You searched for: Enzyme Assays
Either measuring substrate consumption or overall production, the enzyme assays are used to monitor enzymatic activity. Provided with the required supplies and reagents for any preferred method, enzyme kinetics and inhibition studies can be successfully completed. While continuous assay sampling methods give activity readings throughout development, discontinuous techniques calculate after the reaction has been stopped. The standardized enzyme assays will provide accurate identification and measurement of the protein molecules present in samples.
Amplite® Colorimetric Glucose-6-Phosphate Dehydrogenase (G6PD) Assay Kit
Supplier: AAT BIOQUEST
Glucose-6-phosphate dehydrogenase (G6PD) catalyses the conversion of glucose-6-phosphate to 6-phosphoglucono-δ-lactone, the first and rate-limiting step in the pentose phosphate pathway.
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Renilla luciferase glow assay kits, Pierce™
Supplier: Thermo Fisher Scientific
The Thermo Scientific Pierce renilla Luciferase glow assay kit provides an extremely bright and stable bioluminescence signal (half-life approx. 3 hours), especially in the presence of green renilla luciferase reporter.
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Renilla-firefly luciferase dual assay kit, Pierce™, Thermo Scientific
Supplier: Thermo Fisher Scientific
The Thermo Scientific Pierce Renilla-Firefly Luciferase dual assay kit provides the necessary reagents to simultaneously detect intracellular Renilla and Red Firefly luciferase activity in mammalian whole cell lysates.
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Amplite® Colorimetric Biotin Quantitation Kit
Supplier: AAT BIOQUEST
The avidin/streptavidin-biotin interaction is the strongest known non-covalent biological interaction (Kd = 10 to 15 M⁻¹) between a protein and its ligand.
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Amplite® Colorimetric Malondialdehyde (MDA) Quantitation Kit
Supplier: AAT BIOQUEST
Malondialdehyde (MDA) is natural byproduct of lipid peroxidation and is widely used as a key indicator to determine the oxidative stress and free radical formation.
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Amplite® Rapid Colorimetric APC-Maleimide Quantitation Kit
Supplier: AAT BIOQUEST
Designed for consistent and dependable results. The Amplite® Rapid Colorimetric APC-Maleimide Quantitation Kit facilitates the quantification of maleimide groups on maleimide-activated APC proteins using a proprietary sensor, Maleimide 555™, which has an absorption peak at ~555 nm.
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Amplite® Fluorimetric Tyrosinase Assay Kit
Supplier: AAT BIOQUEST
Tyrosinase is an enzyme expressed across a vast range of species from bacteria and fungi to animals.
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Amplite® Colorimetric L-Lactate Dehydrogenase (LDH) Assay Kit
Supplier: AAT BIOQUEST
Lactate dehydrogenase (LDH) is an oxidoreductase enzyme that catalyses the interconversion of pyruvate and lactate.
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ONPG assay kit
Supplier: OZ BIOSCIENCES
ONPG assay kit is ideal to quantify high expression level of beta-Gal.
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Amplite® Fluorimetric Pyruvate Assay Kit
Supplier: AAT BIOQUEST
Pyruvate is an important chemical compound in intracellular metabolic pathways.
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Amplite® Fluorimetric Acetylcholinesterase Assay Kit *Green Fluorescence*
Supplier: AAT BIOQUEST
Acetylcholinesterase, also known as AChE, is an enzyme that degrades (through its hydrolytic activity) the neurotransmitter acetylcholine, producing choline and an acetate group.
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Amplite® Fluorimetric Xanthine Oxidase Assay Kit *Red Fluorescence*
Supplier: AAT BIOQUEST
Xanthine oxidase (XO) is an enzyme that catalyses the oxidation of hypoxanthine to xanthine and can further catalyze the oxidation of xanthine to uric acid.
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Amplite® Fluorimetric Glycerol 3-Phosphate (G3P) Assay Kit
Supplier: AAT BIOQUEST
Glycerol 3-Phosphate (G3P) is an important intermediate in the glycolysis metabolic pathway.
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Amplite® Fluorimetric Myeloperoxidase Assay Kit *Red Fluorescence*
Supplier: AAT BIOQUEST
Myeloperoxidase (MPO), most abundantly present in neutrophils and monocytes, is a green hemoprotein having peroxidase activity.
