You searched for: Nucleic Acid Reagents
Nucleic acid reagents form the foundation of genetic analysis and molecular biology, playing a pivotal role from cloning to gene expression studies. Delve into our collection featuring high-fidelity enzymes for end-point PCR, advanced reagents for isothermal amplification, and comprehensive kits for nucleic acid purification. For precision in quantitative assays, explore our selection of qPCR and RT-qPCR kits, primed for unparalleled accuracy. Our portfolio also includes next-generation sequencing reagents, offering cutting-edge solutions for genomic sequencing and personalized medicine advancements.
VWR® Fast extract genotyping PCR kit
Supplier: VWR Chemicals
This fast extract genotyping PCR kit consists of a fast extraction DNA solution and VWR® Red Taq DNA polymerase 2X master mix. It is ideal for genotyping of DNA extracted from mammalian tissues, plant material, saliva or bacteria, providing PCR-ready DNA in 8 minutes and genotyping results in less than 1,5 hours.
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TriLink® N6-Methyladenosine-5'-Triphosphate
Supplier: TriLink BioTechnologies
N6-methyl adenosine (N6-methyl ATP) is a base modified analog of adenosine and is found as a minor nucleoside in natural RNAs (Meyer et al.). N6-methyl ATP can substitute for ATP in some biological systems, and is a potent agonist for P2Y-purinoceptors in the guinea pig, taenia coli (Burnstock et al.).
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TriLink® Uridine-5'-Triphosphate
Supplier: TriLink BioTechnologies
This nucleotide consists of an unmodified base, a ribose, and a 5′ triphosphate group for synthesis of RNA molecules.
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AMPIGENE® cDNA synthesis kit
Supplier: ENZO LIFE SCIENCES
AMPIGENE® cDNA Synthesis Kit for efficient and sensitive cDNA synthesis, for downstream qPCR.
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Restriction enzymes, Eco32I (EcoRV) (HC), Fermentas
Supplier: Thermo Fisher Scientific
ATC sites and cuts best at 37 °C in R buffer Isoschizomers: EcoRV.
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T4 DNA polymerase
Supplier: Thermo Fisher Scientific
T4 DNA Polymerase catalyses the 5'→3' synthesis of DNA from a primed single-stranded DNA template.
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Bio-11-CTP
Supplier: ENZO LIFE SCIENCES
Bio-11-CTP (Biotin-11-cytidine-5’-triphosphate) can replace CTP for in vitro transcription reaction catalysed by T3, T7 or SP6 RNA polymerases.
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Bio-16-UTP
Supplier: ENZO LIFE SCIENCES
Bio-16-UTP (Biotin-16-uridine-5’-triphosphate) can replace UTP for in vitro transcription reaction catalysed by T3, T7 or SP6 RNA polymerases.
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Restriction enzymes, SmaI, FastDigest™, Fermentas
Supplier: Thermo Fisher Scientific
Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.
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VWR®, Red Taq DNA polymerase
Supplier: VWR Chemicals
Red Taq DNA Polymerase is a blend of Taq DNA polymerase combined with an inert red dye. The dye enables quick visual recognition of reactions to which enzyme has been added, as well as confirmation of complete mixing.
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Restriction enzymes, BamHI, FastDigest™, fermentas
Supplier: Thermo Fisher Scientific
Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.
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TriLink® T7 RNA Polymerase
Supplier: TriLink BioTechnologies
T7 RNA Polymerase is a wild-type enzyme to synthesize RNA from DNA templates by in vitro transcription.
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BioProbe® 3’-Oligonucleotide labeling kit with bio-16-ddUTP
Supplier: ENZO LIFE SCIENCES
Terminal labeling is an ideal procedure for the labeling of oligonucleotides with haptens such as biotin or fluorescein. An oligonucleotide can be synthesized using standard, commercially available reagents and labeled after synthesis in a simple and reproducible enzyme reaction.
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TriLink® CleanCap® Reagent AU
Supplier: TriLink BioTechnologies
CleanCap® Reagent AU is designed for the co-transcriptional capping of self-replicating or self-amplifying (saRNA) to produce an optimal Cap 1.
