You searched for: Enzyme Assays

Either measuring substrate consumption or overall production, the enzyme assays are used to monitor enzymatic activity. Provided with the required supplies and reagents for any preferred method, enzyme kinetics and inhibition studies can be successfully completed. While continuous assay sampling methods give activity readings throughout development, discontinuous techniques calculate after the reaction has been stopped. The standardized enzyme assays will provide accurate identification and measurement of the protein molecules present in samples.

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Amplite® Colorimetric α-Ketoglutarate Quantitation Kit

Supplier: AAT BIOQUEST

Alpha-ketoglutarate (α-Ketoglutarate) is a key molecule in the Krebs cycle determining the overall rate of the citric acid cycle of the organism.

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Amplite® Fluorimetric Zinc Ion Quantitation Kit

Supplier: AAT BIOQUEST

Zinc is an essential trace mineral element that plays an important role in a number of biological processes.

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Amplite® Colorimetric Glucose and Sucrose Assay Kit

Supplier: AAT BIOQUEST

Designed for consistent and dependable results. The Amplite® Colorimetric Glucose and Sucrose Assay Kit offers a convenient method for measuring glucose and sucrose levels in various biological samples, such as serum, plasma, body fluids, food, tissue extracts, and growth medium.

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Amplite® Fluorimetric Total Thiol Quantitation Assay Kit Green Fluorescence

Supplier: AAT BIOQUEST

The detection and measurement of free thiol (such as free cysteine, glutathione and cysteine residues in proteins) is one of the essential tasks for investigating biological processes and events in many biological systems.

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Amplite® Rapid Fluorimetric Total Thiol Quantitation Assay Kit Green Fluorescence

Supplier: AAT BIOQUEST

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Amplite® Fluorimetric Coenzyme A Quantitation Kit Green Fluorescence

Supplier: AAT BIOQUEST

Coenzyme A (CoA) is a universal and essential cofactor in all forms of cellular life acting as a principal acyl carrier in numerous biosynthetic, energy-yielding, and degradative pathways.

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β-Galactosidase Assay Kit and Reagents

Supplier: Thermo Fisher Scientific

The Mammalian β-Galactosidase Assay Reagent Kit is a simple and versatile assay to measure β-galactosidase activity in cultured mammalian cells. Cells grown on a plate may be lysed and tested for β-galactosidase activity by adding a single all-in-one lysis-and-assay solution. Alternatively, M-PER™ Mammalian Protein Extraction Reagent may be used to prepare lysate from washed adherent or pelleted cells, and then a portion of the lysate used to assay for β-galactosidase activity. With M-PER Reagent, there is no need to scrape cells or to perform freeze-thaw cycles. The remaining lysates prepared with M-PER™ Reagent may be used for applications such as reporter assays (luciferase, β-galactosidase and chloramphenical acetyltransferase), protein kinase assays (PKA, PKC, and tyrosine kinase), immunoassays (Western blot, ELISA, RIA) and protein purification.

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Amplite® Fluorimetric L-Lactate Dehydrogenase (LDH) Assay Kit

Supplier: AAT BIOQUEST

Lactate dehydrogenase (LDH) is an oxidoreductase enzyme that catalyses the interconversion of pyruvate and lactate.

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Amplite® Fluorimetric Alkaline Phosphatase Assay Kit Blue Fluorescence

Supplier: AAT BIOQUEST

Alkaline phosphatase is a highly sensitive enzyme for ELISA, immuno-histochemical, Northern, Southern and Western blot applications.

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Amplite® Fluorimetric Glycerol Assay Kit

Supplier: AAT BIOQUEST

Glycerol is a precursor for the synthesis of triglycerides and phospholipids in liver and adipose tissue.

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Amplite® Fluorimetric Glucose-6-Phosphate Assay Kit

Supplier: AAT BIOQUEST

Glucose-6-phosphate (G6P) is a key intermediate for glucose transport into cells.

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Amplite® Renilla Luciferase Reporter Gene Assay Kit Bright Glow

Supplier: AAT BIOQUEST

Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase.

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Amplite® Gaussia Luciferase Reporter Gene Assay Kit Bright Glow

Supplier: AAT BIOQUEST

Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase.

