You searched for: Enzyme Assays
Either measuring substrate consumption or overall production, the enzyme assays are used to monitor enzymatic activity. Provided with the required supplies and reagents for any preferred method, enzyme kinetics and inhibition studies can be successfully completed. While continuous assay sampling methods give activity readings throughout development, discontinuous techniques calculate after the reaction has been stopped. The standardized enzyme assays will provide accurate identification and measurement of the protein molecules present in samples.
Matrix Metalloproteinase-14 (MMP-14) fluorometric drug discovery kit
Supplier: Enzo Life Sciences
A QuantiZyme™ Assay System. The MMP-14 Fluorometric (also known as fluorimetric) Drug Discovery Kit is a complete assay sytem designed to screen for inhibitors of matrix metalloproteinase-14 (MMP-14, membrane-type matrix metalloproteinase 1, MT1-MMP) using a quenched fluorogenic peptide: OmniMMP™ fluorogenic substrate Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH. Mca fluorescence is quenched by the Dpa group until cleavage by MMPs at the Gly-Leu bond separates the two moieties.
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Neutrophil Elastase Activity Assay Kit
Supplier: Cayman Chemical
A convenient all-in-one tool to measure neutrophil elastase activity.
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Fluorometric polymerase activity assay kit, EvaEZ™
Supplier: Biotium
EvaEZ™ Fluorometric Polymerase Activity Assay Kit provides an easy and accurate way to determine the activity of a nucleic acid polymerase without using radioisotopes.
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DPPIV Drug discovery kit
Supplier: Enzo Life Sciences
The DPPIV Drug Discovery Kit is a complete assay system designed to screen DPPIV inhibitors, a potential therapeutic target, providing enough material to perform at least 96 assays.
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Glutaminase (GLS1) Inhibitor Screening Kit (Fluorometric)
Supplier: Abcam
Sensitive, reproducible assay for initial screening of potential glutaminase inhibitors.
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Fluorometric drug discovery assay kit, SIRT5, Fluor-de-Lys®
Supplier: Enzo Life Sciences
The SIRT5 Fluorometric Drug Discovery Kit is a sensitive fluorometric assay is designed to measure the lysyl desuccinylase activity of the recombinant human SIRT5.
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Granzyme B assay kit for drug discovery
Supplier: Enzo Life Sciences
The Granzyme B Assay Kit for Drug Discovery is a complete assay system designed to measure the protease activity of granzyme B. It contains a colorimetric substrate (IEPD-pNA) to measure this activity; cleavage of the p-nitroanilide (pNA) group from the colorimetric substrate increases absorption at 405 nm.
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Matrix Metalloproteinase-2 (MMP-2) fluorometric drug discovery kit
Supplier: Enzo Life Sciences
A Quantizyme™ Assay System. The MMP-2 Fluorometric (also known as fluorimetric) Drug Discovery Kit is a complete assay system designed to screen inhibitors of matrix metalloproteinase-2 (MMP-2, gelatinase A) using a quenched fluorogenic peptide: OmniMMP™ fluorogenic substrate Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH. Mca fluorescence is quenched by the Dpa group until cleavage by MMPs at the Gly-Leu bond separates the two moieties. The assays are performed in a convenient 96-well microplate format. The kit is useful to screen inhibitors of MMP-2, a potential therapeutic target.
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SAM Methyltransferase assay, SAMfluoro™
Supplier: G-Biosciences
The SAMfluoro™ assay is a fluorescent, continuous enzyme coupled assay that can continuously monitor SAM-dependent methyltransferases without the use of radioactive labels or endpoint measurements.
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Amplite® Colorimetric Phosphofructokinase (PFK) Activity Assay Kit
Supplier: AAT Bioquest
Designed for consistent and dependable results. The Amplite® Colorimetric Phosphofructokinase (PFK) Activity Assay Kit provides a simple and direct procedure for precise measurement of PFK enzyme activity in various biological samples.
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Amplite® Universal Fluorimetric Kinase Assay Kit *Red Fluorescence*
Supplier: AAT Bioquest
The Amplite® Universal Kinase Assay Kit is based on the monitoring of ADP formation, which is directly proportional to enzyme phoshphotransferase activity and is measured fluorimetricaly. This kit provides a fast, simple, and homogeneous assay for measure kinases activities. The characteristics of its high sensitivity (<0.2 uM ADP), broad ATP tolerance (1-300 uM), non-antibody based, non-radioactive and no-wash method to detect the amount of ADP produced as a result of enzyme activity make it an ideal kit for determining kinase Michaelis-Menten kinetics and for screening and identifying kinase inhibitors. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation with no separation steps required.
