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You searched for: Protein Purification

Protein Purification

Protein purification systems are intended to isolate one or multiple proteins from a complex mixture. Working with cells, tissues, or whole organisms, the machines purify with predefined start points and editable method templates. With a single click, the compact designed equipment offers real-time run control and visualization. Suitable for even cold-room operations, the products enable you to perform purification of affinity-tagged proteins, antibodies, untagged or native proteins, as well as sample clean-up.

Immunoprecipitation, magnetic ChIP kit, Pierce™

Supplier: Thermo Fisher Scientific

The Pierce™ Magnetic ChIP Kit provides a convenient method for efficient isolation of chromatin-bound DNA by immunoprecipitation for subsequent quantitation by PCR.

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Recombinant protein A agarose, Pierce™

Recombinant protein A agarose, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Recombinant Protein A agarose is a beaded agarose resin to which a genetically engineered form of Protein A has been immobilised for use in IgG purification applications where the native ligand has undesirable effects.

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Monomeric avidin agarose, Pierce™

Monomeric avidin agarose, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Monomeric avidin agarose is immobilised onto 4% beaded agarose for purifying biotinylated proteins, peptides and other molecules.

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Affinity chromatography media, HisPur™ Ni-NTA Agarose

Affinity chromatography media, HisPur™ Ni-NTA Agarose

Supplier: Thermo Fisher Scientific

HisPur™ Ni-NTA resin is a high-capacity, high-performance nickel-IMAC resin for routine affinity purification of His-tagged fusion proteins.

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HisPur™ cobalt superflow agarose, Pierce™

HisPur™ cobalt superflow agarose, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ HisPur™ Cobalt Superflow Agarose is a tetradentate-chelating Superflow 6 support charged with divalent cobalt, designed for high-purity, large-scale purification of polyhistidine-tagged proteins.

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Monomeric avidin resin, UltraLink™, Pierce™

Monomeric avidin resin, UltraLink™, Pierce™

Supplier: Thermo Fisher Scientific

UltraLink™ Monomeric Avidin resin is ideal for affinity purification of biotinylated proteins, peptides and other molecules. Immobilisation of avidin monomers results in a purification resin with a much lower biotin-binding affinity than native tetrameric avidin, enabling recovery of biotinylated molecules using mild elution conditions.

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Affinity purification kits, Melon™ gel Monoclonal IgG purification kit

Affinity purification kits, Melon™ gel Monoclonal IgG purification kit

Supplier: Thermo Fisher Scientific

Melon™ Gel Monoclonal IgG Purification Kit is a high-yield, mild purification system for monoclonal antibodies from ascites or hybridoma cell culture supernatant, or for large scale antibody purification from serum.

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Affinity coupling resins, AminoLink™

Affinity coupling resins, AminoLink™

Supplier: Thermo Fisher Scientific

AminoLink™ coupling resin is crosslinked, 4% beaded agarose that has been activated with aldehyde groups to enable covalent immobilisation of antibodies and other proteins through primary amines.

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Immobilised Protein G, UltraLink™, Pierce™

Immobilised Protein G, UltraLink™, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Protein G UltraLink™ and Protein G Plus UltraLink™ resins are useful for purification of antibodies and immunoprecipitation (IP). Samples containing IgG are incubated with the resin-immobilised Protein G in a buffer that facilitates binding. After non-IgG and non-antigen components of the sample are washed from the resin, the bound IgG and antigen may be recovered by elution.

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Pierce™ Avidin Agarose

Pierce™ Avidin Agarose

Supplier: Thermo Fisher Scientific

Pierce™ Avidin agarose is purified avidin immobilised on crosslinked 6% beaded agarose for the affinity purification of biotinylated macromolecules.

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Immobilised protein A, UltraLink™, Pierce™

Immobilised protein A, UltraLink™, Pierce™

Supplier: Thermo Fisher Scientific

Protein A UltraLink™ and Protein A Plus UltraLink™ resins are extremely durable beaded polyacrylamide supports to which IgG-binding Protein A has been immobilised for use in IgG purification methods. Protein A resin is used to bind and purify IgG in gravity-flow, spin-column or medium pressure chromatography methods.

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Protein refolding kit, Pierce™

Protein refolding kit, Pierce™

Supplier: Thermo Fisher Scientific

The Pierce™ Protein Refolding Kit includes high-purity reagents and detailed instructions for using a matrix strategy to determine optimal buffer conditions for refolding recombinant proteins that have been denatured and solubilised from inclusion bodies.

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Micro immobilisation kit, GlycoLink™

Supplier: Thermo Fisher Scientific

Hydrazide-activated resin to immobilise glycoproteins for large-scale affinity purification.

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ReadiPrep™ Nickel NTA Magnetic Beads

Supplier: AAT BIOQUEST

ReadiPrep™ Nickel NTA magnetic beads provide a fast and simple solution for small-scale affinity purification of histidine-tagged proteins.

