You searched for: Chromatography Columns
Chromatography columns separate molecules within mixtures during gas or liquid methods. Filled with stationary phase compositions or resins, the prepacked options reduce the preparing time required. The vertical pieces are able to withstand extreme temperatures and pressure fluctuations experienced when the mobile phase is forced through the medium. Meet preparative and analytical laboratory procedures with the chromatography columns to separate dissimilar moving components based on individual polarity, composition, and size.
Method development kits
Supplier: Agilent
Agilent offers methods development kits to streamline the column collection process used for medium and high pressure LC systems.
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Solid phase extraction cartridges, Bond Elut™ C2
Supplier: VARIAN
Bond Elut™ C2 is a fairly non-polar sorbent because of the short chain length of the functional group. C2 is often used during the process of method development if analytes are retained too strongly on a C8 or C18 phase. The polarity of C2 is slightly lower than a cyano phase for polar interactions.
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Columns for large biomolecule analysis, CIMmultus™
Supplier: HICHROM
CIMmultus™ columns are based on an innovative monolithic technology. Distinguished by large pores/flow through channels these columns are designed for the production, purification, and analytics of large proteins (IgG, IgM, PEGylated proteins), viruses (AAV, adenovirus, lentivirus, influenza, bacteriophages), VLPs, exosomes and pDNA for example.
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Solid phase extraction cartridges, Bond Elut™ CH
Supplier: VARIAN
Bond Elut™ CH is a mid-polarity sorbent that exhibits unique selectivities for certain analytes. When employed as a non-polar sorbent, CH has the approximate polarity of a C₂ sorbent. Bond Elut™ CH is often a good choice when non-polar sorbents such as C₁₈, C₈ or C₂ do not provide the desired selectivity.
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Solid phase extraction cartridges, Bond Elut™ SI
Supplier: VARIAN
Native silica is generally regarded as the most polar SPE sorbent available. Bond Elut™ SI is particularly effective at separating compounds with a very similar structure. Applying the analytes in a non-polar solvent, then increasing the solvent polarity by increasing the concentration of a polar modifier, such as THF or ethyl acetate, delivers effective separations.
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Solid phase extraction cartridges, Bond Elut™ SAX
Supplier: VARIAN
Bond Elut™ SAX is a strong anion exchange sorbent ideally suited for the extraction of compounds such as carboxylic acids, which may not retain effectively on weak anion exchange sorbents. The sorbent is supplied in the chloride counter ion form. You can modify the selectivity of the phase by replacing the chloride ion with other anions, such as acetate and hydroxide.
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Solid phase extraction cartridges, Bond Elut™ NH₂
Supplier: VARIAN
Bond Elut™ NH₂ is a weaker anion exchanger than sorbents such as SAX (a quaternary amine sorbent that is always charged) and is therefore a better choice for retention of very strong anions, such as sulfonic acids, which may retain irreversibly on a SAX sorbent. Similar to Diol and SI sorbents, Bond Elut™ NH₂ is excellent for the separation of structural isomers.
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Solid phase extraction cartridges, Bond Elut™ C18 OH
Supplier: VARIAN
Bond Elut™ C18 OH is a non-end capped version of the octadecyl bonded phases that enables the silanols on the silica surface to be more active. This low-load C18 has well-controlled silanol activity that permits the fractionation of metabolites and enhances retention of basic compounds compared to an endcapped C18. The pore size makes it a good choice for peptide extraction.
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HPLC columns, preparative scale, Chromolith® Prep
Supplier: Merck
Chromolith® Prep monolithic stationary phases are new ultrapure silica phases which gain their special properties from a bimodal pore structure. This structure is a property of the new 'Sol-Gel' technology and consists of macropores and mesopores. The mesopores with an average diameter of 12 nm, form the fine porous structure of the column interior and create a very large surface area on which adsorption of the target compounds occurs.
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Chromolith® NH₂ HPLC Columns
Supplier: Merck
Made of highly porous monolithic rods of silica with a revolutionary bimodal pore structure, therefore, high separation efficiencies are reached under low back pressures. These columns have long lifetimes within pH range of 2,5 to 7,5, high matrix tolerance and speed of analysis. Technical information.