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Amplite® Fluorimetric Acidic Sphingomyelinase Assay Kit *Red Fluorescence*
Supplier: AAT Bioquest
Sphingomyelinase (SMase) is an enzyme that is responsible for cleaving sphingomyelin (SM) to phosphocholine and ceramide.
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Amplite® Colorimetric D-Lactate Dehydrogenase (LDH) Assay Kit
Supplier: AAT Bioquest
Lactate dehydrogenase (LDH) is an oxidoreductase enzyme that catalyses the interconversion of pyruvate and lactate.
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Amplite® Colorimetric Beta-Galactosidase Assay Kit
Supplier: AAT Bioquest
E. coli beta-galactosidase is a 464 kD tetramer.
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Amplite® Fluorimetric NADP Assay Kit *Blue Fluorescence*
Supplier: AAT Bioquest
Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors for many enzyme reactions found in living cells.
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Amplite® Colorimetric Caspase 3/7 Assay Kit *Yellow Color*
Supplier: AAT Bioquest
Caspases play important roles in apoptosis and cell signaling.
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Amplite® Fluorimetric Glutamic Acid Assay Kit *Red Fluorescence*
Supplier: AAT Bioquest
Glutamic acid is one of the 20 proteinogenic amino acids.
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Amplite® Luminometric Peroxidase (HRP) Assay Kit
Supplier: AAT Bioquest
Peroxidase is a small molecule (MW ~40 KD) that can usually be conjugated to an antibody in a 4:1 ratio.
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Amplite® Colorimetric L-Lactate Assay Kit
Supplier: AAT Bioquest
Lactic acid is chiral and has two optical isomers: L-lactic acid and D-lactic acid.
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Amplite® Fluorimetric Peroxidase (HRP) Assay Kit *Red Fluorescence*
Supplier: AAT Bioquest
Peroxidase is a small molecule (MW ~40 KD) that can usually be conjugated to an antibody in a 4:1 ratio.
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Amplite® Colorimetric Antioxidant Activity Assay Kit
Supplier: AAT Bioquest
Reactive oxygen species (ROS) are essential in cell signaling and maintaining physiological cell metabolism.
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Amplite® Colorimetric Lipase Activity Assay Kit
Supplier: AAT Bioquest
Designed for consistent and dependable results. The Amplite® colorimetric lipase activity assay kit offers a straightforward and efficient protocol for quantifying lipase activity.
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Amplite® Universal Fluorimetric MMP Activity Assay Kit *Red Fluorescence*
Supplier: AAT Bioquest
The matrix metalloproteinases (MMPs) constitute a family of zinc-dependent endopeptidases that function within the extracellular matrix.
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Amplite® Total Sulfide Quantification Kit
Supplier: AAT Bioquest
Hydrogen sulfide is not usually a health risk at concentrations present in household water.
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Amplite® Fluorimetric Glucose Quantitation Kit
Supplier: AAT Bioquest
Glucose, a monosaccharide, is the most important carbohydrate in biology.
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Amplite® Colorimetric Tyrosinase Assay Kit
Supplier: AAT Bioquest
Tyrosinase is of great interest to drug discovery, life science research, food industry and cosmetics industry since it plays an important role in the biosynthetic pathway of melanin.
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Amplite® Fluorimetric NADP/NADPH Ratio Assay Kit *Red Fluorescence*
Supplier: AAT Bioquest
Nicotinamide adenine dinucleotide (NAD⁺) and nicotinamide adenine dinucleotide phosphate (NADP⁺) are two important cofactors found in cells.
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Amplite® IR
Supplier: AAT Bioquest
Our Amplite® IR is a fluorogenic peroxidase substrate that generates near infrared fluorescence upon reaction with peroxidase and H2O2.
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Amplite® Fluorimetric Malondialdehyde (MDA) Quantitation Kit
Supplier: AAT Bioquest
Malondialdehyde (MDA) is one of the natural byproducts during lipid peroxidation.
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Amplite® Fluorimetric Glucose Oxidase Assay Kit *Red Fluorescence*
Supplier: AAT Bioquest
The glucose oxidase is a dimeric protein that catalyses the oxidation of beta-D-glucose into hydrogen peroxide and D-glucono-1,5-lactone, which is hydrolyzed to gluconic acid.
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Supplier: GRANT INSTRUMENTS
Supplier: BOCHEM
Supplier: VWR Collection
Supplier: OMEGA BIO-TEK
Supplier: DWK Life Sciences
Supplier: Thermo Fisher Scientific
Supplier: VWR Collection
Supplier: Oxford Nanopore Technologies
Supplier: Industrial Physics
Supplier: AAT Bioquest
Supplier: Integra
Supplier: Palbam
Supplier: VWR Collection
Supplier: Integra
Supplier: VWR Collection
Supplier: FACELLITATE
Supplier: NANOENTEK
Supplier: Greiner Bio-One