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680 výsledky pro Nucleic Acid Reagents

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Nucleic Acid Reagents

Nucleic acid reagents form the foundation of genetic analysis and molecular biology, playing a pivotal role from cloning to gene expression studies. Delve into our collection featuring high-fidelity enzymes for end-point PCR, advanced reagents for isothermal amplification, and comprehensive kits for nucleic acid purification. For precision in quantitative assays, explore our selection of qPCR and RT-qPCR kits, primed for unparalleled accuracy. Our portfolio also includes next-generation sequencing reagents, offering cutting-edge solutions for genomic sequencing and personalized medicine advancements.

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TriLink® 2'-Deoxy-P-nucleoside-5'-Triphosphate

TriLink® 2'-Deoxy-P-nucleoside-5'-Triphosphate

Supplier: TriLink BioTechnologies

2'-Deoxy-P-nucleoside is a degenerate base which behaves as a universal pyrimidine and therefore base pairs with both adenine and guanine. This feature makes it a powerful tool in mutagenesis studies.

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Restriction enzymes, SfiI, Fermentas

Restriction enzymes, SfiI, Fermentas

Supplier: Thermo Fisher Scientific

NGGCC sites and cuts best at 50 °C in G buffer.

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TriLink® 5-Propargylamino-2'-deoxyuridine-5'-Triphosphate

TriLink® 5-Propargylamino-2'-deoxyuridine-5'-Triphosphate

Supplier: TriLink BioTechnologies

5-Propargylamino-2'-deoxyuridine can be enzymatically incorporated into DNA and cDNA to introduce primary amine groups. Primary amines can be labeled with NHS ester fluors and haptens for detection and immobilisation of the resulting DNA strand.

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TriLink® N7-Methyl-3'-O-Methyl-Guanosine-5'-Triphosphate-5'-Guanosine

TriLink® N7-Methyl-3'-O-Methyl-Guanosine-5'-Triphosphate-5'-Guanosine

Supplier: TriLink BioTechnologies

TriLink® N7-Methyl-3'-O-Methyl-Guanosine-5'-Triphosphate-5'-Guanosine

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Restriction enzymes, NdeI, Fermentas

Restriction enzymes, NdeI, Fermentas

Supplier: Thermo Fisher Scientific

TATG sites and cuts at 37 °C in O buffer (Isoschizomers: FauNDI).

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PCR decontamination kit

Supplier: ENZO LIFE SCIENCES

Rapid removal of contaminating DNA in PCR mastermixes, without reduction in PCR sensitivity.

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Restriction enzymes, EcoRI, FastDigest™, Fermentas

Restriction enzymes, EcoRI, FastDigest™, Fermentas

Supplier: Thermo Fisher Scientific

Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.

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TriLink® N1-Propylpseudouridine-5'-Triphosphate

TriLink® N1-Propylpseudouridine-5'-Triphosphate

Supplier: TriLink BioTechnologies

TriLink® N1-Propylpseudouridine-5'-Triphosphate

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Restriction enzymes, BamHI, HC, Fermentas

Restriction enzymes, BamHI, HC, Fermentas

Supplier: Thermo Fisher Scientific

GATCC sites and cuts best at 37 °C in its own unique buffer.

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AMPIGENE® 1-Step RT-PCR kit

Supplier: ENZO LIFE SCIENCES

AMPIGENE® 1-Step RT-PCR Kit for optimised and efficient cDNA synthesis and PCR in a single tube.

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Bio-11-CTP

Bio-11-CTP

Supplier: ENZO LIFE SCIENCES

Bio-11-CTP (Biotin-11-cytidine-5’-triphosphate) can replace CTP for in vitro transcription reaction catalysed by T3, T7 or SP6 RNA polymerases.

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Bio-16-UTP

Bio-16-UTP

Supplier: ENZO LIFE SCIENCES

Bio-16-UTP (Biotin-16-uridine-5’-triphosphate) can replace UTP for in vitro transcription reaction catalysed by T3, T7 or SP6 RNA polymerases.

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AMPIGENE® dNTP Mix

Supplier: ENZO LIFE SCIENCES

Ultra-pure, stable, and suitable for a variety of PCR applications.

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AMPIGENE® qPCR 1-Step Probe Kit Hi-ROX

Supplier: ENZO LIFE SCIENCES

Optimised and efficient cDNA synthesis and probe-based qPCR in a single tube.

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Blur 8 BioProbe®

Supplier: ENZO LIFE SCIENCES

The Blur 8 BioProbe® labeled probe is prepared by nick translation of a clone of the human alu repeat sequence. Fragment size range: 100-1000 base pairs (as estimated by agarose gel electrophoresis).

