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552 results for Cellular Assays

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Cellular Assays

Cellular assays have always been a powerful tool in the research lab. They are incredibly versatile, and can be designed to measure virtually any cellular or biochemical function. Sensitive detection of fluorescent probes aid in evaluating cell signaling and death pathways, which is useful for the rapid quantification of cell viability. Cellular assays can be used to measure the effects of different treatments on cell viability and to evaluate toxic effects mediated through a variety of mechanisms.

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Nitric Oxide (NO2-/NO3-) detection kit

Supplier: ENZO LIFE SCIENCES

For the quantitative determination of nitrite and nitrate levels in culture supernatants, plasma, serum, saliva, and urine from any species.

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GFP-CERTIFIED® FLUOFORTE® Calcium assay kit

Supplier: ENZO LIFE SCIENCES

The GFP-Certified™ FluoForte™ Calcium Assay kit provides a homogeneous fluorescence-based assay for detecting intracellular calcium mobilization across a broad spectrum of biological targets.

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FLUOR DE LYS®-Green HDAC2 fluorometric drug discovery assay kit

Supplier: ENZO LIFE SCIENCES

The HDAC2 Fluorescent Activity Assay/Drug Discovery Kit is a complete assay system designed to measure the lysyl deacetylase activity of the recombinant human HDAC2 included in the kit. The kit is ideal for chemical library screening for candidate inhibitors or kinetic assay of the enzyme under varying conditions.

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Steady membrane staining kits, CellBrite™

Steady membrane staining kits, CellBrite™

Supplier: Biotium

Cell surface staining kits with fluorescent membrane dye plus enhancer for imaging live cell surface for several hours to days.

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CV-Caspase 3 & 7 Detection kit

Supplier: ENZO LIFE SCIENCES

Based on the dye cresyl violet and the caspase 3/7 DEVD peptide recognition sequence, this kit allows the detection of caspases 3 and 7 in living cells by fluorescence microscopy.

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TOTAL-NUCLEAR-ID® green/red nucleolar/nuclear detection kit

Supplier: ENZO LIFE SCIENCES

The kit contains two proprietary dyes suitable for visualizing nucleoli and nuclei simultaneously in living cells.

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Apoptosis inducer set

Apoptosis inducer set

Supplier: G-Biosciences

Apoptosis inducers are used for induction of apoptosis in cultured cells. The selection of apoptosis inducers include actinomycin D, camptothecin, cycloheximide, dexamethasone, doxorubicin and etoposide.

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Fluor-de-Lys® SIRT2 fluorometric drug discovery assay kit

Supplier: ENZO LIFE SCIENCES

A Fluor-de-Lys® fluorescent assay system. The SIRT2 Fluorescent Activity Assay/Drug Discovery Kit is a complete assay system designed to measure the lysyl deacetylase activity of the recombinant human SIRT2 included in the kit. The kit is ideal for chemical library screening for candidate inhibitors or activators or kinetic assay of the enzyme under varying conditions.

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ViaFluor® 405 live cell microtubule stain 1000X in DMSO

Supplier: Biotium

Stain microtubules in live cells with blue fluorescence.

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Annexin V CF® dye conjugates, azide-free, lyophilised

Supplier: Biotium

Preservative-free Annexin V conjugates are compatible with detection of apoptotic cells in live cell imaging experiments, fluorescence microscopy, or flow cytometry.

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Caspase-3 assay kits, NucView™ 488

Caspase-3 assay kits, NucView™ 488

Supplier: Biotium

NucView® 488 caspase-3 substrate is a novel cell membrane-permeable fluorogenic caspase substrate designed for detecting caspase-3 activity within live cells in real time.

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NUCLEOLAR-ID® Green detection kit

Supplier: ENZO LIFE SCIENCES

Specifically designed for visualizing microscopically nucleoli in living cells.

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CREB (Phospho-Ser133) transcription factor assay kit

Supplier: Cayman Chemical

A 96-well assay for measurement of CREB.

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CV-Cathepsin B detection kit

Supplier: ENZO LIFE SCIENCES

Based on the dye cresyl violet and the cathepsin B ArgArg peptide recognition sequence, this kit allows the detection of cathepsin B activity in living cells by fluorescence microscopy.

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Cell proliferation assay kit, WST-1

Supplier: Cayman Chemical

WST-1 Proliferation Assay Kit provides an easy to use tool for studying the induction and inhibition of cell proliferation in any in vitro model. The assay is based on the reduction of tetrazolium salt WST-1 to soluble formazan by electron transport across the plasma membrane of dividing cells. This kit will also allow investigators to screen drug candidates involved in regulation of cell cycle.

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Red hydrogen peroxide assay kit

Supplier: ENZO LIFE SCIENCES

Red Hydrogen Peroxide Assay Kit provides a simple absorbance-based or fluorometric assay to detect and quantify hydrogen peroxide or peroxidase activity in biochemical assays, cell extracts and in live cells. It can also be used to detect a variety of oxidase activities through enzyme-coupled reactions. The kit is an optimized ‘mix and read’ assay that is suitable for both 96- and 348-well formats and is compatible with HTS liquid handling.

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Prostaglandin Screening Library II

Prostaglandin Screening Library II

Supplier: Cayman Chemical

For D and E series prostaglandin analog screening.

