You searched for: Protein Purification

Supplier: Cytiva

Sephadex™ G-75 is well established gel filtration medium for desalting and buffer exchange of large biomolecules >80 000 molecular weight.

Supplier: Cytiva

Good resolution separations of proteins and other biomolecules according to size, Wide separation range – separations in two molecular weight ranges from 1,000 up to 5 million, Excellent reproducibility and durability

Supplier: Cytiva

Epoxy-activated Sepharose™ 6B is a pre-activated medium for immobilisation of various ligands including sugars through coupling of hydroxy, amino or thiol groups on the ligand to Sepharose™ 6B via a 12-atom hydrophilic spacer arm. Medium is formed by reacting Sepharose™ 6B with 1,4-bis (2,3- epoxy-propoxy-) butane. It can be used to couple sugars and other carbohydrates via stable ether linkages to hydroxyl groups.

Supplier: Cytiva

SP Sephadex™ C-25 is a strong cation exchanger. The ion exchange group is a sulphopropyl group which remains charged and maintains consistently high capacity over the entire working range of pH 4 to 13.

Supplier: Cytiva

The 2-D Clean-Up Kit is designed to prepare samples for 2-D electrophoresis that otherwise produce poor 2-D spot-maps due to high conductivity, high levels of interfering substances, or low protein concentration. The reagents quantitatively precipitate proteins while leaving interfering substances, such as detergents, salts, lipids, phenolics, and nucleic acids, in solution.

Supplier: Cytiva

DEAE Sepharose™ Fast Flow is part of the Sepharose™ Fast Flow ion exchange platform. It is composed of cross-linked 6% agarose beads, with diethylaminoethyl (DEAE) weak anion exchange groups. Scale-up with DEAE Sepharose™ Fast Flow is straight forward.

Supplier: Cytiva

Capto™ L is a bioprocessing chromatography resin for the capture of antibodies and antibody fragments. It combines a rigid, high-flow agarose matrix with the immunoglobulin-binding recombinant protein, L ligand, which has a strong affinity to the variable region of antibody kappa light chains. It is suitable for the capture of a wide range of antibody fragments such as Fabs, single-chain variable fragments (scFv), and domain antibodies (Dabs).

Supplier: MP Biomedicals

Protein A is a recombinant Fc-binding protein from Staphylococcus aureus, Cowan strain-1 gene. The product is a carboxy truncated material containing 301 amino acid residues. The non-binding regions have been removed to minimise potenial steric interference.

Supplier: G-Biosciences

The concentrator is a high molecular weight polymer which will not migrate across the dialysis membrane. The concentrator is placed against the dialysis membrane of the Tube-O-DIALYZER™, where it rapidly absorbs water from the sample and reduces the sample volume. The concentrator can be used with dialysis bags and other dialysis devices.

Supplier: Cytiva

Biacore maintenance kits are convenient kits for proper instrument maintenance.

Supplier: Merck

Sephadex® G-50 is a gel filtration medium used in affinity chromatography, protein chromatrography and gel filtration chromatography.

Supplier: Merck

Chelex® 100 Chelating Resin is a weak cation exchange resin that has high selectivity, approximately 5,000:1, for copper, iron, and other heavy metal ions over monovalent cations. Chelex 100 Resin is made of a styrene divinylbenzene copolymer containing paired iminodiacetate ions, which act as chelating groups in binding polyvalent metal ions.

Supplier: Merck

Ion Exchanger I is a strong acid cation exchangers widely used in separation of noble earths and amino acids, water treatment, analysis and food industry. The bond strength of equivalent ions increases with decreasing diameter of the hydrated ion.

Supplier: Merck

Gelatin-agarose is an agarose in saline suspension used for affinity chromatography, protein chromatography and specialty resins.

Supplier: Merck

Sepharose Fast Flow is composed of macroporous, beaded, cross-linked agarose and modified with sulphopropyl (SP) strong cation exchange groups which remains charged and maintains consistently high capacity over the entire working range of pH 4 to 13.

Supplier: Merck

50% aqueous suspension in 1 M NaCl.

Supplier: Thermo Fisher Scientific

SMART Digest™ Chymotrypsin Kits are designed to provide a complimentary digestion option to the SMART Digest™ Trypsin Kits which incorporate trypsin functionality. The Chymotrypsin enzyme functionality allows digestion of proteins at different points of the amino acid chain compared to trypsin. This allows for different/additional structural information to be obtained when trying to characterise a protein. This option can also be used if a protein does not contain the specific point where trypsin cuts the protein chain.

Supplier: Thermo Fisher Scientific

Thermo Scientific™ SMART Digest™ Trypsin Kits are designed to provide a simple to use, fast, highly reproducible and robust process for digestion of proteins in a high throughput format, meeting the needs of biopharmaceutical, bioanalytical and QA/QC environments.

Supplier: Thermo Fisher Scientific

Thermo Scientific™ SMART Digest™ ImmunoAffinity (IA) Kits are designed to provide fast, simple, highly reproducible and sensitive immunocapture and protein digestion in a single well. These kits remove the challenges often associated with the process of biomarker quantitation, by providing a simple and easy capture-digestion workflow that takes only hours instead of overnight.

Supplier: Thermo Fisher Scientific

Thermo Scientific™ SMART Digest™ Proteinase K Kits are designed to provide a simple to use, fast, highly reproducible and robust process for digestion of proteins in a high throughput format, meeting the needs of biopharmaceutical, bioanalytical and QA/QC environments. This version of the kit utilises Proteinase K, which cleaves the peptide bond adjacent to the carboxyl gropu of aliphatic and aromatic amino acids with blocked alpha amino groups.

Supplier: LIGATRAP TECHNOLOGIES

LigaTrap® Human IgA Purification Kit with LT technology utilises novel, patented ligands, designed for IgA antibody purification. Kit provides all the necessary reagents for fast, convenient micro-scale purification of human IgA in just ten easy steps.

Supplier: LIGATRAP TECHNOLOGIES

LigaTrap® Mouse IgM Purification Kit with LT technology utilises novel, patented ligands, designed for IgM antibody purification. Kit provides all the necessary reagents for fast, convenient micro-scale purification of mouse IgM in just ten easy steps.