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Solid phase extraction cartridges, Bond Elut™ AccuCAT
Supplier: VARIAN
Bond Elut AccuCAT cartridges are mixed bed SPE cartridges consisting of a strong cation exchange (SCX) and a strong anion exchange (SAX) sorbent packed into one bed. AccuCAT is effective for the extraction of acidic, basic and neutral analytes from urine and other biological samples. AccuCAT is particularly effective for catecholamine extraction from bio fluids.
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SampliQ SPE cartridges
Supplier: Agilent
High quality SampliQ SPE (Solid Phase Extraction) products help the user confidently extract and concentrate samples from complex matrices, ensuring fast, accurate, and reproducible results from the very first step. The SampliQ line includes polymers, silica and non-silica options to meet user's specific SPE needs.
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C18 SPE cartridges, Bond Elut™
Supplier: Agilent
Bond Elut™ C18 is a hydrophobic silica sorbent. C18 is commonly used for non-polar compounds. Bond Elut™ C18 is endcapped to reduce interactions with silanol groups.
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Solid phase extraction cartridges, Bond Elut™ C8
Supplier: VARIAN
Bond Elut™ C8 is very similar in properties to C18, but is not as retentive for non-polar compounds, due to its shorter hydrocarbon chain, and therefore reduced carbon loading. C8 is an excellent replacement for C18 when analytes are too strongly retained for effective elution. The potential for polar interactions is somewhat higher than for C18 because there is less coverage of the silica surface. These polar interactions are not, however, a significant property of C8.
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Chiral columns, ChiraDex®
Supplier: Merck
ChiraDex® is a versatile HPLC column characterised by broad enantioselectivity and can be used for the separation of enantiomers of numerous different classes of substances. ChiraDex® is based on beta-cyclodextrin covalently linked to spherical particles of silica, and is ideal for chiral separation of hydrocarbons, steroids, phenol esters and derivatives, aromatic amines, heterocycles with five- to seven-membered ring. Simply composed RP-eluents can be used in most separations.
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HPLC columns, SUPELCOSIL™, Suplex™ pKb-100, Supelco®
Supplier: Merck
Suplex pKb-100 columns feature the same bonded phase functionality as SUPELCOSIL LC-ABZ columns. These specially deactivated columns differ in that Suplex pKb-100 is not endcapped, while SUPELCOSIL LC-ABZ is endcapped. The absence of the end-capping reagent results in better performance from Suplex pKb-100 for the strongest basic compounds, while LC-ABZ is preferred when the sample also contains acids and/or zwitterions.
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Solid phase extraction cartridges, Bond Elut™ PH
Supplier: VARIAN
Bond Elut™ PH is a non-polar bonded silica material which exhibits a different selectivity to alkyl or aliphatic functionalised phases such as C8 or cyclohexyl. The electron density present in the aromatic ring affords an enhancement in the retention of conjugated or aromatic ring-containing analytes due to desirable pi-pi interactions.
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Analytical HPLC columns, Microsorb™ C18
Supplier: VARIAN
Microsorb™ C18 is an octadecylsilane-covered silica that has been bonded using monofunctional bonding agents under optimised conditions. This material is then endcapped with trimethylsilyl groups to minimise nonspecific binding, maximise mass recoveries and improve peak shape.
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Solid phase extraction cartridges, Bond Elut™ CN-U
Supplier: VARIAN
Bond Elut CN-U is ideally suited for the extraction of polar compounds from a nonpolar matrix, such as hexane or oils. It is suitable for very polar analytes that may be irreversibly retained on SI or Diol (2OH) SPE phases.
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HPLC columns, MetaSil AQ C18
Supplier: VARIAN
The MetaSil AQ materials are based on silica that is highly coated with hydrophobic alkyl chains interspersed with hydrophilic short-chain endcapping groups. These columns remain fully extended in aqueous phases, allowing increased interaction between samples and the bonded phase. These columns remain fully extended in aqueous phases, allowing increased interaction between samples and the bonded phase.
