassays.

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Human cancer cell line blot

Human cancer cell line blot

Supplier: G-Biosciences

Ready to screen human cancer cell line blots.

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Eye tissue region blot, single species

Eye tissue region blot, single species

Supplier: G-Biosciences

These are ready to screen, normal tissue, eye tissue region blots from rat and rabbit. The eye tissue specific regions are prepared by carefully dissecting out anatomically and functionally distinct regions of adult rat and rabbit eyes.

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Normal cell line blots, human

Normal cell line blots, human

Supplier: G-Biosciences

Ready to screen, normal tissue human cell line blots from epithelial, endothelial, skin, and muscle tissue.

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CYTAG™ CGH labelling kits

CYTAG™ CGH labelling kits

Supplier: Enzo Life Sciences

The CYTAG™ CGH labeling kit produces high quality data for comparative genomic hybridisation (aCGH) using as little as 0,25 µg of genomic DNA, without a need for pre-amplification.

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IHC chromogen substrate (AEC, stable), HIGHDEF®

Supplier: Enzo Life Sciences

HIGHDEF® red IHC chromogen can be used in conjunction with peroxidase-based immunostaining systems.

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Immunoblotting kits, MaxTag™

Immunoblotting kits, MaxTag™

Supplier: Rockland Immunochemicals

These accurate and sensitive kits are ideal for Western blotting or dot blotting methods. They offer flexibility with two unique substrates: Bright Blue TMB substrate and Stable Brown DAB substrate. Components are colour-coded for error-free results. With MaxTag™ you can run up to 40 (10×10cm) immunoblots.

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Western blot stripping buffer, Western ReProbe™

Western blot stripping buffer, Western ReProbe™

Supplier: G-Biosciences

Western ReProbe™ is a single component system that is specifically formulated to dissociate and remove antibodies from membrane-bound proteins without destroying the antigenic binding affinity. Western ReProbe™ also enables the ability to reuse Western blots.

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Western ReProbe™ PLUS

Western ReProbe™ PLUS

Supplier: G-Biosciences

Western ReProbe™ PLUS allows for the removal of stubborn, high affinity antibodies from membrane bound proteins without destroying the antigenic binding affinity. The membrane bound protein is retained on the membrane and the matching antibodies are washed away. Once the antigen-antibody bonds are broken, the membrane bound protein is free to accept new antibodies.

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Single tissue blots, multiple species

Single tissue blots, multiple species

Supplier: G-Biosciences

Single tissue and multiple species blot contains four protein lanes with an identical GenLysate™ tissue lysate from human, mouse and rat species, and a protein marker lane.

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Cell extract HL60 (non-induced)

Supplier: Enzo Life Sciences

Whole-cell extract of human HL-60 leukemia cells.

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Immunodetection reagents, femtoLUCENT™ PLUS

Immunodetection reagents, femtoLUCENT™ PLUS

Supplier: G-Biosciences

The femtoLUCENT™ PLUS Reagent Kit facilitates the quick and sensitive non-isotopic detection of proteins on transfer membranes.

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picoLUCENT™ PLUS-HRP Kit

picoLUCENT™ PLUS-HRP Kit

Supplier: G-Biosciences

The picoLUCENT™ PLUS-HRP kit is based on an ultra sensitive luminol substrate that produces chemiluminescence upon reaction with horseradish peroxidase. The chemiluminescence light emission can be recorded by a short exposure to autoradiography films.

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Kidney tissue region blot, single species

Kidney tissue region blot, single species

Supplier: G-Biosciences

These are ready to screen, normal tissue, kidney tissue region blots from mouse and rat. The kidney tissue specific regions are prepared by carefully dissecting out anatomically and functionally distinct regions of adult mouse and rat kidney.

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picoLUCENT™ PLUS-AP kit

picoLUCENT™ PLUS-AP kit

Supplier: G-Biosciences

The picoLUCENT™ PLUS-HRP kit is based on an ultra sensitive luminol substrate that produces chemiluminescence upon reaction with alkaline phosphatase. The chemiluminescence light emission can be recorded by a short exposure to autoradiography films.

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VWR® VisiGlo™ HRP Chemiluminescent Substrate Kits

VWR® VisiGlo™ HRP Chemiluminescent Substrate Kits

Supplier: VWR Chemicals

VisiGlo™ and Visiglo Plus™ HRP chemiluminescent substrates are luminol-based chemiluminescent substrates designed for rapid and sensitive detection of peroxidase-labelled conjugates. Sensitivity ranges from picogram levels, captured by VisiGlo™ HRP chemiluminescent substrate, down to femtogram levels, exhibited by VisiGlo™ Prime and VisiGlo™ Select. VisiGlo™ Select displays the highest sensitivity, ideal for detecting low abundance proteins. The signal generated by substrate incubation is sustained between 8 to 10 hours allowing you to perform multiple exposures over an extended period of time.

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Mouse single tissue blot, single species

Mouse single tissue blot, single species

Supplier: G-Biosciences

This blot contains two protein lanes, a protein marker lane and a lane with one GenLysate™ sample. After the proteins are resolved, they are transferred to a PVDF membrane using Western Transfer Buffer. The blots are ready to be blocked and probed with the antibodies of choice.

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Rat single tissue blot, single species

Rat single tissue blot, single species

Supplier: G-Biosciences

This blot contains two protein lanes, a protein marker lane and a lane with one GenLysate™ sample. After the proteins are resolved, they are transferred to a PVDF membrane using Western Transfer Buffer. The blots are ready to be blocked and probed with the antibodies of choice.

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Multiple tissue blot, single species

Multiple tissue blot, single species

Supplier: G-Biosciences

These multiple tissue, single species blots permit researchers to visualise the tissue distribution of their protein in a particular species. These have been successfully used for the analysis of a wide variety of proteins. Each blot has multiple tissue types from either human, mouse or rat species.

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SuperSignal™, Pierce™ Fast Western Blotting Kit, West Femto

SuperSignal™, Pierce™ Fast Western Blotting Kit, West Femto

Supplier: Thermo Fisher Scientific

The Pierce™ Fast Western Blot Kit, SuperSignal™ West Femto Substrate contains optimised reagents that shorten the time to perform a typical Western blot from 4 hours to ~60 minutes.

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Transfer pads for western blotting, Swift™

Transfer pads for western blotting, Swift™

Supplier: G-Biosciences

SWIFT™ transfer pads are treated with a proprietary electrolyte buffer to enhance Western blot transfer efficiency.

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Western blots, pre-made, human single tissue single species

Western blots, pre-made, human single tissue single species

Supplier: G-Biosciences

Ready-to-screen, human, single tissue blots, from both normal and tumour tissues. G-Biosciences pre-made blots are prepared with the appropriate GenLysate™ and 50 μg protein is loaded onto a 4 to 20% denaturing polyacrylamide gel, along with a prestained molecular weight marker. After the proteins are resolved, they are transferred to a PVDF membrane, using our Western transfer buffer.

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