You searched for: Enzyme Assays
Either measuring substrate consumption or overall production, the enzyme assays are used to monitor enzymatic activity. Provided with the required supplies and reagents for any preferred method, enzyme kinetics and inhibition studies can be successfully completed. While continuous assay sampling methods give activity readings throughout development, discontinuous techniques calculate after the reaction has been stopped. The standardized enzyme assays will provide accurate identification and measurement of the protein molecules present in samples.
Amplite® Rapid Colorimetric APC-Maleimide Quantitation Kit
Supplier: AAT Bioquest
Designed for consistent and dependable results. The Amplite® Rapid Colorimetric APC-Maleimide Quantitation Kit facilitates the quantification of maleimide groups on maleimide-activated APC proteins using a proprietary sensor, Maleimide 555™, which has an absorption peak at ~555 nm.
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Amplite® Fluorimetric Hypochlorite (Hypochlorous Acid) Assay Kit
Supplier: AAT Bioquest
Hypochlorite anion (ClO-) and its protonated form, hypochlorous acid (HClO) are critical reactive oxygen species (ROS) in biological systems.
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Amplite® Fluorimetric Glutathione GSH/GSSG Ratio Assay Kit *Green Fluorescence*
Supplier: AAT Bioquest
When cells are exposed to increased levels of oxidative stress, GSSG will accumulate and the ratio of GSH to GSSG will decrease.
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Amplite® Rapid Colorimetric Protein Aldehyde Content Quantitation Kit
Supplier: AAT Bioquest
Site-selective modification of proteins is of major interest in chemical biology.
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Amplite® Fluorimetric Mercuric Ion Quantitation Kit
Supplier: AAT Bioquest
Mercuric ion is an essential metal ion that plays an important role in a number of biological processes.
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Amplite® Fluorimetric Goat Anti-Mouse IgG-HRP Conjugate ELISA Assay Kit *Red Fluorescence*
Supplier: AAT Bioquest
Horseradish Peroxidase (HRP) is a small molecule (MW ~40 Kd) that is widely used in a variety of biological detections.
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Amplite® Choline Quantitation Kit
Supplier: AAT Bioquest
Choline and its metabolites play an important role in the structural integrity and signaling of cell membranes and cholinergic neurotransmission (acetylcholine synthesis).
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ReadiUse™ NADP Regenerating Kit
Supplier: AAT Bioquest
Designed for consistent and dependable results. AAT Bioquest's ReadiUse™ NADP regenerating kit provides a flexible prepackaged NADP regenerating system to accommodate various experimental designs.
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Amplite® Colorimetric Aldehyde Quantitation Kit *Blue Color*
Supplier: AAT Bioquest
The formation, reactivity and toxicity of aldehydes originating from the peroxidation of lipids of cellular membranes have received great attention in recent years.
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Amplite® Blue Peroxidase Substrate
Supplier: AAT Bioquest
A variety of substrates are available for detecting peroxidase activity in ELISA assays. colourimetric HRP substrates (e.g., TMB, OPD and ABTS) have been widely used for years. Each of these substrates varies greatly with respect to its performance characteristics such as detection sensitivity, working range and attainable signal-to-noise ratios. Amplite® Blue peroxidase substrate is a soluble fluorogenic substrate for detection of peroxidase activity. Amplite® Blue allows rapid HRP detection assays to be performed with greater sensitivity than the colourimetric HRP substrates (such as TMB and OPD). The fluorescent product of Amplite® Blue does not photobleach. Amplite® Blue exhibits a flat baseline in assays, which facilitates low-level detection sensitivity and allows for high signal-to-noise ratios.
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Amplite® Colorimetric Superoxide Dismutase (SOD) Assay Kit
Supplier: AAT Bioquest
Superoxide dismutases (SOD) are a class of enzymes that catalyse the dismutation of superoxide into oxygen and hydrogen peroxide.
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Amplite® Fluorimetric Melanin Assay Kit
Supplier: AAT Bioquest
Melanins have very diverse roles and functions in various organisms.
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Amplite® Luminometric Nitroreductase Assay Kit
Supplier: AAT Bioquest
Nitroreductases (NTR) are a family of evolutionarily related proteins involved in the reduction of nitrogen-containing compounds.