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Solid phase extraction cartridges, Bond Elut™ LMS
Supplier: VARIAN
Bond Elut™ LMS polymeric sorbent lets you elute without having to add amine modifiers, buffers, or acids. The elimination of secondary interactions means that elution of analytes can be achieved with pure organic solvents or HPLC mobile phase-compatible solvent mixtures of low ionic strength. These characteristics are crucial to allow compatibility with LC/MS or other delicate analytical techniques.
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Solid phase extraction cartridges, EnvirElut™
Supplier: VARIAN
EnvirElut™ sorbents are specially designed for the extraction of a wide range of compounds from aqueous matrices. EnvirElut™ PAH and Pesticides are available in standard SPE straight barrel cartridges, which can be used on conventional vacuum manifolds such as the Vac Elut SPS 24.
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HPLC columns, Chromolith® CN
Supplier: Merck
Cyano columns are generally more polar than traditional alkyl silica columns. The functional groups are highly ordered, reducing steric hindrance for the solute. The modification also allows cation exchange activity, which is higher at neutral pH than in acidic conditions. Chromolith® CN columns are suitable for the separation of alkaloids, oils, flavonoids, glycols, phenols, phthalates, steroids and sulfonamides.
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UHPLC columns, Purospher® STAR RP-18 endcapped
Supplier: Merck
Available with optimised 2 μm particulate silica, these UHPLC columns, based on Purospher® STAR RP-18 endcapped, are ideal for ultra-fast applications, where resolution, sensitivity and sample throughput are crucial. They are the first choice for high throughput screening and QC analyses, process monitoring, method development and LC-MS applications. Due to its balanced selectivity, Purospher® STAR RP-18 endcapped UHPLC columns cover almost all demanding separations with tailing-free chromatograms. Purospher® STAR RP-18 endcapped 3 μm columns are recommended for difficult samples where clogging and back pressure present an issue.
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HPLC columns, Chromolith®
Supplier: Merck
Monolithic silica technology: A new age in chromatography. Chromolith® columns are based on silica so existing methods can be easily transferred with only minimal investment in new method development work. 'Sol-Gel' technology allows highly porous monolithic rods of silica to be formed. These feature a bimodal pore structure provides a unique combination of macropores and mesopores.
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High temperature columns, VF-5ht and VF-5ht Ultimetal
Supplier: VARIAN
The VF-5ht improves the analysis of high boiling compounds by exhibiting low bleed, even at high temperatures. Based on ultra-low bleed FactorFour technology, VF-5ht provides unmatched selectivity, sensitivity, and accuracy for the analysis of high-molecular weight compounds.
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HPLC columns, Poroshell 120 EC-C18
Supplier: Agilent
Poroshell 120 is made with a superficially porous particle that has a solid silica core and a porous outer layer. This particle provides high efficiency at lower pressures and is ideal for fast or high-resolution separations of many types of analytes.
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HPLC columns, Poroshell 120 SB-C18
Supplier: Agilent
Poroshell 120 is made with a superficially porous particle that has a solid silica core and a porous outer layer. This particle provides high efficiency at lower pressures and is ideal for fast or high-resolution separations of many types of analytes.
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Solid phase extraction cartridges, Bond Elut™ Certify
Supplier: VARIAN
The Bond Elut™ Certify extraction cartridge utilises a packed bed consisting of a special, non-polar C8 sorbent and a strong cation exchanger (SCX). Certify is most commonly used to extract basic (cationic) drugs from urine and blood, but it is also very effective for extraction of a wide range of compounds from a diverse range of aqueous matrices.
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Solid phase extraction cartridges, Bond Elut™ Diol
Supplier: VARIAN
Bond Elut™ Diol (2OH) resembles unbonded silica in its tendency for strong hydrogen bonding with analytes. 2OH can also be employed in the non-polar mode because the hydrocarbon spacer on its functional group provides enough non-polar character for retention of hydrophobic analytes. Bond Elut™ Diol is a listed SPE device for the DIN 14333-1 method on benzimidazole fungicides.