You searched for: Electrophoresis Buffers
The electrophoresis buffers maintain consistent pH levels during DNA, RNA, and other protein separation. Providing ions that transfer the applied current, the low charge solutions facilitate different molecular sizes to travel at various rates for analyses. Offering additional protection to proteins, the preparative substances prevent degradation for guaranteed sample viability and accurate testing. Available in various molecular weights, the best electrophoresis buffer can be selected based on the specific macromolecules being handled.
Buffer, TBS solution 10X concentrate (TRIS buffered saline) with phenol red, pH 7,4
Supplier: Cayman Chemical
250 mM Tris-base, pH 7,4, 1,37 M sodium chloride, containing 27 mM KCl, 0,4 mM phenol red.
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SSPE Buffer, 20X concentrate, pH 7,4
Supplier: Rockland Immunochemicals
20X SSPE buffer (3,0 M sodium chloride, 0,2 M sodium hydrogen phosphate, 0,02 M EDTA, pH 7,4) - DEPC treated.
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Hybridisation buffers
Supplier: G-Biosciences
The hybridisation buffers like Church & Gilbert’s, Denhardt solution, hybridisation denaturing solution, hybridisation neutralising solution, SSC, SSPE, TMAC Hyb solution and TNT Buffer are used in wide range of applications. Church & Gilbert’s Hybridization Buffer is prepared from molecular biology grade SDS (sodium dodecyl sulfate), monosodium phosphate (NaHPO₄), EDTA (ethylenediaminetetracetic acid) and bovine serum albumin (BSA) Fraction V using DNase, RNase and Protease-free water. It consists of 1mM EDTA, 1% BSA, 0,5M sodium phosphate monobasic, and 7% SDS (at a pH of 7,2).
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Blocking buffer, fish gelatin
Supplier: Biotium
Fish gelatin is commonly used in blocking solutions to minimise background noise in immunodetection procedures such as Western Blotting and immunostaining. Fish gelatin can be used to block non-specific binding sites on positively charged nylon or PVDF membranes or for blocking and dilution of antibodies during immunostaining procedures. Unlike BSA or milk, fish gelatin does not contain IgG or serum proteins that could cross-react with mammalian antibodies.
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Electrophoresis running buffers, Pierce™
Supplier: Thermo Fisher Scientific
These are space-saving stock solution that is ideal for quickly preparing running buffer used for gel electrophoresis.
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TAE buffer, 50X
Supplier: EDVOTEK
This 50X concentrated solution (Tris-acetate, EDTA, pH 7,8) is sufficient for making 5 L of diluted working buffer for making agarose gels and electrophoresis chamber buffer.
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TAE buffer (20X), Pierce™
Supplier: Thermo Fisher Scientific
20X TAE buffer is a space-saving stock solution that is ideal for casting and preparing Tris-Acetate EDTA running buffer used for agarose gel electrophoresis of nucleic acids.
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Tris-Glycine buffer
Supplier: EDVOTEK
For vertical polyacrylamide gel electrophoresis.
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North2South™ Hybridisation Buffer
Supplier: Thermo Fisher Scientific
North2South™ hybridisation buffer is an aqueous buffer which is used with the North2South™ chemiluminescent detection kit.
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Buffer, TAE solution 50X concentrate (TRIS-acetate-EDTA buffer)
Supplier: OMEGA BIO-TEK
Running Buffer, TAE buffer solution 50X concentrate (TRIS-acetate-EDTA buffer)
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Tris-Glycine native gel running buffer
Supplier: G-Biosciences
Running Buffer, Tris-Glycine [10X]
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Wash buffer, Pierce™
Supplier: Thermo Fisher Scientific
These wash buffers are designed for use in dialysis, cross-linking, enzyme assays, ELISAs, immunohistochemistry, protein plate-coating, biotinylation applications.