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Amplite® Fluorimetric Monoamine Oxidase Assay Kit *Red Fluorescence*
Supplier: AAT BIOQUEST
Monoamine oxidases (MAO) are a family of flavin-containing amine oxidoreductases that catalyse the oxidation of monoamines.
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Amplite® Colorimetric Peroxidase (HRP) Assay Kit *Blue Color*
Supplier: AAT BIOQUEST
Peroxidase is a small molecule (MW ~40 KD) that can usually be conjugated to an antibody in a 4:1 ratio.
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Amplite® Fluorimetric Sphingomyelinase Assay Kit *Red Fluorescence*
Supplier: AAT BIOQUEST
Five types of sphingomyelinase (SMase) have been identified based on their cation dependence and pH optima of action.
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Amplite® Fluorimetric NADH Assay Kit *Red Fluorescence*
Supplier: AAT BIOQUEST
Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells.
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MMP-3 Green™ Substrate
Supplier: AAT BIOQUEST
The matrix metalloproteinases (MMPs) constitute a family of zinc-dependent endopeptidases that function within the extracellular matrix.
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Pierce™ Gaussia Luciferase Flash Assay Kit
Supplier: Thermo Fisher Scientific
The Thermo Scientific Pierce Gaussia Luciferase Flash Assay Kit provides researchers with the most sensitive assay for transcriptional activity of regulatory elements in mammalian cell culture media and whole cell lysate.
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Amplite® Colorimetric Asparaginase Activity Assay Kit
Supplier: AAT BIOQUEST
Designed for consistent and dependable results. The Amplite® colorimetric asparaginase activity assay kit provides a simple and straightforward procedure for measuring asparaginase activity in a variety of biological samples.
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Amplite® Fluorimetric Proteasome 20S Activity Assay Kit *Green Fluorescence*
Supplier: AAT BIOQUEST
Our Amplite® Fluorometric 20S Proteasome Assay Kit is a homogeneous fluorescent assay that measures the chymotrypsin-like protease activity associated with the proteasome complex either in cultured cells or cell lysates.
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Amplite® Fluorimetric Glucose-6-Phosphate Dehydrogenase (G6PD) Assay Kit
Supplier: AAT BIOQUEST
Glucose-6-phosphate dehydrogenase (G6PD) catalyses the conversion of glucose-6-phosphate to 6-phosphoglucono-δ-lactone, the first and rate-limiting step in the pentose phosphate pathway.
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Amplite® Red Peroxidase Substrate
Supplier: AAT BIOQUEST
Amplite® Red is a sensitive fluorogenic peroxidase substrate that generates a highly red fluorescent product that has maximum absorption of 571 nm and maximum emission of 585 nm.
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Amplite® Ethanol Quantitation Kit
Supplier: AAT BIOQUEST
The ability to rapidly perform quantitative measurements of ethanol is highly desirable in life science research, clinical evaluations, and food and drug industries.
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Amplite® Fluorimetric α-Ketoglutarate Quantitation Kit
Supplier: AAT BIOQUEST
Alpha-ketoglutarate (α-ketoglutarate) is a key molecule in the Krebs cycle determining the overall rate of the citric acid cycle of the organism.
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Peroxide assay kit, PEROXsay™
Supplier: G-Biosciences
PEROXsay™ is a colorimetric quantitative peroxide assay that measures the oxidation of ferrous (Fe²⁺+) ions to ferric (Fe³⁺) ions. Peroxides react with a sugar alcohol converting it to a peroxyl radical that subsequently starts the oxidation of ferrous ions to ferric ions. The acidic pH of the assay allows the ferric (Fe³⁺) ion to complex with the colorimetric reagent, resulting in a change in absorbance that is proportional to the peroxide concentration.
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CPRG assay kit
Supplier: OZ BIOSCIENCES
CPRG assay kit is designed to quantify low expression level β-Galactosidase.
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Amplite® Fluorimetric Protein Quantitation Kit *Orange Fluorescence*
Supplier: AAT BIOQUEST
Protein quantification is an essential task in protein purification, electrophoresis, cell biology, molecular biology and other research applications.
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Amplite® Fluorimetric Aldehyde Quantitation Kit
Supplier: AAT BIOQUEST
The formation, reactivity and toxicity of aldehydes originating from the peroxidation of lipids of cellular membranes have received great attention in recent years.