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TriLink® Engineered RNase Inhibitor
Supplier: TriLink BioTechnologies
Engineered RNase Inhibitor is a protein to inhibit RNase activities to prevent RNA degradation in IVT.
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VWR®, GC Rich TEMPase Hot start DNA polymerase and MasterMix
Supplier: VWR Chemicals
VWR® TEMPase Hot Start DNA Polymerases are highly stable polymerases, featuring higher specificity, superior sensitivity and greater yields compared to standard DNA polymerases. These features make them well suited for the detection of low abundance targets. Other uses include screening, amplification of GC-rich sequences, multiplex PCR, direct PCR and qPCR.
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Restriction enzymes, AgeI, FastDigest™, Fermentas
Supplier: Thermo Fisher Scientific
Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.
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VWR®, PCR buffers
Supplier: VWR Chemicals
An optimal buffer system is critical for the performance of successful PCR. VWR® Taq DNA Polymerase kits generally include two different buffers, Key Buffer and Extra Buffer, which are suited for different PCR needs. All buffers contain Tris and 15 mM MgCl₂ (1,5 mM MgCl₂ final concentration). Additional MgCl₂ for easy optimisation is included in a separate vial.
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Restriction enzymes, NotI, 10 U/µl, Fermentas
Supplier: Thermo Fisher Scientific
GGCCGC sites and cuts best at 37 °C in O buffer.
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Restriction enzymes, NheI, FastDigest™, fermentas
Supplier: Thermo Fisher Scientific
Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.
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Restriction enzymes, SpeI, FastDigest™, fermentas
Supplier: Thermo Fisher Scientific
Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.
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Fast probe master mixes
Supplier: Biotium
A qPCR master mix containing Cheetah™ Taq hotstart DNA polymerase, suitable for probe-based real-time PCR applications.
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TriLink® Biotin-16-Aminoallyluridine-5'-Triphosphate
Supplier: TriLink BioTechnologies
Biotin-16-Aminoallyluridine-5'-Triphosphate (Biotin-16-UTP, Biotin-16-AA-UTP) can be incorporated via a number of RNA polymerases, including SP6-, T3- and T7-RNA polymerase to form labeled RNA.
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TriLink® 2'-Fluoro-2'-deoxycytidine-5'-Triphosphate
Supplier: TriLink BioTechnologies
2'-Fluoro NTPs are being utilised in an increasing number of applications in research and new drug development. 2'-Fluoro-dCTP is incorporated in both DNA and RNA constructs to improve in vivo stability.
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Bio-17-ATP
Supplier: ENZO LIFE SCIENCES
Bio-17-ATP (Biotin-17-adenosine-5’-triphosphate) can replace ATP for in vitro transcription reaction catalysed by T3, T7 or SP6 RNA polymerases.
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Restriction enzymes, NotI, FastDigest™, fermentas
Supplier: Thermo Fisher Scientific
Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.
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dNTP mix
Supplier: Biotium
Highly purified mix of dNTPs (>99% by HPLC) in 0,6 mM Tris-HCl (pH 7,5); each dNTP is at a concentration of 10 or 25 mm in the solution provided.
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BioProbe® 3’-Oligonucleotide labeling kit with fluorescein-12-ddUTP
Supplier: ENZO LIFE SCIENCES
Terminal labeling is an ideal procedure for the labeling of oligonucleotides with haptens such as biotin or fluorescein. An oligonucleotide can be synthesized using standard, commercially available reagents and labeled after synthesis in a simple and reproducible enzyme reaction.
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AMPIGENE® Taq mix
Supplier: ENZO LIFE SCIENCES
Optimised standard PCR mix for enhanced speed, yield, and specificity. Used for standard PCR applications.
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VWR®, dNTPs
Supplier: VWR Chemicals
Ready to use molecular biology grade dNTP mixes and dNTP sets. The dNTP mix is designed to save hands-on time for researchers, and reduce the possibility of contamination by reducing pipetting. dNTP solutions are also available in sets of four individual dNTPs, each 100 mm. Both are convenient for use in DNA polymerisation reactions, DNA labelling and sequencing processes.