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VHB DNA Washing Buffer
Supplier: OMEGA BIO-TEK
VHB buffer must be diluted with ethanol before use.
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Pierce™ BupH™ Wash Buffers, Dry Blend Pack
Supplier: Thermo Fisher Scientific
BupH packs are pre-blended and pre-measured dry mixtures of buffers that are easy to prepare and used in protein purification and common laboratory techniques.
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Gel loading solution, 10X
Supplier: EDVOTEK
1 ml concentrate for 200 samples.
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Blocking solution, BLOK™ Casein
Supplier: G-Biosciences
BLOK™ Casein is a purified protein, used for blocking free binding sites in Western blot, ELISA plate well, immunohistochemistry and other immunochemical applications.
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Wash buffer, Pierce™ Fast Wash Buffer
Supplier: Thermo Fisher Scientific
Pierce™ Fast Wash buffer, 10X, for use with Pierce™ Fast Blocking buffer.
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Pierce™ Lane Marker Non-reducing Sample Buffer (5X)
Supplier: Thermo Fisher Scientific
Pierce™ Lane Marker Non-Reducing Sample Buffer is a ready-to-use 5X SDS-PAGE sample loading buffer in a non-reducing formulation, with a pink dye buffer-front marker.
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Blocking solution, BLOK™ BSA
Supplier: G-Biosciences
BLOK™ BSA is a 10% BSA protein blocking solution.
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Buffer, TBE solution 5X concentrate (TRIS-borate-EDTA buffer)
Supplier: PanReac AppliChem
Running Buffer, TBE buffer solution 5X concentrate (TRIS-borate-EDTA buffer)
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Buffer, TRIS-glycine-SDS solution 10X concentrate (TGS buffer) pH 8,3
Supplier: PanReac AppliChem
Running Buffer, TRIS-glycine-SDS buffer solution 10X concentrate (TGS buffer) pH 8,3
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Protein blocking solution, BLOK™ BLOTTO
Supplier: G-Biosciences
BLOK™ BLOTTO is a 5% novel modified milk protein solution used for Westerns, ELISA and dot blot blocking.
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Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer)
Supplier: Thermo Fisher Scientific
Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)
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Buffer, TBE (Tris-borate-EDTA) powder for 10X concentrated solution
Supplier: PanReac AppliChem
Powder for 10X concentrated aqueous solution (170,28 g/L). Composed of 0,89 M Tris, 0,89 M boric acid and 0,02 M EDTA.Na₂.
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Blocker™ Blocking Buffer, BLOTTO in TBS
Supplier: Thermo Fisher Scientific
Blocker™ BLOTTO is a ready-to-use 5% solution of non fat powdered milk in Tris-buffered saline for Western blot, ELISA, IHC and other detection methods.
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Native sample loading buffer, 2X
Supplier: G-Biosciences
Loading Buffer, Native sample loading buffer (2X)
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Pierce™ Fish Serum Blocking Buffer,Thermo Scientific
Supplier: Thermo Fisher Scientific
This Fish Serum blocking buffer is steelhead salmon serum in PBS that is especially useful as a blocking agent in IHC and other detection methods involving mammalian samples.
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Western Blot Transfer buffer, 10X, methanol-free, Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ Methanol-Free 10X Western Blot Transfer Buffer is a space-saving stock solution for preparing the methanol-free transfer buffer used with cooled, tank (wet) western blotting transfer apparatus.
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Tris-Borate-EDTA (TBE) buffer, 10X, Molecular biology grade
Supplier: PanReac AppliChem
10X concentrated solution of 0,89 M Tris, 0,89 M boric acid and 0,02 M EDTA.Na₂, pH 8,3 ±0,2.
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Pierce™ Blocking Buffers, Protein Free
Supplier: Thermo Fisher Scientific
These protein-free blocking buffers are either a PBS or a TBS formulation of a non protein compound that provides effective blocking for membrane based and plate based protein detection methods, resulting in extremely low background.