Hledali jste: X-Gal

Supplier: VWR Peqlab

Substrate for the detection of beta galactosidase.

Supplier: VWR Chemicals

X-Gal is a chromogenic substrate for ß-Galactosidase that forms an intense blue precipitate. Used in conjunction with IPTG in a colorimetric assay, X-Gal allows differentiation of recombinants from non recombinants in cloning experiments with vectors containing the lacZ gene. It is also used in microbiology to detect microorganisms which have ß-Galactosidase activity.

Supplier: VWR Chemicals

X-Gal ≥99% (by enzymatic purity), Ultra Pure Grade

Supplier: Apollo Scientific

X-Gal, a chromogenic substrate of beta-Galactosidase. Used in conjunction with IPTG for detection of beta-Galactosidase activity in bacterial colonies in a colorimetric assay.

Supplier: Thermo Fisher Scientific

X-Gal 99%, pure

Supplier: PanReac AppliChem

X-Gal

Supplier: PanReac AppliChem

X-Gal

Supplier: Rockland Immunochemicals

X-Gal

Supplier: ENZO LIFE SCIENCES

X-Gal

Supplier: Thermo Fisher Scientific

Thermo Scientific X-Gal (5-bromo-4-chloro-3-indolyl-beta-D-galacto-pyranoside) is an inert chromogenic substrate for beta-galactosidase which hydrolyzes X-Gal into colorless galactose and 4-chloro-3-brom-indigo, forming an intense blue precipitate

Supplier: Thermo Fisher Scientific

X-Gal 99+% for molecular biology DNase-, RNase-, protease-free

Supplier: OZ BIOSCIENCES

X-Gal substrate is metabolised by the b-galactosidase enzyme. The quality and purity of the substrate is essential to obtain high-quality and reliable results.

Supplier: EDVOTEK

X-Gal

Supplier: OZ BIOSCIENCES

X-Gal staining kit allows to visualise ß-gal expression through hydrolysis of the X-Gal (5-bromo-4-chloro-3-indoyl-beta-D-Galactopyranoside) substrate yielding blue precipitates.

Supplier: Corning

X-Gal (5-bromo-4-chloro-3-indolyl-b-D-galactopyranoside) is a substrate of b-galactosidase, a gene product of the lac operon in E. coli.

Supplier: G-Biosciences

X-Gal

Supplier: G-Biosciences

X-gal (5-bromo-4-chloro-3-indolyl-β-D- galactopyranoside) is used to indicate whether bacteria express the ß-galactosidase enzyme, which is encoded by the lacZ gene. X-gal is cleaved by ß-galactosidase yielding galactose and 5-bromo-4-chloro-3-hydroxyindole, which is oxidised into 5,5'-dibromo-4,4'-dichloro-indigo, an insoluble blue product. Thus, if X-gal and an inducer of ß-galactosidase (usually IPTG) is contained within an agar medium on a culture plate, colonies which have a functional lacZ gene can easily be distinguished by their blue colour.

Supplier: Thermo Fisher Scientific

X-Gal ≥98%

Supplier: Biotium

X-Gal

Supplier: MP Biomedicals

Storage: Store at -20 °C. Store Desiccated. Store Under Nitrogen. Protect from light.
5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside, commonly known as X−Gal, is a histochemical substrate for β−galactosidase.
5-Bromo-4-Chloro-3-Indolyl-β-D-Galactopyranoside is used as indigogenic substrate for β-galactosidase, for detection of β-galactosidase-positive clones, and the identification of lac and bacterial colonies or phage plaques. It is the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac+ genotype. X−Gal is cleaved by β−galactosidase to yield an insoluble blue precipitate. X−Gal is particularly useful in molecular biology applications to detect the activity of β−galactosidase which is frequently used as a reporter gene. In cloning, X−Gal is used to detect insertion of foreign DNA into the lacZ region of plasmid DNA using α-complementation which is based on vectors such as the pUC and the M13mp series that carry a fragment of the β-galactosidase gene encoding an α-fragment of β-galactosidase.

Supplier: MP Biomedicals

5-Bromo-4-chloro-3-indolyl b-D-galactopyranoside, commonly known as X-Gal, is a histochemical substrate for b-galactosidase. X-Gal is cleaved by b-galactosidase to yield an insoluble blue precipitate.

Supplier: Merck

X-Gal, Sigma-Aldrich®