You searched for: Protein Purification

Supplier: Thermo Fisher Scientific

Bond-Breaker™ TCEP Solution (neutral pH) is a stable, 0,5 M solution of the thiol-free, phosphine-based TCEP compound, useful as a 10X stock for addition to SDS-PAGE sample loading buffers to reduce protein disulphide bonds.

Supplier: Thermo Fisher Scientific

Jacalin is an alpha-D-galactose binding lectin extracted from jack-fruit seeds (Artocarpus integrifolia). This lectin is a glycoprotein (approx. 40 kDa) composed of four identical subunits. Jacalin binds to human IgA1 (also called serum or secretory IgA) with high specificity (i.e., it does not bind IgG or IgM). Thus, Jacalin that has been immobilised on crosslinked beaded agarose is useful for purifying human IgA from serum or colostrum samples.

Supplier: Thermo Fisher Scientific

Pierce™ MS(PEG)_n reagents are methyl-terminated, polyethylene glycol compounds (n equals 4 to 24 PEG units) and TMS(PEG)₁₂ is a three-branched, polyethylene glycol compound (3× 12 PEG units). Both are activated as NHS esters for covalent pegylation of primary amines on proteins (eg. lysines) or assay surfaces.

Supplier: Thermo Fisher Scientific

Pierce™ GST Spin Purification Kit provides a fast, easy to use format for purifying GST-fusion proteins from cellular lysates.

Supplier: G-Biosciences

The amine reactive HOOK™ Agarose Coupling Kit is designed for the simple and efficient coupling of proteins and peptides to a solid support. The kit allows the generation of both a stable and reusable column for the affinity purification of antibodies, proteins and other protein interacting biomolecules.

Supplier: Thermo Fisher Scientific

Pierce™ Control Agarose Resin is underivatised beaded agarose support for pre-clearing protein immunoprecipitation samples.

Supplier: G-Biosciences

Recombinant streptavidin from Streptomyces avidinii, produced in E.coli.

Supplier: Thermo Fisher Scientific

Provides a complete set of reagents and a simple, fast and reproducible protocol to perform chromatin immunoprecipitation (ChIP) assays.

Supplier: G-Biosciences

This Sulphhydryl Immobilisation Kit is designed for the coupling of sulphhydryl containing peptides to a solid agarose support. The sulphhydryl coupling resin columns utilises iodoacetyl groups that specifically react with free sulphhydryls to form covalent, permanent thioether bonds.

Supplier: Thermo Fisher Scientific

An optimized buffer for aldehyde-hydrazide and aldehyde-alkoxyamine coupling reactions.

Supplier: G-Biosciences

Immobilised β-cyclodextrin is designed to allow refolding of recombinant proteins that were aggregated or formed inclusion bodies when expressed in the genetically engineered cells. The refolding of misfolded proteins is adapted from GroE chaperone refolding mechanism.

Supplier: BRUKER DALTRONIK

Pre-portioned HCCA matrix provides easy and convenient preparation of HCCA matrix solutions.

Supplier: Thermo Fisher Scientific

Methyl-PEG₄-thiol is a methyl- and sulfhydryl-terminated compound that contains a 4-unit polyethylene glycol (PEG) spacer and is used to modify surfaces such as quantum dots, monolayers and magnetic particles.

Supplier: G-Biosciences

Chelex beads are made of a styrene divinylbenzene copolymer containing paired iminodiacetate ions, which act as chelating groups in binding polyvalent metal ions.

Supplier: Thermo Fisher Scientific

MALDI Matrices are highly purified, recrystallised reagents packaged in convenient single-use tubes sufficient for analysing up to 96 samples.

Supplier: G-Biosciences

Immobilised Catalase is prepared by covalent coupling of catalase enzyme to 6% cross-linked agarose beads. Immobilised Catalase is used to remove hydrogen peroxide from biological samples for use in downstream applications which require absenceof hydrogen peroxide as it causes interference.

Supplier: Thermo Fisher Scientific

Pierce™ Co-Immunoprecipitation (Co-IP) Kit provides covalent antibody immobilisation and all the components necessary to perform a properly controlled co-IP experiment without antibody interference in the final product.

Supplier: Thermo Fisher Scientific

The Pierce™ Direct IP Kit uses an activated resin to covalently immobilise IP antibodies (any species or class) on agarose beads without the aid of Protein A/G, enabling immunoprecipitation without antibody interference.

Supplier: Thermo Fisher Scientific

Pierce F(ab')2 preparation kit uses immobilised pepsin protease to selectively digest whole human and other IgG antibodies to make F(ab')2 fragments that retain antigen binding activity.

Supplier: Thermo Fisher Scientific

The methyl-PEG_n-amine (MA[PEG]_n) PEGylation reagents are methyl ether-terminated PEG amines that are used for modifying proteins or surfaces such as beads, nanoparticles and self-assembled monolayers. These PEGylation reagents are homogenous compounds of defined molecular weight and spacer length, providing precision in optimising modification applications.

Supplier: Thermo Fisher Scientific

Carboxy-PEG₁₂-thiol, is a carboxyl- and sulphhydryl-terminated compound that contains a 12-unit polyethylene glycol (PEG) spacer and is used to modify surfaces such as quantum dots, monolayers and magnetic particles.

Supplier: Cytiva

Capto™ Phenyl (high sub) is used in the capture and intermediate stages of protein purification.

Supplier: Cytiva

Capto™ Q ImpRes is a strong anion exchange bioprocess medium, designed to meet the demands of modern large-scale manufacturers for fast, efficient, and cost-effective intermediate and polishing protein purification.

Supplier: Cytiva

Glutathione Sepharose™ 4B is designed for high capacity single-step purification of Glutathione S-Transferase (GST) tagged fusion proteins.

Supplier: Cytiva

IMAC Sepharose High Performance media is easily charged by the user with the metalion of choice for Immobilized Metal ion Affinity Chromatography (IMAC) and subsequent purification of polyhistidine tagged proteins.

Supplier: Cytiva

Capto™ DEAE is a strong anion exchange bioprocessing medium, designed to meet the demands of modern large-scale manufacturers for fast, efficient, and cost-effective capture and intermediate protein purification when high volume throughput is essential.

Supplier: Cytiva

Ni Sepharose™ High Performance affinity media for high resolution, high capacity purification of histidine-tagged proteins by immobilised metal ion affinity chromatography (IMAC).

Supplier: Cytiva

Capto™ Blue is an affinity medium for the capture of albumin, purification of enzymes, and recombinant proteins at laboratory and process scales.

Supplier: Cytiva

Glutathione Sepharose High Performance is used for the purification of GST-tagged proteins from bacterial lysates and other S-transferases or glutathione-dependent proteins.

Supplier: Cytiva

A chromatography resin for purification of recombinant proteins tagged with Strep-tag II and Twin-Strep-tag. These proteins bind very specifically to the immobilised Strep-Tactin XT ligand giving highly pure target protein.