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Amplite® Fluorimetric Lipase Activity Assay Kit

Supplier: AAT BIOQUEST

Designed for consistent and dependable results. The Amplite® Fluorimetric Lipase Activity Assay Kit provides a simple and quick protocol for measuring lipase activity.

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Amplite® Cholesterol Quantitation Kit

Supplier: AAT BIOQUEST

Cholesterol is required to build and maintain cell membranes.

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Amplite® Colorimetric NADPH Assay Kit

Supplier: AAT BIOQUEST

Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells.

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MMP Green™ Substrate

Supplier: AAT BIOQUEST

The matrix metalloproteinases (MMPs) constitute a family of zinc-dependent endopeptidases that function within the extracellular matrix.

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Amplite® Rapid Colorimetric Total Protein Thiol Quantitation Assay Kit

Supplier: AAT BIOQUEST

Protein thiols are very important to protein structure, protein function and biological system redox environment.

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Amplite® Colorimetric Hexokinase Assay Kit

Supplier: AAT BIOQUEST

Designed for consistent and dependable results. The Amplite® Colorimetric Hexokinase Assay Kit is designed to accurately measure the activity of hexokinases from various sources using a simple and quick protocol.

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Amplite® Colorimetric Hydrogen Peroxide Assay Kit

Supplier: AAT BIOQUEST

Hydrogen peroxide (H2O2) is a reactive oxygen metabolic by-product that serves as a key regulator for a number of oxidative stress-related states.

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Amplite® Fluorimetric Total NADP and NADPH Assay Kit Red Fluorescence

Supplier: AAT BIOQUEST

Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells.

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Amplite® Fluorimetric Goat Anti-Rabbit IgG-HRP Conjugate ELISA Assay Kit Red Fluorescence

Supplier: AAT BIOQUEST

Horseradish Peroxidase (HRP) is a small molecule (MW ~40 KD) that is widely used in a variety of biological detections.

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Gaussia-firefly luciferase dual assay kit, Pierce™, Thermo Scientific

Supplier: Thermo Fisher Scientific

The Thermo Scientific Pierce Gaussia-Firefly Luciferase dual assay kit provides the necessary reagents to simultaneously detect intracellular Gaussia and red firefly luciferase activity in mammalian whole cell lysates.

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SAM Methyltransferase assay, SAM510™

Supplier: G-Biosciences

SAM510™ assay is a continuous enzyme coupled assay that can continuously monitor SAM-dependent methyltransferases without the use of radioactive labels or endpoint measurements.

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Fluorescent β-Galactosidase assay kit

Supplier: G-Biosciences

The Fluorescent β-Galactosidase assay is used for determining the β-galactosidase activity in the lysates of cells transfected with a β-galactosidase expression construct. The Fluorescent β-Galactosidase assay (MUG) provides a 96-well assay format for galactosidase activity that is suitable for high throughput applications. The production of the fluorphore is monitored at an emission/excitation wavelength of 365/460 nm.

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Kinase enrichment kits, Pierce™

Supplier: Thermo Fisher Scientific

The Pierce™ Kinase Enrichment Kits utilise an ActivX™ ATP or ADP probe to covalently label the active site of ATPases, including chaperones and metabolic enzymes, to enable their selective enrichment using a desthiobiotin tag.

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Protease Screening Kit

Supplier: G-Biosciences

The Protease Screening Kit provides a simple and quick method for testing samples for proteolysis. Simply incubate sample in the reagent provided and obtain results.

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Active GTPase pull-down and detection kits

Supplier: Thermo Fisher Scientific

The Active GTPase Pull-Down and Detection Kits allows the monitoring of changes in GTPase activity levels by enriching and detecting the active (GTP-bound) state of a particular GTPase.

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Fluorescent β-Galactosidase assay kit

Supplier: G-Biosciences

β-Galactosidase assay is for determining the β-galactosidase activity in the lysates of cells transfected with a β-galactosidase expression construct. It uses chlorophenol red-β-D-galactopyranoside (CPRG), which is up to 10 times more sensitive than the classic O-nitrophenyl-β-D-galactopyranoside (ONPG) β-Galactosidase assays. This increased sensitivity makes it simpler to measure β-galactosidase activity in cells that are difficult to transfect or have low β-galactosidase activity expression. β-Galactosidase activity is measured using a spectrophotometer or a microplate reader to determine the amount of substrate converted at 570 to 595 nm.