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Amplite® Colorimetric D-Lactate Assay Kit
Supplier: AAT Bioquest
Lactic acid is chiral and has two optical isomers: L-lactic acid and D-lactic acid.
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Amplite® Fluorimetric Ascorbic Acid Assay Kit
Supplier: AAT Bioquest
L-Ascorbic Acid (also called Vitamin C) is a critical metabolite for both plant and animals in cell division, growth and defense.
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Amplite® Colorimetric Beta-Hydroxybutyrate (Ketone Body) Assay Kit
Supplier: AAT Bioquest
Ketone bodies are produced by the liver and used peripherally as an energy source when blood glucose levels drop.
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Amplite® Colorimetric Fructose-6-Phosphate Activity Assay Kit
Supplier: AAT Bioquest
Designed for consistent and dependable results. The Amplite® colorimetric fructose-6-phosphate assay kit is a highly sensitive and simple method of quantifying F6P present in a variety of samples.
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Quantitative peroxide assay kit (aqueous), Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ Quantitative Peroxide Assay Kit (Aqueous) detects and measures hydrogen peroxide levels in biological samples using an iron and xylenol orange* (XO) reagent.
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Amplite® Colorimetric Hypochlorite (Hypochlorous Acid) Assay Kit
Supplier: AAT Bioquest
Hypochlorite anion (ClO-) and its protonated form, hypochlorous acid (HClO) are critical reactive oxygen species (ROS) in biological systems.
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Amplite® Colorimetric Sphingomyelinase Assay Kit *Blue Color*
Supplier: AAT Bioquest
Sphingomyelinase (SMase) is an enzyme that is responsible for cleaving sphingomyelin (SM) to phosphocholine and ceramide.
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Pierce™ Cypridina-Firefly Luciferase Dual Assay Kit
Supplier: Thermo Fisher Scientific
The Thermo Scientific Pierce Cypridina-Firefly Luciferase Dual Assay Kit provides the necessary reagents to simultaneously detect intracellular Cypridina and Red Firefly luciferase activity in mammalian whole cell lysates.
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Amplite® Luminometric Alkaline Phosphatase Assay Kit *Luminescence*
Supplier: AAT Bioquest
Alkaline phosphatase is a highly sensitive enzyme for ELISA, immuno-histochemical, Northern, Southern and Western blot applications.
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Amplite® Colorimetric Acetylcholinesterase Assay Kit
Supplier: AAT Bioquest
Acetylcholinesterase, also known as AChE, is an enzyme that degrades (through its hydrolytic activity) the neurotransmitter acetylcholine, producing choline and an acetate group.
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Phosphatase assay kit
Supplier: G-Biosciences
The Phosphatase Assay Kit is designed to measure the activity of phosphatases in biological samples and to screen for agonists and inhibitors of phosphatases. The Phosphatase Assay Kit uses para-nitrophenyl phosphate (pNPP), a chromogenic substrate for most phosphatases, including alkaline phosphatases, acid phosphatases, protein tyrosine phosphatases and serine/threonine phosphatases.
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Amplite® Fluorimetric Acetylcholinesterase Assay Kit *Red Fluorescence*
Supplier: AAT Bioquest
Acetylcholinesterase, also known as AChE, is an enzyme that degrades (through its hydrolytic activity) the neurotransmitter acetylcholine, producing choline and an acetate group.
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Amplite® Colorimetric Bradford Protein Quantitation Assay Kit
Supplier: AAT Bioquest
The traditional Bradford protein assay is widely used for quantifying protein concentrations.
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Amplite® Fluorimetric NAD Assay Kit *Blue Fluorescence*
Supplier: AAT Bioquest
Nicotinamide adenine dinucleotide (NADH) and its oxidized form (NAD) are essential cofactors for many enzyme reactions found in living cells.
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Amplite® Fluorimetric L-Alanine Assay Kit
Supplier: AAT Bioquest
L-alanine (L-Ala) plays a crucial role as a building block of important proteins.
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Amplite® Fluorimetric Maleimide Quantitation Kit *Green Fluorescence*
Supplier: AAT Bioquest
A variety of crosslinking reagents with a maleimide group are widely used for crosslinking proteins to proteins or proteins to other biomolecules.
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Amplite® Fluorimetric Beta-Galactosidase Assay Kit *Green Fluorescence*
Supplier: AAT Bioquest
E. coli beta-galactosidase is a 464 Kd tetramer.