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Either measuring substrate consumption or overall production, the enzyme assays are used to monitor enzymatic activity. Provided with the required supplies and reagents for any preferred method, enzyme kinetics and inhibition studies can be successfully completed. While continuous assay sampling methods give activity readings throughout development, discontinuous techniques calculate after the reaction has been stopped. The standardized enzyme assays will provide accurate identification and measurement of the protein molecules present in samples.

Amplite® Ethanol Quantitation Kit

Amplite® Ethanol Quantitation Kit

Supplier: AAT Bioquest

The ability to rapidly perform quantitative measurements of ethanol is highly desirable in life science research, clinical evaluations, and food and drug industries.

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Peroxide assay kit, PEROXsay™

Peroxide assay kit, PEROXsay™

Supplier: G-Biosciences

PEROXsay™ is a colorimetric quantitative peroxide assay that measures the oxidation of ferrous (Fe²⁺+) ions to ferric (Fe³⁺) ions. Peroxides react with a sugar alcohol converting it to a peroxyl radical that subsequently starts the oxidation of ferrous ions to ferric ions. The acidic pH of the assay allows the ferric (Fe³⁺) ion to complex with the colorimetric reagent, resulting in a change in absorbance that is proportional to the peroxide concentration.

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Amplite® Fluorimetric Protein Quantitation Kit *Orange Fluorescence*

Amplite® Fluorimetric Protein Quantitation Kit *Orange Fluorescence*

Supplier: AAT Bioquest

Protein quantification is an essential task in protein purification, electrophoresis, cell biology, molecular biology and other research applications.

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Amplite® Fluorimetric Aldehyde Quantitation Kit

Amplite® Fluorimetric Aldehyde Quantitation Kit

Supplier: AAT Bioquest

The formation, reactivity and toxicity of aldehydes originating from the peroxidation of lipids of cellular membranes have received great attention in recent years.

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Amplite® Fluorimetric Calcium Quantitation Kit  *Red Fluorescence*

Amplite® Fluorimetric Calcium Quantitation Kit *Red Fluorescence*

Supplier: AAT Bioquest

Calcium is essential for all living organisms, particularly in cell physiology, where movement of the calcium ion Ca²⁺ into and out of the cytoplasm functions as a signal for many cellular processes.

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Serine hydrolase probes, ActivX™ FP

Serine hydrolase probes, ActivX™ FP

Supplier: Thermo Fisher Scientific

The ActivX® FP serine hydrolase probes enable selective labeling and enrichment of active serine hydrolases. Fluorophosphonate (FP) probes specifically label serines of enzymatically active serine hydrolases. FP probes are also effective for screening small molecule inhibitors against enzymes derived from cell lysates, subcellular fractions, tissues and recombinant proteins. Depending on the active-site probe tag group and the enzyme class to be profiled, active site-labeled enzymes are detected and quantified by Western blot, fluorescent gel imaging or mass spectrometry.

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Amplite® Rapid Colorimetric PE-Maleimide Quantitation Kit

Supplier: AAT Bioquest

Designed for consistent and dependable results. The Amplite® rapid colorimetric PE-maleimide quantitation kit facilitates the quantification of maleimide groups on maleimide-activated PE proteins using a proprietary sensor, Maleimide 680™, which has an absorption peak at ~680 nm.

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Amplite® Fluorimetric Acidic Sphingomyelinase Assay Kit *Red Fluorescence*

Amplite® Fluorimetric Acidic Sphingomyelinase Assay Kit *Red Fluorescence*

Supplier: AAT Bioquest

Sphingomyelinase (SMase) is an enzyme that is responsible for cleaving sphingomyelin (SM) to phosphocholine and ceramide.

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Amplite® Colorimetric D-Lactate Dehydrogenase (LDH) Assay Kit

Amplite® Colorimetric D-Lactate Dehydrogenase (LDH) Assay Kit

Supplier: AAT Bioquest

Lactate dehydrogenase (LDH) is an oxidoreductase enzyme that catalyses the interconversion of pyruvate and lactate.

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Amplite® Colorimetric Beta-Galactosidase Assay Kit

Amplite® Colorimetric Beta-Galactosidase Assay Kit

Supplier: AAT Bioquest

E. coli beta-galactosidase is a 464 kD tetramer.

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Amplite® Colorimetric Beta-Lactamase Activity Assay Kit

Amplite® Colorimetric Beta-Lactamase Activity Assay Kit

Supplier: AAT Bioquest

β-lactamases are a large family of enzymes capable of hydrolysing β-lactams.

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Amplite® Fluorimetric NADP Assay Kit *Blue Fluorescence*

Amplite® Fluorimetric NADP Assay Kit *Blue Fluorescence*

Supplier: AAT Bioquest

Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors for many enzyme reactions found in living cells.

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Amplite® Fluorimetric D-Lactate Dehydrogenase (LDH) Assay Kit

Amplite® Fluorimetric D-Lactate Dehydrogenase (LDH) Assay Kit

Supplier: AAT Bioquest

Lactate dehydrogenase (LDH) is an oxidoreductase enzyme that catalyses the interconversion of pyruvate and lactate.

