Cellular assays have always been a powerful tool in the research lab. They are incredibly versatile, and can be designed to measure virtually any cellular or biochemical function. Sensitive detection of fluorescent probes aid in evaluating cell signaling and death pathways, which is useful for the rapid quantification of cell viability. Cellular assays can be used to measure the effects of different treatments on cell viability and to evaluate toxic effects mediated through a variety of mechanisms.
Cellular senescence activity assay
Supplier: ENZO LIFE SCIENCES
Highly reproducible SA-β-gal activity kit
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Calcineurin cellular activity assay kit
Supplier: ENZO LIFE SCIENCES
A complete colorimetric assay kit for measuring cellular calcineurin (PP2B) phosphatase activity. It employs a convenient 96-well plate format with all reagents necessary for measuring calcineurin (PP2B) phosphatase activity in tissue/cellular extracts PLUS human recombinant calcineurin is included as a positive control.
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Cellular senescence live cell analysis assay
Supplier: ENZO LIFE SCIENCES
Simple assay to quantify senescence-associated β-galactosidase activity, in live cells, using flow cytometry or fluorescent microscopy.
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FLUOR DE LYS® HDAC fluorometric cellular activity assay kit
Supplier: ENZO LIFE SCIENCES
The HDAC fluorometric cellular activity assay allows the determination of deacetylase activity within an undisturbed cellular environment and provides accurate activity information that is reflective of endogenous regulation. It also allows the study of the effects of upstream regulators on deacetylase activity and the detection of inhibitors or activators that act indirectly to affect deacetylase activity.
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Glucose Uptake Cell-Based Assay Kit
Supplier: Cayman Chemical
A cell-based assay for studying modulators of cellular glucose uptake.
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Caspase-3 cell assay kit
Supplier: ENZO LIFE SCIENCES
This kit is a complete assay system for measuring caspase-3 and caspase-3-like activity (DEVDase) in cellular extracts.
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Cell-based assay calcein AM
Supplier: Cayman Chemical
Cellular assay, Cell-based assay calcein AM
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HAT activity assay kit
Supplier: ENZO LIFE SCIENCES
Histone acetyltransferases (HATs) have been implicated to play a crucial role in various cellular functions, such as gene transcription, differentiation, and proliferation. HAT Activity Colorimetric Assay Kit offers a convenient, nonradioactive system for a rapid and sensitive detection of HAT activity in mammalian samples.
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Glycolysis Cell-Based Assay Kit
Supplier: Cayman Chemical
A colorimetric-based method for detecting L-lactate in culture medium.
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Phagocytosis assay kit (IgG FITC)
Supplier: Cayman Chemical
Phagocytosis Assay Kit (IgG FITC) for the identification of factors regulating the phagocytic process in vitro.
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Calcium assay kit
Supplier: Cayman Chemical
For the determination of total calcium concentration in a variety of biological samples, as well as tissue homogenates and cell lysates.
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Cytoplasmic membrane labelling kits, CellBrite™
Supplier: Biotium
CellBrite kits are designed to label cell cytoplasmic membranes with a fluorescent membrane dye pre-dissolved as an optimised staining solution. By combining CellBrite membrane stains, it is possible to label multiple cell populations with distinctive colours, a capability useful for the studies of cell-cell interactions.
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Ubiquitinylation kit
Supplier: ENZO LIFE SCIENCES
Versatile tool for generation of ubiquitin-E2 thioesters.
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Adipogenesis assay kit
Supplier: ENZO LIFE SCIENCES
Convenient method for the enhanced induction and detection of adipogenesis, in the classic 3T3L-1 model.
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Triglyceride colorimetric assay kit
Supplier: Cayman Chemical
Triglyceride Colorimetric Assay Kit is a simple, reproducible and sensitive tool for assaying triglycerides in plasma, serum, cell lysates and tissue homogenate samples.
