Hledali jste: Chromatography Columns

Supplier: VARIAN

Bond Elut™ PH is a non-polar bonded silica material which exhibits a different selectivity to alkyl or aliphatic functionalised phases such as C8 or cyclohexyl. The electron density present in the aromatic ring affords an enhancement in the retention of conjugated or aromatic ring-containing analytes due to desirable pi-pi interactions.

Supplier: Agilent

InfinityLab Poroshell 120 HILIC chemistries combine innovative particle technology and bonded phases to improve retention and peak shape for polar analytes. This enables quick and efficient separations of challenging, highly charged polar compounds, and achieves superior analytical sensitivity in MS mode using MS-friendly eluents.

Supplier: VARIAN

Bond Elut™ Jr cartridges have top and bottom Luer fittings allowing easy sample processing when used in conjunction with standard SPE cartridges. Bond Elut™ LRC cartridges have a large reservoir above the sorbent bed and are suitable for use on any standard SPE vacuum manifold.

Supplier: Agilent

Poroshell 120 is made with a superficially porous particle that has a solid silica core and a porous outer layer. This particle provides high efficiency at lower pressures and is ideal for fast or high-resolution separations of many types of analytes.

Supplier: VARIAN

Bond Elut™ C8 is very similar in properties to C18, but is not as retentive for non-polar compounds, due to its shorter hydrocarbon chain, and therefore reduced carbon loading. C8 is an excellent replacement for C18 when analytes are too strongly retained for effective elution. The potential for polar interactions is somewhat higher than for C18 because there is less coverage of the silica surface. These polar interactions are not, however, a significant property of C8.

Supplier: VARIAN

The PLRP-S family of products consists of a range of pore sizes and particle sizes, all with identical chemistry and fundamental adsorptive characteristics. The particles are inherently hydrophobic so there is no bonded phase, alkyl ligand required for reversed-phase separations. This gives a highly reproducible material that is free from silanols and heavy metal ions. Within the extensive product range are columns suitable for analytical separations and preparative purifications.

Supplier: VARIAN

The Bond Elut™ Certify extraction cartridge utilises a packed bed consisting of a special, non-polar C8 sorbent and a strong cation exchanger (SCX). Certify is most commonly used to extract basic (cationic) drugs from urine and blood, but it is also very effective for extraction of a wide range of compounds from a diverse range of aqueous matrices.

Supplier: VARIAN

Bond Elut™ NEXUS is an ultra-clean polymeric sorbent which has bi-modal porosity and a high surface area. NEXUS offers a nonpolar retention mechanism with no pre-conditioning required. The large particle size makes NEXUS ideal for extractions from highly viscous samples, such as horse urine.

Based on the same base polymer technology, Bond Elut™ NEXUS WCX is a weak cation exchange sorbent that offers extra selectivity for analytes, such as quaternary ammonium drugs and anabolic steroids.

Supplier: VARIAN

Bond Elut™ Diol (2OH) resembles unbonded silica in its tendency for strong hydrogen bonding with analytes. 2OH can also be employed in the non-polar mode because the hydrocarbon spacer on its functional group provides enough non-polar character for retention of hydrophobic analytes. Bond Elut™ Diol is a listed SPE device for the DIN 14333-1 method on benzimidazole fungicides.

Supplier: VARIAN

Bond Elut™ PPL is a styrene-divinylvenzene (SDVB) polymer that has been modified with a proprietary non-polar surface. PPL will retain even the most polar classes of analytes, including phenols. The large particle size allows ease of flow for viscous or particulate rich water samples, while the high surface area and strong hydrophobicity ensure reproducible extractions with high recoveries upon elution. Bond Elut™ PPL is suitable for EPA Method 528 ‘Determination of Phenols in Drinking Water by SPE and Capillary GC/MS’.

Supplier: Agilent

Step 2: Dispersive SPE cleanup
Select the dispersive SPE kit suited to the type of food being analysed and the method you are following. In this step, an aliquot of the sample extract from step one (see QuEChERS extraction kits) is added to a 2 or 15 ml centrifuge tube containing a small amount of SPE sorbent and MgSO₄. The sorbent will pull out interfering matrix materials from the sample, while the MgSO₄ helps remove excess water and improve analyte partitioning.

Supplier: Merck

The selectivity offered by ZIC®-HILIC is suitable for a wide variety of molecules containing hydrophilic or ionisable functional groups. This includes compounds such as carbohydrates, metabolites, acids and bases, organic and inorganic ions, metal complexes, amino acids, peptides, protein digests, plant and cell extracts, plus much more. Such compounds are normally characterised by a small or negative LogP value and have poor retention on reversed-phase columns.