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Amplite® Colorimetric Caspase 3/7 Assay Kit *Yellow Color*

Amplite® Colorimetric Caspase 3/7 Assay Kit *Yellow Color*

Supplier: AAT Bioquest

Caspases play important roles in apoptosis and cell signaling.

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Amplite® Fluorimetric Glutamic Acid Assay Kit *Red Fluorescence*

Amplite® Fluorimetric Glutamic Acid Assay Kit *Red Fluorescence*

Supplier: AAT Bioquest

Glutamic acid is one of the 20 proteinogenic amino acids.

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Amplite® Luminometric Peroxidase (HRP) Assay Kit

Amplite® Luminometric Peroxidase (HRP) Assay Kit

Supplier: AAT Bioquest

Peroxidase is a small molecule (MW ~40 KD) that can usually be conjugated to an antibody in a 4:1 ratio.

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Amplite® Colorimetric L-Lactate Assay Kit

Amplite® Colorimetric L-Lactate Assay Kit

Supplier: AAT Bioquest

Lactic acid is chiral and has two optical isomers: L-lactic acid and D-lactic acid.

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Amplite® Fluorimetric Renin Assay Kit *Red Fluorescence*

Amplite® Fluorimetric Renin Assay Kit *Red Fluorescence*

Supplier: AAT Bioquest

Renin is an enzyme that participates in the body's renin-angiotensin system (RAS) that mediates extracellular volume and arterial vasoconstriction.

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Amplite® Fluorimetric Glutathione Peroxidase Assay Kit *Blue Fluorescence*

Amplite® Fluorimetric Glutathione Peroxidase Assay Kit *Blue Fluorescence*

Supplier: AAT Bioquest

Glutathione peroxidase is an enzyme family with peroxidase activity to protect the organism from oxidative damage.

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Amplite® Fluorimetric Peroxidase (HRP) Assay Kit *Red Fluorescence*

Amplite® Fluorimetric Peroxidase (HRP) Assay Kit *Red Fluorescence*

Supplier: AAT Bioquest

Peroxidase is a small molecule (MW ~40 KD) that can usually be conjugated to an antibody in a 4:1 ratio.

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Amplite® Colorimetric Antioxidant Activity Assay Kit

Amplite® Colorimetric Antioxidant Activity Assay Kit

Supplier: AAT Bioquest

Reactive oxygen species (ROS) are essential in cell signaling and maintaining physiological cell metabolism.

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Amplite® Colorimetric Lipase Activity Assay Kit

Supplier: AAT Bioquest

Designed for consistent and dependable results. The Amplite® colorimetric lipase activity assay kit offers a straightforward and efficient protocol for quantifying lipase activity.

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Amplite® Fluorimetric Neuraminidase Assay Kit *Blue Fluorescence*

Amplite® Fluorimetric Neuraminidase Assay Kit *Blue Fluorescence*

Supplier: AAT Bioquest

Neuraminidases, also called sialidases, are glycoside hydrolase enzymes that catalyze the hydrolysis of terminal sialic acid residues and neuraminic acid.

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Amplite® Fluorimetric Lysyl Oxidase Assay Kit *Red Fluorescence*

Amplite® Fluorimetric Lysyl Oxidase Assay Kit *Red Fluorescence*

Supplier: AAT Bioquest

Lysyl oxidase (LOX) is an extracellular enzyme that catalyses formation of aldehydes from lysine residues in collagen and elastin precursors.

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Amplite® Colorimetric Enterokinase Activity Assay Kit

Amplite® Colorimetric Enterokinase Activity Assay Kit

Supplier: AAT Bioquest

Enterokinase (also called enteropeptidase) is a serine protease produced by cells in the duodenal wall and is a key enzyme in human and animal digestion system.

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Amplite® Universal Fluorimetric MMP Activity Assay Kit *Red Fluorescence*

Amplite® Universal Fluorimetric MMP Activity Assay Kit *Red Fluorescence*

Supplier: AAT Bioquest

The matrix metalloproteinases (MMPs) constitute a family of zinc-dependent endopeptidases that function within the extracellular matrix.

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Amplite® Colorimetric Pyruvate Assay Kit

Amplite® Colorimetric Pyruvate Assay Kit

Supplier: AAT Bioquest

Pyruvate is an important chemical compound in intracellular metabolic pathways.

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Amplite® Total Sulfide Quantification Kit

Amplite® Total Sulfide Quantification Kit

Supplier: AAT Bioquest

Hydrogen sulfide is not usually a health risk at concentrations present in household water.

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Amplite® Fluorimetric Glucose Quantitation Kit

Amplite® Fluorimetric Glucose Quantitation Kit

Supplier: AAT Bioquest

Glucose, a monosaccharide, is the most important carbohydrate in biology.

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Amplite® Colorimetric Maleimide Quantitation Kit

Amplite® Colorimetric Maleimide Quantitation Kit

Supplier: AAT Bioquest

Maleimides can be directly assayed spectrophotometrically at 302 nm.

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