You searched for: Nucleic Acid Reagents

Supplier: MP Biomedicals

MPure™ Bacterial DNA Extraction Kit is used with the MPure-12™ Automated Nucleic Acid Purification System for extraction and purification of genomic DNA from both gram-positive and gram-negative bacteria. Nucleic acids extracted and purified from MPure™ Bacterial DNA Extraction Kit can be used in a number of downstream application including: PCR, qPCR, Sequencing (NGS), Microarray, RFLP and Southern Blot Analysis.

Supplier: Thermo Fisher Scientific

The Maxima™ SYBR® Green/Fluorescein qPCR Master Mix (2X) is a ready to use solution optimised for quantitative real time PCR and two-step real time RT-PCR on most real time PCR machines. The master mix includes Maxima™ hot start Taq DNA polymerase and dNTPs in an optimised PCR buffer. It contains SYBR® Green I dye and is supplemented with fluorescein passive reference dye. Fluorescein is included into the master mix to serve as an internal reference for normalisation
of the SYBR Green I fluorescent signal between different wells to compensate for any instrument or pipetting variation. dUTP is included in the mix for optional carryover contamination control using uracil-DNA glycosylase (UDG)*. Maxima™ hot start Taq DNA polymerase in combination with the optimised buffer ensures PCR specificity and sensitivity. The SYBR® Green I intercalating dye allows for DNA detection and analysis without using sequence specific probes. The use of Maxima™ SYBR® Green/Fluorescein qPCR Master Mix in real time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates.

Supplier: PanReac AppliChem

TRItidy G™ is based on the single step simultaneous isolation of RNA, DNA and proteins from cell and tissue samples (Chomczynski, P. (1993), BioTechniques 15, p.532 - 537). RNA is selectively retained in the aqueous phase during acidic GuSCN/phenol extraction; DNA and proteins are isolated from the organic phase by ethanol and isopropanol precipitation, respectively.

Supplier: MP Biomedicals

The FastDNA™ Kit quickly and efficiently isolates high quality genomic DNA from plants, animals, bacteria, yeast, algae, and fungi using a silica spin filter method. It can be used with the FastPrep®-24 or FastPrep® FP120 instrument to lyse and subsequently isolate DNA from up to 200 mg of almost any sample in less than 30 minutes.

Supplier: OMEGA BIO-TEK

Mag-Bind® RXNPure Plus Kit allows rapid and reliable isolation DNA from PCR and enzymatic reactions with high recovery rates. The system combines proprietary chemistries with the reversible nucleic acid-binding properties of magnetic beads that selectively bind PCR amplicons 100 bp and larger, and eliminate excess nucleotides, primers and small, non targeted amplification products, such as primer dimers.

Supplier: OMEGA BIO-TEK

The E.Z.N.A.® DNA/RNA isolation kit is designed for the simultaneous isolation of both genomic DNA and total RNA from the same cells or tissues. The sample is first lysed and homogenised in a special denaturing buffer, spun to pellet RNA and undigested particles, and then the supernatant is applied to a HiBind® DNA spin column to bind DNA. The RNA pellet is dissolved and purified by a HiBind RNA spin column. Since there is no need to divide the sample into two parts for separate purification procedures, maximum yield of DNA and RNA can be purified from the entire sample.

Supplier: Biotium

Digoxigenin-dUTP, alkali stable

Supplier: MP Biomedicals

SPINeasy DNA Kit for Water is a high-performance water genomic DNA (gDNA) extraction kit based on silica-membrane spin-column technology. This kit enables quick isolation of gDNA from water in less than 30 min. Water samples are processed using our uniquely formulated Lysis Buffer W1 and Lysing Matrix E to effectively lyse various types of cells. Column W1 provided in the kit has high binding capacity and selectivity for gDNA. The combination of components in the kit extracts gDNA of high yield and purity that is ready for downstream analyses such as PCR, restriction digestion and sequencing.

Supplier: G-Biosciences

Aqueous mixture of dATP, dCTP, dGTP and dTTP each at a final concentration of 10 or 25 mM.

Supplier: G-Biosciences

Set consists of 100 mM aqueous solutions of dATP, dCTP, dGTP and dTTP each supplied in a separate vial. Also available separately.

Supplier: MP Biomedicals

Taq-&GO™ is a ready to use optimised reaction mix for PCR.

Supplier: Biotium

A qPCR master mix containing Evagreen® qPCR dye and Cheetah™ Taq hotstart DNA polymerase, suitable for qPCR using a fast cycling protocol.

Supplier: Thermo Fisher Scientific

Applications: Complete and ready to use reagent for the isolation of genomic DNA from solid and liquid samples of animal and plant origin

Supplier: Biotium

EvaGreen® Plus Dye has an improved signal-to-noise compared to original EvaGreen® dye, for higher sensitivity in DNA amplification applications.

Supplier: MP Biomedicals

Taq-&LOAD™ PCR Master Mix is an optimised 5X reaction mix for PCR containing recombinant Taq DNA polymerase, dNTPs, MgCl₂, and incubation and loading buffers. Also included are a compound increasing the density of the sample and a red/purple dye.