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Taq DNA polymerase, 1000 U

Supplier: Genscript

Taq DNA polymerase is a thermostable DNA polymerase isolated from an E. coli strain that carries the Taq DNA polymerase gene.

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Restriction enzymes, NcoI, FastDigest™, fermentas

Restriction enzymes, NcoI, FastDigest™, fermentas

Supplier: Thermo Fisher Scientific

Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.

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VWR® One-Step RT-qPCR Kit

VWR® One-Step RT-qPCR Kit

Supplier: VWR Chemicals

VWR® One-Step RT-qPCR Kit is designed to facilitate rapid, sensitive, and precise detection and quantification of various RNA sequences via dye-based qPCR. It features a distinctive universal passive reference dye that is compatible with ROX-dependent and ROX-independent qPCR instruments.

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TriLink® Sulf Cyanine5 Aminoallyluridine-5'-Triphosphate

TriLink® Sulf Cyanine5 Aminoallyluridine-5'-Triphosphate

Supplier: TriLink BioTechnologies

Cyanine 5-Aminoallyluridine-5'-Triphosphate is a substrate for T7 RNA polymerase, allowing the incorporation of cyanine 5 into RNA and mRNA during in vitro transcription. Cyanine 5 incorporation allows for fluorescent detection of RNA and mRNA independent of protein translation.

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Restriction enzymes, NotI, 10 U/µl, Fermentas

Restriction enzymes, NotI, 10 U/µl, Fermentas

Supplier: Thermo Fisher Scientific

GGCCGC sites and cuts best at 37 °C in O buffer.

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TriLink® 5-Methylcytidine-5'-Triphosphate

TriLink® 5-Methylcytidine-5'-Triphosphate

Supplier: TriLink BioTechnologies

This nucleotide consists of a modified base, a ribose, and a 5′ triphosphate group.

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TriLink® 2'-Deoxycytidine-5'-Triphosphate

TriLink® 2'-Deoxycytidine-5'-Triphosphate

Supplier: TriLink BioTechnologies

This nucleotide consists of an unmodified base, a deoxyribose, and a 5′ triphosphate group for synthesis of DNA molecules.

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TriLink® 2'-Deoxyguanosine-5'-Triphosphate

TriLink® 2'-Deoxyguanosine-5'-Triphosphate

Supplier: TriLink BioTechnologies

This nucleotide consists of an unmodified base, a deoxyribose, and a 5′ triphosphate group for synthesis of DNA molecules.

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Restriction enzymes, Eco32I (EcoRV) (HC), Fermentas

Restriction enzymes, Eco32I (EcoRV) (HC), Fermentas

Supplier: Thermo Fisher Scientific

ATC sites and cuts best at 37 °C in R buffer Isoschizomers: EcoRV.

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VWR®, Taq DNA polymerase, 500 U

VWR®, Taq DNA polymerase, 500 U

Supplier: VWR Chemicals

Taq DNA Polymerase is ideal for general PCR and primer extension reactions to amplify DNA templates with high sensitivity. PCR can be initiated from as few as 6 primed templates. Simply add 0,5 µl of Taq DNA Polymerase to a 25 µl PCR reaction.

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AccuStart™ master mixes, long range SuperMix

AccuStart™ master mixes, long range SuperMix

Supplier: Quantabio

Superior sensitivity and multiplexing for DNA amplification of long targets.

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Restriction enzymes, PfoI, Fermentas

Restriction enzymes, PfoI, Fermentas

Supplier: Thermo Fisher Scientific

CCNGGA sites and cuts best at 37 °C in Tango buffer.

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Digoxigenin-UTP, alkali-stable

Supplier: ENZO LIFE SCIENCES

Digoxigenin-labeled nucleotide suitable for non-radioactive labeling of RNA probes.

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Restriction enzymes, AgeI, FastDigest™, Fermentas

Restriction enzymes, AgeI, FastDigest™, Fermentas

Supplier: Thermo Fisher Scientific

Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.

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VWR®, TEMPase Hot Start 2× master mix

VWR®, TEMPase Hot Start 2× master mix

Supplier: VWR Chemicals

TEMPase Hot Start DNA Polymerase Master Mix and Blue TEMPase Master Mix are good alternatives to TEMPase Hot Start DNA Polymerase. These master mixes offer easy reaction assembly at room temperature, reduced set-up time and fewer handling steps, which lead to increased reproducibility. As a consequence TEMPase Hot Start DNA Polymerase Master Mix is highly suited to standard tests.

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