You searched for: Electrophoresis Buffers

Supplier: Rockland Immunochemicals

Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)

Supplier: Rockland Immunochemicals

SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis, describes a technique widely used in biochemistry to separate proteins according to their electrophoretic mobility (a function of the length of a polypeptide chain and its charge) and no other physical feature. SDS is an anionic detergent applied to protein sample to linearise proteins and to impart a negative charge to linearised proteins.

Supplier: Abcam

Immunoassay Blocking (BSA Free) (ab171535) maintains the activity of antibodies and other biomolecules adsorbed or immobilized to solid substrates.

Supplier: G-Biosciences

Blocking buffer is an enhanced blocking agent available in multiple formats. Superior™ Blocking Buffer contains a proprietary antigenically non-determinant protein for blocking Western blot membranes, blocking and storage of ELISA plates, and blocking prior to immunohistochemistry staining.

Supplier: G-Biosciences

Protein-Free™ Blocking buffer is a formulation of non-protein agents that eliminate non-specific binding sites in ELISA, blotting, immunohistochemistry and other applications. The absence of protein from the Protein-Free™ Blocking buffer eliminates problems associated with traditional protein based blockers, such as cross-reactivity and interference from glycosylated proteins.

Supplier: Rockland Immunochemicals

Saline-sodium citrate (SSC) buffer is most commonly used as a buffer for the hybridisation process and washing in experiments where spcies of nucleic or ribonucleic acid are to be matched to complimentary sequences (such as Southern and Nouthern blotting, in situ hybridisation, or DNA microarrays). SSC is used to help control the stringency of hybridisation in these steps.

Supplier: PanReac AppliChem

Powder mixture to prepare PBS buffer (10X) according to Dulbecco.

Supplier: G-Biosciences

The TBS solution is primarily used as a wash and blocking buffer diluent for Western blot, ELISA and immunoassays.

Supplier: Biotium

The TrueBlack® WB blocking buffer kit is a ready-to-use buffer system that provides optimal specificity, sensitivity, and background signal suppression for fluorescence-based western blotting (WB).

Supplier: Cayman Chemical

250 mM Tris-base, pH 7,4, 1,37 M sodium chloride, containing 27 mM KCl, 0,4 mM phenol red.

Supplier: G-Biosciences

BLOT-QuickBlocker™ is a modified milk protein that is highly soluble and does not inhibit peroxidase detection. The modified milk protein has high blocking efficiency with a clear background.

Supplier: Rockland Immunochemicals

20X SSPE buffer (3,0 M sodium chloride, 0,2 M sodium hydrogen phosphate, 0,02 M EDTA, pH 7,4) - DEPC treated.

Supplier: Enzo Life Sciences

In Situ Hybridization Buffer provides a high quality hybridization medium for in situ hybridization analyses using either DNA or oligonucleotide probes labeled with biotin-, digoxigenin- or fluorescein-modified nucleotides.

Supplier: Enzo Life Sciences

Hybridisation wash reagent provides a high quality hybridisation wash solution for in situ hybridisation analyses using DNA probes labeled with biotin, digoxigenin or fluorescein.

Supplier: Enzo Life Sciences

In situ hybridisation buffer for HPV probes (ready-to use) provides a high quality hybridisation medium for In situ hybridisation analyses using either DNA or oligonucleotide probes labeled with biotin-, or fluorescein-modified nucleotides.

Supplier: Thermo Fisher Scientific

20X TAE buffer is a space-saving stock solution that is ideal for casting and preparing Tris-Acetate EDTA running buffer used for agarose gel electrophoresis of nucleic acids.

Supplier: EDVOTEK

This 50X concentrated solution (Tris-acetate, EDTA, pH 7,8) is sufficient for making 5 L of diluted working buffer for making agarose gels and electrophoresis chamber buffer.

Supplier: OMEGA BIO-TEK

Running Buffer, TAE buffer solution 50X concentrate (TRIS-acetate-EDTA buffer)

Supplier: Thermo Fisher Scientific

These wash buffers are designed for use in dialysis, cross-linking, enzyme assays, ELISAs, immunohistochemistry, protein plate-coating, biotinylation applications.

Supplier: Thermo Fisher Scientific

BupH packs are pre-blended and pre-measured dry mixtures of buffers that are easy to prepare and used in protein purification and common laboratory techniques.

Supplier: G-Biosciences

BLOK™ Casein is a purified protein, used for blocking free binding sites in Western blot, ELISA plate well, immunohistochemistry and other immunochemical applications.

Supplier: Thermo Fisher Scientific

Pierce™ Lane Marker Non-Reducing Sample Buffer is a ready-to-use 5X SDS-PAGE sample loading buffer in a non-reducing formulation, with a pink dye buffer-front marker.

Supplier: Thermo Fisher Scientific

Blocker™ BLOTTO is a ready-to-use 5% solution of non fat powdered milk in Tris-buffered saline for Western blot, ELISA, IHC and other detection methods.

Supplier: PanReac AppliChem

Powder for 10X concentrated aqueous solution (170,28 g/L). Composed of 0,89 M Tris, 0,89 M boric acid and 0,02 M EDTA.Na₂.

Supplier: Thermo Fisher Scientific

North2South™ hybridisation buffer is an aqueous buffer which is used with the North2South™ chemiluminescent detection kit.

Supplier: PanReac AppliChem

Running Buffer, TBE buffer solution 5X concentrate (TRIS-borate-EDTA buffer)

Supplier: G-Biosciences

Running Buffer, Tris-Glycine [10X]

Supplier: Thermo Fisher Scientific

Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)

Supplier: G-Biosciences

BLOK™ BSA is a 10% BSA protein blocking solution.

Supplier: Thermo Fisher Scientific

These StartingBlock™ blocking buffers are PBS and TBS formulations of a proprietary protein that provides exceptional performance and broad compatibility with membrane (Western blot) and 96-well plate (ELISA) assays.