Supplier: MP Biomedicals

The Taq CORE kits are designed for the amplification of specific regions of DNA using PCR. The PCR CORE Kits - Direct Loading provide Taq DNA Polymerase, PCR Direct Loading buffers and dNTPs necessary for amplification and subsequent loading of samples on an agarose gel.

Supplier: Merck Millipore (Novagen)

KOD HiFi DNA Polymerase is a unique proofreading enzyme, isolated from the extreme thermophile Thermococcus kodakaraensis KOD1, which possesses superior processivity and fidelity that enables faster and more accurate PCR amplification than can be achieved with conventional enzymes.

Supplier: MP Biomedicals

Taq DNA Polymerase is a specialised thermostable enzyme isolated from the thermophilic bacterium Thermus aquaticus. The recombinant form of this enzyme is expressed in E. coli. It is highly processive, recombinant polymerase which catalyses the addition of mononucleotide units to the 3’-OH end of a primer chain. It remains functional even after prolonged incubation at 95 °C. This enzyme possesses 5’-3’ exonuclease activiy.

Supplier: Quantabio

The 10 mM dNTP mix is a solution of high purity deoxyribonucleoside triphosphates that has been functionally qualified for real-time quantitative PCR (qPCR). It is suitable for use in conventional end-point PCR, real time qPCR, first-strand cDNA synthesis, as well as other applications that require dNTP as substrate. The use of qPCR certified dNTPs is a simple but important measure to ensure consistent assay performance.

Supplier: G-Biosciences

Plasmid Screening ToothPick™ allows the rapid screening of bacteria for transformed plasmids. Simply pick a colony, add to Plasmid Screening ToothPick™ reagents and then analyse using restriction enzymes.

Supplier: G-Biosciences

Taq DNA Polymerase is a highly thermostable recombinant DNA polymerase derived from the thermophile Thermus aquaticus. The molecular weight of the recombinant protein is 94 kD. The Taq Polymerase is able to amplify DNA up to 5 kb with an elongation velocity of 0,9 to 1,2 kb/min at 70 to 75 °C. The error rate of this Taq Polymerase is ~2,2×10⁻⁵ nucleotide⁻¹ cycle⁻¹. Taq DNA polymerase catalyses the 5’→3’ synthesis of DNA. The enzyme has no detectable 3’→5’ proofreading exonuclease activity, and possesses low 5’→3’ exonuclease activity, which results in a 3’-dA overhang on the PCR product

Supplier: G-Biosciences

Pfu DNA Polymerase, derived from the hyperthermophilic archae Pyrococcus furiosus, has superior thermostability and proofreading properties compared to other thermostable polymerases. It has a molecular weight of 90 kDa and can amplify DNA targets up to 2 kb. The elongation velocity is 0,2~0,4 kb/min (70~75 °C). Pfu DNA Polymerase possesses 3' to 5' exonuclease proofreading activity that enables the polymerase to correct nucleotide misincorporation errors. This means that Pfu DNA Polymerase-generated PCR fragments will have fewer errors than Taq-generated PCR inserts. Using Pfu DNA Polymerase results in blunt-ended PCR products, which are ideal for cloning into blunt-ended vectors. Pfu DNA Polymerase is superior for techniques that require high-fidelity DNA synthesis.

Supplier: G-Biosciences

GET™ AGAROSE DNA method involves release of DNA fragments from gel pieces followed by capture of nucleic acids on the GET™ spin columns, washing, and elution of the clean nucleic acid fragments in a suitable buffer.

Supplier: G-Biosciences

OmniPrep™ for Fungi Kit isolates high quality genomic DNA from fungal samples.If used according to the protocols this kit purifies DNA from 1 to 2 g fungal tissues.

Supplier: G-Biosciences

GET™ Plasmid DNA Miniprep Kit isolates high quality plasmid DNA from 1 to 5 ml E. coli cultures. The kit utilises an enhanced DNA binding column to produce high yields of plasmid.

Supplier: G-Biosciences

Arrest™ Extraction Buffer is an optimised combination of various chaotropic agents and RNase inhibitors, which inhibits RNase rapidly. It may be used in conjunction with any RNA extraction method, including extractions based on phenol, chloroform and other organic solvents and detergents.

Supplier: G-Biosciences

Yeast Geno-DNA Template™ isolates high quality genomic DNA from yeast. The kit is based on a two-step lysis of yeast cells, using LongLife™ Zymolyase® and LongLife™ Proteinase K, followed by the removal of proteins and other cellular impurities by precipitation and centrifugation.

Supplier: G-Biosciences

Tri-Xtract™ is designed for the isolation of total RNA that is free from protein and DNA contamination. The isolated RNA is suitable for Northern blots, dot blot hybridisation, in vitro translation, RNase protection assays and poly (A+) selection.

Supplier: Biotium

The DNA Gel Extraction Kit is a silica-gel, spin column based DNA extraction kit designed to purify 40 bp to 40 kb DNA fragments from agarose gels in TAE or TBE buffer.

Supplier: G-Biosciences

OmniPrep™ for Blood Kit isolates high quality genomic DNA from blood samples, including whole blood, buffy coats, packed cells and bone marrow.