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Streptavidin agarose, high capacity, Pierce™

Streptavidin agarose, high capacity, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ High capacity streptavidin agarose consists of purified recombinant streptavidin that has been covalently immobilised at a high density onto high-quality crosslinked 6% beaded agarose.

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AminoLink™ Plus micro immobilisation kit, Pierce™

AminoLink™ Plus micro immobilisation kit, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ AminoLink™ Plus Micro immobilisation kit enables covalent attachment of amine-containing ligands for use in antibody purification and other affinity protocols.

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Monomeric avidin agarose kit, Pierce™

Monomeric avidin agarose kit, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Monomeric Avidin Kit enables mild affinity purification of biotinylated proteins, peptides and other molecules. Avidin monomers have a much lower biotin-binding affinity than native tetrameric avidin enabling dissociation of biotinylated molecules using mild elution conditions.

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Immobilised GST agarose, Pierce™

Immobilised GST agarose, Pierce™

Supplier: Thermo Fisher Scientific

Immobilised GST is glutathione S-transferase from Schistosoma japonicum that has been covalently immobilised on crosslinked 6% beaded agarose.

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High Select™ top14 abundant protein depletion resin, Pierce™

Supplier: Thermo Fisher Scientific

Thermo Scientific™ High Select Top14 Abundant Protein Depletion Resin decreases the abundant protein and antibody components of human plasma samples, helping prepare the samples for mass spectrometry or 1D or 2D electrophoresis.

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Classic IP kit, Pierce™

Classic IP kit, Pierce™

Supplier: Thermo Fisher Scientific

The Pierce™ Classic IP Kit uses protein A/G agarose beads, microcentrifuge spin columns and buffers to perform traditional immunoprecipitation assays.

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Affinity purification kits, Melon™ Gel spin plate kit for IgG screening

Affinity purification kits, Melon™ Gel spin plate kit for IgG screening

Supplier: Thermo Fisher Scientific

The Melon™ Gel Spin Plate Kit for IgG Screening offers a high throughput format for quick purification and screening of antibodies.

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NHS-activated agarose, Pierce™

NHS-activated agarose, Pierce™

Supplier: Thermo Fisher Scientific

NHS-activated agarose is an amine-reactive, beaded-agarose resin for rapid and stable immobilisation of proteins, peptides and other ligands via primary amines.

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c-Myc Tag IP/Co-IP Kit, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ c-Myc Tag IP/Co-IP Kit provides a simple and fast method to study c-Myc-tagged proteins, with advantages over the traditional IP procedure using protein A/G agarose.

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Pierce™ Protein A/G Coated Plate IP Kit

Pierce™ Protein A/G Coated Plate IP Kit

Supplier: Thermo Fisher Scientific

Pierce™ protein A/G coated plate IP kit uses coated protein A/G microplates to perform immunoprecipitation assays in 96-well plates without resins, beads, centrifugation or magnets.

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Affinity chromatography media, high capacity endotoxin removal resin, Pierce™

Affinity chromatography media, high capacity endotoxin removal resin, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ High Capacity Endotoxin Removal Resins are ideal for the removal of endotoxins from protein or antibody samples prior to cell transfection or animal injection.

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Streptavidin HRP conjugates, high sensitivity, Pierce™

Streptavidin HRP conjugates, high sensitivity, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ High Sensitivity Streptavidin-HRP is a peroxidase-conjugated streptavidin biotin-binding protein that provides signal amplification like poly-HRP and exceptional storage stability.

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Immobilisation kit, AminoLink™, Pierce™

Immobilisation kit, AminoLink™, Pierce™

Supplier: Thermo Fisher Scientific

AminoLink™ Immobilisation Kit contains AminoLink™ coupling resin and associated reagents sufficient to perform 5 protein immobilisations, with an expected yield of 2 to 20 mg protein per column.

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Protein stabilising cocktail, Pierce™

Protein stabilising cocktail, Pierce™

Supplier: Thermo Fisher Scientific

The Protein Stabilising Cocktail is a versatile stabilising solution that increases the shelf-life of purified or partially purified proteins during routine storage.

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Inclusion body solubilisation reagent, Pierce™

Inclusion body solubilisation reagent, Pierce™

Supplier: Thermo Fisher Scientific

Inclusion Body Solubilisation Reagent is designed to retrieve expressed protein in soluble form after lysis and extraction with reagents.

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Pierce™ Conjugate Purification Kit

Pierce™ Conjugate Purification Kit

Supplier: Thermo Fisher Scientific

Pierce™ conjugate purification kit is designed to eliminate excess, unconjugated HRP or alkaline phosphatase enzyme from labeled antibodies (IgG) after performing antibody-enzyme conjugation reactions. The single-use column-kit is intended for purification of 0,5 to 50 mg of antibody conjugate dissolved in tris-buffered saline.

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