Supplier: VARIAN

CP-Al₂O₃/Na₂SO₄ is an aluminum oxide PLOT column that offers high selectivity for separating ppm levels of C₁ to C₅ hydrocarbons in a main stream of C₁ to C₅ hydrocarbons. The Na₂SO₄ deactivation salt provides a polar Al₂O₃ surface, so that unsaturated compounds such as ethylene and acetylene are retained longer. These columns analyse more compounds in a single run than packed columns, while still delivering higher resolution and faster analysis times. When compared to liquid stationary phases, CP-Al₂O₃ provides increased selectivity and allows all C₁ to C₅ hydrocarbon isomers to be separated. CP-Al₂O₃ operates without the need for sub-ambient cooling.

Supplier: Merck

LiChrolut® solid phase extraction (SPE) cartridges and accessories are manufactured to the highest standards. All SPE cartridges listed below are polypropylene unless otherwise stated. They are compatible with all automated SPE instruments and robots. Three important criteria are carefully controlled.

Supplier: VARIAN

Bond Elut™ DEA bears some resemblance to Bond Elut™ NH₂ in its properties but with a slightly lower capacity as an anion exchange sorbent. DEA has a moderately non-polar character due to the alkyl side chains on the amino functionality. These groups still afford a medium level of polarity, higher than C₈ but less polar than C₂ or CN-E.

Supplier: VARIAN

Bond Elut™ Plexa PCX uses a polymeric cation exchange resin that combines the outstanding properties of Bond Elut™ Plexa - superior flow characteristics and improved analytical performance - with strong cation exchange functionalities. This mixed-mode SPE sorbent removes neutral and acidic interferences from the matrix, concentrates basic analytes and therefore improves sensitivity in the determination of basic compounds.

Supplier: VARIAN

PBA is a unique sorbent consisting of a phenylboronic acid covalently linked to a silica gel surface. Covalent processes result in a higher retention to the sorbent surface than other mechanisms, resulting in superior clean-up. The boronate group has a high specificity for cis-diol containing compounds such as catechols, nucleic acids, low molecular weight proteins and carbohydrates.

Supplier: VARIAN

Bond Elut™ PRS is a strong cation exchange sorbent that is also relatively high in polarity. With no appreciable degree of hydrophobicity, in non-polar solvents, PRS is capable of polar and hydrogen bonding interactions. Due to the very low pKa of PRS, a high ionic strength eluent must be used, and so the use of PRS is recommended for weaker cationic species such as pyridinium compounds.

Supplier: Merck

ZIC®-cHILIC is based on the same technology platform as ZIC®-HILIC, but has a different functional group and an improved manufacturing procedure. The different charge orientation of the zwitterionic functional group leads to a different – and complementary – selectivity for polar hydrophilic compounds. This selectivity is especially beneficial for hydrophilic compounds that are negatively charged, positively charged, zwitterionic or neutral, for example, organic acids, amino acids, metabolites, aminoglycosides and nucleotides.

Supplier: VARIAN

Due to the methyl group and subsequent low carbon load, Bond Elut™ C1 is the least retentive of all alkyl group bonded phases for non-polar compounds. However, due to the extensive end capping of this sorbent to mask polar silanol activity, retention and elution of polar and multi-functional analytes can still be achieved. Typical applications include plasma, urine and aqueous samples.

Supplier: VARIAN

The water-wettable, hydroxylated exterior allows excellent flow, even with biological fluids. A gradient of polarity on the polymer surface shunts small analytes to the more hydrophobic centre of the polymer bead, where they are retained prior to the washing and elution steps. Plexa provides these performance enhancements due to a unique polymeric architecture with a non-retentive, hydroxylated, amide-free surface and a non-polar PS/DVB core for retaining small molecules.

Supplier: VARIAN

Bond Elut™ Certify II was developed specifically for the rapid and effective extraction of acidic drugs and metabolites from urine and other biological matrices. Certify II is a mixed-mode cartridge packed with non-polar C8 and strong anion exchange (SAX) sorbent. It has been optimised for acidic drugs such as 11-nor-delta-9-tetrahydrocannibinol-carboxylic acid, salicylic acid, ibuprofen, acetaminophen and other compounds that possess both non-polar and anionic characteristics.