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APO-BRDU™ kit, TUNEL Assay
Supplier: Tonbo Biosciences
The APO-DIRECT™ reagent kit provides a two color TUNEL (Terminal deoxynucleotide transferase dUTP Nick End Labelling) assay for labeling DNA breaks and total cellular DNA to detect apoptotic cells by flow cytometry. The kit contains the instructions and reagents required for measuring apoptosis in cells including: positive and negative control cells for assessing reagent performance; washing, reaction and rinsing buffers for processing individual steps in the assay; terminal deoxynucleotidyl tranferase enzyme (TdT), fluorescein isothiocyanate (FITC) deoxyuridine triphosphate (FITC-dUTP) for labeling DNA breaks and PI/RNase A solution for counter staining the total DNA.
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Calcium assay kit, FluoForte®
Supplier: ENZO LIFE SCIENCES
FluoForte® Calcium Assay Kits detect mobilisation of intracellular calcium utilising a fluorogenic calcium-binding dye optimised for superior cell-permeability and retention. The self-quenching dye undergoes an electronic change upon binding of calcium, resulting in a several order of magnitude greater fluorescence.
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ViaFluor® 488 live cell microtubule stain 1000X in DMSO
Supplier: Biotium
Stain microtubules in live cells with green fluorescence.
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Fluorescent nerve terminal probes and stains
Supplier: Biotium
Nerve terminal probes are a series of fluorescent cationic styryl dyes developed to follow synaptic activity. These dyes have a lipophilic tail at one end and a hydrophilic cationically charged head group at the other end. The dyes vary in the number of carbons in the lipophilic tail (m) and the number of double bonds linking the two aromatic rings in the dye (n). AM dyes are similar to FM dyes, but contain an additional formaldehyde-fixable amino group in the cationic head.
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Reagents for Color-de-Lys™ HDAC colorimetric activity assay kit
Supplier: ENZO LIFE SCIENCES
Cellular assay, Color-de-Lys™ deacetylase substrate, 50 µl
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LDH Cytotoxicity WST Assay
Supplier: ENZO LIFE SCIENCES
Colorimetric kit for the determination of cytotoxicity through the measurement of lactate dehydrogenase activity.
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sPLA2 activity kit
Supplier: ENZO LIFE SCIENCES
For the quantitative determination of sPLA2 in culture supernatants, cell lysates, and buffers from any species.
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Autophagy detection tests, CYTO-ID®
Supplier: ENZO LIFE SCIENCES
CYTO-ID can monitor autophagy in live cells and autophagic flux in lysosomally inhibited live cells using a novel dye that selectively labels accumulated autophagic vacuoles.
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CellBrite™ NIR cytoplasmic membrane dyes
Supplier: Biotium
Biotium’s CellBrite™ NIR Cytoplasmic Membrane Dyes are near-infrared lipophilic carbocyanine dyes for labeling cytoplasmic membranes in live or formaldehyde-fixed cells.
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Thiol Detection Assay Kits
Supplier: Cayman Chemical
A simple, reproducible, and sensitive tool for assaying free thiol content in samples.
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Cell viability assay kits
Supplier: Biotium
MTT, XTT, and resazurin are reduced by mitochondrial metabolic activity to yield coloured or fluorescent products, and thus are useful for and assaying cell viability and quantitating cell number. MTT and XTT are reduced to coloured formazin salts that can be measured by absorbance. MTT generates an insoluble formazin salt, requiring cell lysis before the absorbance can be measured, while XTT does not require cell lysis for measurement. Resazurin is a non-fluorescent blue dye that is reduced to the pink fluorescent compound resorufin, which can be measured by fluorescence or absorbance.
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Auto-Ubiquitinylation Kit
Supplier: ENZO LIFE SCIENCES
This E3 ligase auto-ubiquitinylation kit enables proteins to be tested for ubiquitin E3 ligase activity through assessment of their ability to under auto-ubiquitinylation. Utilizing the first three steps in the ubiquitin cascade the kit facilitates ubiquitinylation of known or putative E3 ligase enzymes followed by Western blot analysis using the highly sensitive reagents provided or using antibodies to the specific protein of interest (user supplied). A high integrity ubiquitin E3 ligase enzyme is also provided for use as a positive control.