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Buněčná stanovení

Buněčná stanovení

Buněčné testy byly vždy silným nástrojem ve výzkumné laboratoři. Jsou mimořádně všestranné a lze je navrhnout tak, aby měřily prakticky jakoukoli buněčnou nebo biochemickou funkci. Citlivá detekce fluorescenčních sond pomáhá při hodnocení buněčné signalizace a drah buněčné smrti, což je užitečné pro rychlou kvantifikaci životaschopnosti buněk. Buněčné testy lze použít k měření účinků různých ošetření na životaschopnost buněk a k hodnocení toxických účinků zprostředkovaných různými mechanismy.

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RedDot™1 Far-Red Nuclear Stain, 200X in Water, Biotium

RedDot™1 Far-Red Nuclear Stain, 200X in Water, Biotium

Supplier: Biotium

RedDot™1 is a far-red cell membrane-permeable nuclear dye similar to Draq5™. The dye is ideal for specifically staining the nuclei of live cells.

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FLUOR DE LYS®-Green HDAC2 fluorometric drug discovery assay kit

Supplier: Enzo Life Sciences

The HDAC2 Fluorescent Activity Assay/Drug Discovery Kit is a complete assay system designed to measure the lysyl deacetylase activity of the recombinant human HDAC2 included in the kit. The kit is ideal for chemical library screening for candidate inhibitors or kinetic assay of the enzyme under varying conditions.

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Fluorescent calcium indicator, GFP-CERTIFIED® FLUOFORTE®

Supplier: Enzo Life Sciences

GFP-CERTIFIED® FLUOFORTE® is a fluorescent calcium indicator relative to other red fluorescent dyes.

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FLUOR DE LYS® HDAC6 fluorometric drug discovery kit

Supplier: Enzo Life Sciences

A Fluor-de-Lys® fluorescent assay system. The HDAC6 Fluorescent Activity Assay/Drug Discovery Kit is a complete assay system designed to measure the lysyl deacetylase activity of the recombinant human HDAC6 included in the kit. The kit is ideal for chemical library screening for candidate inhibitors or activators or kinetic assay of the enzyme under varying conditions. The Fluor de Lys® HDAC6 assay is based on the Fluor de Lys® Substrate and Fluor de Lys® Developer combination. The assay procedure has two steps. First, the Fluor de Lys® Substrate, which comprises an acetylated lysine side chain, is incubated with HDAC6. Deacetylation of the substrate sensitizes the substrate so that, in the second step, treatment with the Fluor de Lys®Developer produces a fluorophore.

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Uric acid assay kit

Uric acid assay kit

Supplier: Cayman Chemical

Uric Acid Assay Kit is used to measure uric acid in serum, plasma and urine.

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Prostaglandin Screening Library I

Prostaglandin Screening Library I

Supplier: Cayman Chemical

For screening of PGF analogs.

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NUCLEAR-ID® green cell cycle kit for flow cytometry

Supplier: Enzo Life Sciences

For cell cycle results independent of incubation time, temperature, dye and cell concentrations.

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Green detection kit, MITO-ID®

Supplier: Enzo Life Sciences

MITO-ID® Green Detection Kit is a potent mitochondria-selective dye suitable for live, permeabilised and fixed cell staining.

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Solutions colorantes, Ghost Dye™

Solutions colorantes, Ghost Dye™

Supplier: Tonbo Biosciences

Ghost Dye solutions allow for clear live vs. dead cell discrimination for more precise flow cytometry analysis, and are especially useful for intracellular staining protocols.

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ViaFluor® 647 live cell microtubule stain 1000X in DMSO

Supplier: Biotium

Stain microtubules in live cells with far-red fluorescence.

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ViaFluor® 405 live cell microtubule stain 1000X in DMSO

Supplier: Biotium

Stain microtubules in live cells with blue fluorescence.

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Comet SCGE assay kit

Supplier: Enzo Life Sciences

COMET SCGE Assay measures DNA damage by fluorescently detecting the integrity of DNA liberated from cells embedded in low melting point agarose. Upon electrophoresis, fragmented DNA produces a characteristic "comet" shaped tail as small DNA fragments migrate in the gel more rapidly than in-tact genomic DNA.

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Apoptosis assay kit (Dual), NucView™ 488

Apoptosis assay kit (Dual), NucView™ 488

Supplier: Biotium

NucView® 488 and CF™dye (594, 640R and Texas RED) conjugated annexin V provides two apoptosis events, caspase-3 activity and phosphatidylserine (PS) translocation.

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Red detection kit (GFP-CERTIFIED®), MITO-ID®

Supplier: Enzo Life Sciences

MITO-ID® Red Detection Kit (GFP-CERTIFIED®) is a photostable, non-toxic and selective mitochondrial dye that stains live, detergent permeabilised and aldehyde fixed cells. This kit is specifically designed for use with GFP expressing cell lines, as well as cells expressing blue, cyan or yellow fluorescent proteins (BFP's, CFP's, YFP's).

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Reagents for Fluor-de-Lys®-Green HDAC fluorometric activity assay kit

Supplier: Enzo Life Sciences

Cellular assay, Nicotinamide adenine dinucleotide (oxidised form) in 50 mM Tris/HCl and sirutin substrate

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Steady-Luc™ Firefly HTS assay kit

Steady-Luc™ Firefly HTS assay kit

Supplier: Biotium

Firefly luciferase is widely used as a transcriptional reporter gene for research and drug screening. The Steady-Luc™ HTS kit can be used with luminescence plate readers without sample injection systems or for high-throughput screening.

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