Supplier: VARIAN

Bond Elut™ SCX is a strong cation exchanger with a very low pKa. Although the pKa is similar to Bond Elut™ PRS, the presence of the benzene ring in the functional group increases the potential for non-polar interactions. This non-polar characteristic becomes particularly important when conducting ion-exchange from aqueous systems, where selectivity towards compounds exhibiting cationic and non-polar character is seen.

Supplier: Merck

Chromolith® DIOL columns are useful as an intermediate polarity media between C18 and silica. Diol functionalised media is less polar and can have different selectivity. Chromolith® DIOL can be used with a wide range of solvents in both normal and reverse phases. In normal phase mode, the diol phase provides an alternative selectivity compared to silica whilst additionally demonstrating longer retention times that help to obtain separation. In reverse phase mode, compounds such as water-soluble vitamins, sugars or amino acids are easily baseline separated using simple mobile phases. Chromolith® DIOL columns have long lifetimes within pH range of 2,5 to 7,5, high matrix tolerance and speed of analysis.

Supplier: Agilent

Step 1: Extraction
Choose the extraction salt packet based on your method of analysis, AOAC or EN. The buffered extraction salts are amenable for more liable pesticides. Adding solvent and then salts to a comminuted fruit or vegetable sample (10 g or 15 g) enables you to extract the pesticides of interest into the organic layer. However, adding a food sample with a high percentage of water directly to the salts may create an exothermic reaction that can affect your analyte recoveries.

Supplier: Merck

Purospher® STAR RP-8 endcapped, like Purospher® STAR RP-18 endcapped, is based on high purity silica and has an almost complete surface coverage. Thus, Purospher® STAR RP-8 provides excellent peak symmetry for acidic, basic and even chelating compounds, highest column efficiency in terms of the number of theoretical plates, and exceptional stability from pH 1,5 to 10,5. In addition, they are ideal for a wide range of applications. As the sorbent is less hydrophobic than Purospher® STAR RP-18 endcapped, analytes will typically elute faster on the C8 phase. Purospher® STAR RP-8 endcapped provides enhanced selectivity for positional isomers and symmetrical peak shapes for strongly basic and less hydrophobic compounds.

Supplier: Merck

EXtrelut® NT is a specially processed, inert, wide pore kieselguhr with a high pore volume. It has been used for quite some time within research, for the sample preparation of urine, whole blood, plasma and other biological matrices. Other applications include environmental and residue testing such as analysis of industrial, domestic and wastewater. EXtrelut® NT is supplied either pre-packed in cartridges or as a loose sorbent. EXtrelut® NT simplifies liquid-liquid extraction by replacing separation funnels. Using a single step is more efficient, saves solvent, as well as material and time, in contrast to classic funnel separation. The aqueous sample is simply applied to the EXtrelut® NT sorbent. It distributes itself over the chemically-inert matrix so acting as a stationary phase. Subsequently, elution takes place using organic solvents that are non miscible with water. All the lipophilic substances are extracted from the aqueous into the organic phase.

Supplier: Merck

Purospher® RP-18 is designed for the separation of problematic basic compounds with simple neutral eluents. In addition Purospher® RP-18 allows the separation of hydrophilic compounds with aqueous mobile phase. Purospher® RP-18 is based upon a high purity, metal-free silica. The multi-step chemical modification and deactivation of the surface, and the polar endcapping, enables a peak-tailing-free elution of basic and chelating compounds. Purospher® RP-18 is not suitable for the separation of acidic compounds. The good chemical stability of Purospher® RP-18 enables the use of mobile phase conditions at pH 8 for a long period of time.

Supplier: Merck

Fast method development of complex samples across the pH range from 1,5 to 10,5 with different mobile phases and temperature conditions.
Purospher® STAR RP-18 endcapped HPLC columns are designed for universal use. Basic, neutral and metal-chelating compounds can easily be separated with simple mobile phases – naturally without peak tailing. Thanks to its outstanding performance and stability, Purospher® STAR RP-18 endcapped offers maximum flexibility in method development. Robust methods can be developed over the entire pH range from 1,5 to 10,5. High pH-stability allows the separation of strongly basic compounds with alkaline eluents. The combination of high purity silica, best all-round retention characteristics, outstanding pH stability up to pH 10,5, and suitability for up to 100% aqueous mobile phases, make Purospher® STAR RP-18 endcapped an all-round top performance column, almost universal in its range of applications.

Supplier: SciLabware

These chromatography columns are made from PYREX®, borosilicate glass.