You searched for: Enzyme Assays
Either measuring substrate consumption or overall production, the enzyme assays are used to monitor enzymatic activity. Provided with the required supplies and reagents for any preferred method, enzyme kinetics and inhibition studies can be successfully completed. While continuous assay sampling methods give activity readings throughout development, discontinuous techniques calculate after the reaction has been stopped. The standardized enzyme assays will provide accurate identification and measurement of the protein molecules present in samples.
Amplite® Colorimetric Tyrosinase Assay Kit
Supplier: AAT Bioquest
Tyrosinase is of great interest to drug discovery, life science research, food industry and cosmetics industry since it plays an important role in the biosynthetic pathway of melanin.
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Amplite® Fluorimetric NADP/NADPH Ratio Assay Kit *Red Fluorescence*
Supplier: AAT Bioquest
Nicotinamide adenine dinucleotide (NAD⁺) and nicotinamide adenine dinucleotide phosphate (NADP⁺) are two important cofactors found in cells.
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Amplite® IR
Supplier: AAT Bioquest
Our Amplite® IR is a fluorogenic peroxidase substrate that generates near infrared fluorescence upon reaction with peroxidase and H2O2.
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Amplite® Fluorimetric Malondialdehyde (MDA) Quantitation Kit
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Malondialdehyde (MDA) is one of the natural byproducts during lipid peroxidation.
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Amplite® Fluorimetric Glucose Oxidase Assay Kit *Red Fluorescence*
Supplier: AAT Bioquest
The glucose oxidase is a dimeric protein that catalyses the oxidation of beta-D-glucose into hydrogen peroxide and D-glucono-1,5-lactone, which is hydrolyzed to gluconic acid.
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Amplite® Colorimetric Sphingomyelinase Assay Kit *Blue Color*
Supplier: AAT Bioquest
Sphingomyelinase (SMase) is an enzyme that is responsible for cleaving sphingomyelin (SM) to phosphocholine and ceramide.
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Amplite® Colorimetric Endotoxin Detection Kit
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Lipopolysaccharide (LPS), also known as endotoxin, is the major component of the outer membranes of Gram-negative bacteria.
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Amplite® Fluorimetric Glucose and Sucrose Assay Kit
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Designed for consistent and dependable results. The Amplite® fluorimetric glucose and sucrose assay kit offers a convenient method for measuring glucose and sucrose levels in various biological samples, such as serum, plasma, body fluids, food, tissue extracts, and growth medium.
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Amplite® Luminometric Alkaline Phosphatase Assay Kit *Luminescence*
Supplier: AAT Bioquest
Alkaline phosphatase is a highly sensitive enzyme for ELISA, immuno-histochemical, Northern, Southern and Western blot applications.
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Amplite® Colorimetric Acetylcholinesterase Assay Kit
Supplier: AAT Bioquest
Acetylcholinesterase, also known as AChE, is an enzyme that degrades (through its hydrolytic activity) the neurotransmitter acetylcholine, producing choline and an acetate group.
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ReadiUse™ NADPH Regenerating Kit
Supplier: AAT Bioquest
NADPH provides the reducing equivalents for biosynthetic reactions and for oxidation-reduction involved in protection against the toxicity of ROS (reactive oxygen species).
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Amplite® Colorimetric Beta-Lactamase Activity Assay Kit
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β-lactamases are a large family of enzymes capable of hydrolysing β-lactams.
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Amplite® Fluorimetric Glutathione Assay Kit *Green Fluorescence*
Supplier: AAT Bioquest
The monitoring of reduced and oxidised glutathione (GSH) in biological samples is essential for evaluating the redox and detoxification status of cells and tissues in relation to the protective role of glutathione against oxidative and free-radical-mediated cell injury.
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Amplite® Fluorimetric Acetylcholinesterase Assay Kit *Red Fluorescence*
Supplier: AAT Bioquest
Acetylcholinesterase, also known as AChE, is an enzyme that degrades (through its hydrolytic activity) the neurotransmitter acetylcholine, producing choline and an acetate group.
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Amplite® Colorimetric Bradford Protein Quantitation Assay Kit
Supplier: AAT Bioquest
The traditional Bradford protein assay is widely used for quantifying protein concentrations.
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Amplite® Fluorimetric NAD Assay Kit *Blue Fluorescence*
Supplier: AAT Bioquest
Nicotinamide adenine dinucleotide (NADH) and its oxidized form (NAD) are essential cofactors for many enzyme reactions found in living cells.
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Amplite® Fluorimetric D-Lactate Dehydrogenase (LDH) Assay Kit
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Lactate dehydrogenase (LDH) is an oxidoreductase enzyme that catalyses the interconversion of pyruvate and lactate.
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Amplite® Fluorimetric L-Alanine Assay Kit
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L-alanine (L-Ala) plays a crucial role as a building block of important proteins.
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Amplite® Fluorimetric Beta-Galactosidase Assay Kit *Green Fluorescence*
Supplier: AAT Bioquest
E. coli beta-galactosidase is a 464 Kd tetramer.
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Amplite® Fluorimetric Renin Assay Kit *Red Fluorescence*
Supplier: AAT Bioquest
Renin is an enzyme that participates in the body's renin-angiotensin system (RAS) that mediates extracellular volume and arterial vasoconstriction.
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Amplite® Fluorimetric Glutathione Peroxidase Assay Kit *Blue Fluorescence*
Supplier: AAT Bioquest
Glutathione peroxidase is an enzyme family with peroxidase activity to protect the organism from oxidative damage.
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Amplite® Fluorimetric Neuraminidase Assay Kit *Blue Fluorescence*
Supplier: AAT Bioquest
Neuraminidases, also called sialidases, are glycoside hydrolase enzymes that catalyze the hydrolysis of terminal sialic acid residues and neuraminic acid.
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Amplite® Colorimetric Calcium Quantitation Kit *Blue Color*
Supplier: AAT Bioquest
Calcium is essential for all living organisms, particularly in cell physiology, where movement of the calcium ion Ca²⁺ into and out of the cytoplasm functions as a signal for many cellular processes.
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Amplite® Fluorimetric Lysyl Oxidase Assay Kit *Red Fluorescence*
Supplier: AAT Bioquest
Lysyl oxidase (LOX) is an extracellular enzyme that catalyses formation of aldehydes from lysine residues in collagen and elastin precursors.
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Amplite® Colorimetric Enterokinase Activity Assay Kit
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Enterokinase (also called enteropeptidase) is a serine protease produced by cells in the duodenal wall and is a key enzyme in human and animal digestion system.
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Amplite® Colorimetric Pyruvate Assay Kit
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Pyruvate is an important chemical compound in intracellular metabolic pathways.
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Amplite® Colorimetric Maleimide Quantitation Kit
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Maleimides can be directly assayed spectrophotometrically at 302 nm.
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Amplite® Universal Fluorimetric Protease Activity Assay Kit *Green Fluorescence*
Supplier: AAT Bioquest
Monitoring of various protease activities has become a routine task for many biological laboratories.
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Amplite® Colorimetric L-Alanine Assay Kit
Supplier: AAT Bioquest
L-alanine (L-Ala) plays a crucial role as a building block of important proteins.
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Beadlite™ Rapid Colorimetric Thiol Quantitation Kit for Nanoparticles
Supplier: AAT Bioquest
Designed for consistent and dependable results. Beadlite™ Rapid Colorimetric Thiol Quantitation kit provides a robust method to quantify thiol-functionalised nanoparticle with our proprietary Thiolite™ 490 that has its maximum absorbance at ~490 nm.