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Bacterial Ssaining, viability/cytotoxicity assay kit for bacteria live and dead cells

Supplier: Biotium

The Bacteria Viability/Cytotoxicity Assay Kit provides two-colour fluorescence staining of both live bacteria (green) and dead bacteria (red) using two probes, DMAO and EthD-III.

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Bacterial viability and gram stain kit

Supplier: Biotium

The Bacterial Viability and Gram Stain Kit contains three components: CF™488A-WGA, DAPI, and EthD-III solutions for distinguishing between gram-negative and gram-positive, as well as live versus dead bacteria.

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CF® Dye Dextran 150000 MW, anionic and fixable

CF® Dye Dextran 150000 MW, anionic and fixable

Supplier: Biotium

CF® dye labeled dextrans could be used as a fluorescent fluid-phase markers to study cell permeability, endocytosis, or mechanisms of biomolecular delivery. The dextran is 150,000 MW, and contains a formaldehyde-fixable free-amine group.

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RedDot™2 Far-Red Nuclear Stain, 200X in DMSO, Biotium

RedDot™2 Far-Red Nuclear Stain, 200X in DMSO, Biotium

Supplier: Biotium

RedDot™2 is a far-red cell membrane-impermeant nuclear dye similar to Draq7™. Ideal for staining the nuclei of dead or fixed and permeabilized cells with minimal cytoplasmic RNA staining.

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JC-1 Mitochondrial Membrane Potential Detection Kit, Biotium

JC-1 Mitochondrial Membrane Potential Detection Kit, Biotium

Supplier: Biotium

JC-1 dye is used for fluorescence-based measurement of mitochondrial membrane potential changes in cells, a key measure of cell viability.

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Flow cytometry fixation/permeabilisation kit

Flow cytometry fixation/permeabilisation kit

Supplier: Biotium

Flow Cytometry Fixation/Permeabilisation Kit contains optimally formulated buffers for fixation and permeabilisation of suspension cells for immunofluorescence staining of intracellular antigens for analysis by flow cytometry. The bufffers are also available separately.

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ApoSENSOR™ Cell viability assay kit

Supplier: ENZO LIFE SCIENCES

The assay utilizes bioluminescent detection of the ATP level for a rapid screening of apoptosis and cell proliferation simultaneously in mammalian cells. The assay utilizes the enzyme luciferase to catalyze the formation of light from ATP and luciferin. The light can be measured using a luminometer or Beta Counter. The assay can be fully automatic for high throughput (10 seconds/sample) and is highly sensitive (detects 10-100 mammalian cells/well).

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Green detection kit, MITO-ID®

Supplier: ENZO LIFE SCIENCES

MITO-ID® Green Detection Kit is a potent mitochondria-selective dye suitable for live, permeabilised and fixed cell staining.

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Lyso-ID® red detection kit (GFP-CERTIFIED®)

Lyso-ID® red detection kit (GFP-CERTIFIED®)

Supplier: ENZO LIFE SCIENCES

For use with GFP-expressing cell lines, as well as cells expressing blue, cyan or yellow fluorescent proteins.

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APO-BRDU™ kit, TUNEL Assay

APO-BRDU™ kit, TUNEL Assay

Supplier: Tonbo Biosciences

The APO-DIRECT™ reagent kit provides a two color TUNEL (Terminal deoxynucleotide transferase dUTP Nick End Labelling) assay for labeling DNA breaks and total cellular DNA to detect apoptotic cells by flow cytometry. The kit contains the instructions and reagents required for measuring apoptosis in cells including: positive and negative control cells for assessing reagent performance; washing, reaction and rinsing buffers for processing individual steps in the assay; terminal deoxynucleotidyl tranferase enzyme (TdT), fluorescein isothiocyanate (FITC) deoxyuridine triphosphate (FITC-dUTP) for labeling DNA breaks and PI/RNase A solution for counter staining the total DNA.

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Apoptosis/Necrosis Detection Kits, for microscopy and flow cytometry, GFP-Certified™

Apoptosis/Necrosis Detection Kits, for microscopy and flow cytometry, GFP-Certified™

Supplier: ENZO LIFE SCIENCES

GFP-Certified™ Apoptosis/Necrosis Detection Kit is a multiplex assay that distinguishes between healthy, early apoptotic, late apoptotic and necrotic cells, compatible with GFP and other green fluorescent probes.

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ALDetect™ (MDA-Specific) Lipid Peroxidation Assay kit

Supplier: ENZO LIFE SCIENCES

Lipid peroxidation is a well-established mechanism of cellular injury in both plants and animals, and is used as an indicator of oxidative stress in cells and tissues. Lipid peroxides, derived from polyunsaturated fatty acids, are unstable and decompose to form a complex series of compounds. These include reactive aldehydes, of which the most abundant is malondialdehyde (MDA). Therefore, measurement of malondialdehyde is widely used as an indicator of lipid peroxidation. Increased levels of lipid peroxidation products have been associated with a variety of chronic diseases in both humans and model systems.

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Autophagy detection tests, CYTO-ID®

Supplier: ENZO LIFE SCIENCES

CYTO-ID can monitor autophagy in live cells and autophagic flux in lysosomally inhibited live cells using a novel dye that selectively labels accumulated autophagic vacuoles.

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