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363 results for Protein Linking and Labeling Systems

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Protein Linking and Labeling Systems

Protein linking and labeling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colors, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labeling follows a simple protocol of reaction, separation, and then use.

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Carbohydrate coupling resin

Carbohydrate coupling resin

Supplier: G-Biosciences

Carbohydrate coupling resin is designed for the covalent immobilisation of carbohydrate containing molecules, including glycoproteins, to agarose beads. It is ideal for the coupling of polyclonal antibodies as it allows for the optimal orientation of the antibodies for affinity purification.

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CF® Dye BCN

CF® Dye BCN

Supplier: Biotium

CF® Dyes with a BCN group react with azides via a copper-free bioorthogonal reaction to label target molecules for surface staining of live cells or cell extracts. Membrane-permeant CF® Dye BCN conjugates for intracellular labelling reactions are also available.

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Tyramide amplification kit with HRP streptavidin and CF® dye or biotin tyramide

Supplier: Biotium

Kits for increasing immunofluorescence sensitivity using HRP-catalysed tyramide signal amplification with the choice of next-generation fluorescent CF® dyes or biotin.

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Tyramide amplification kit with HRP goat, anti-mouse and CF® dye or biotin tyramide

Supplier: Biotium

Kits for increasing immunofluorescence sensitivity using HRP-catalysed tyramide signal amplification with the choice of next-generation fluorescent CF® dyes or biotin.

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ExoBrite™ EV Surface Stain Sampler Kit

Supplier: Biotium

The ExoBrite™ EV Surface Stain Sampler Kit, Green was developed to offer each of Biotium’s ExoBrite™ EV Surface Stains (CTB, WGA, and Annexin V) for assessing which stain offers the best coverage for the EV samples of interest.

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Affibody® Imaging Agent, Negative Control

Supplier: Abcam

Product to be used as a negative control for ErbB 2 Affibody® Molecule Imaging Agent.

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CF®DI Aminooxy

Supplier: Biotium

CF®DI aminooxy dyes react with aldehyde/ketone groups on proteins or other biomolecules to form a stable oxime bond. The CF®DI dyes (CF®488DI, CF®555DI and CF®647DI) are designed to match each other for molecular weight and charge. The molecular weights of the same protein conjugated to different CF®DI dye colors are close to each other and the pl of the labeled proteins remains the same.

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CF™ dye picolyl azide

Supplier: Biotium

CF® dye picolyl azide reacts with alkyne to form 1,2,3-triazole by 1,3-dipolar Huisgen cycloaddition through the use of a much lower copper (I) concentration without sacrificing reaction efficiency. The selected CF® dyes are exceptionally bright and photostable, making them the perfect dye for fluorescence detection.

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Red 580 dUTP

Supplier: ENZO LIFE SCIENCES

Red 580 [5-ROX] dUTP can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase. Fluorescently labelled probes can be prepared with this fluorescent nucleotide by a variety of methods including nick translation, random prime labelling, cDNA labelling and 3’-end labelling. Probes generated by these methods are suitable for use for the identification of specific sequences by in situ hybridization procedures on fixed cells and tissues by direct fluorescence detection. Red 580 dUTP can also be used for multicolor fluorescence labelling.

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Aqua 431 dUTP

Aqua 431 dUTP

Supplier: ENZO LIFE SCIENCES

Aqua 431 [DEAC] dUTP is a coumarin derivative that can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase. Fluorescently labelled probes can be prepared with this fluorescent nucleotide by a variety of methods including nick translation, random prime labelling, cDNA labelling and 3’-end labelling. Probes generated by these methods are suitable for use for the identification of specific sequences by in situ hybridization procedures on fixed cells and tissues by direct fluorescence detection. Aqua 431 dUTP can also be used for multicolor fluorescence labelling.

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Bungarotoxins and fluorescent bungarotoxin conjugates

Bungarotoxins and fluorescent bungarotoxin conjugates

Supplier: Biotium

Bungarotoxins are venom toxins from the many-banded krait (Bungarus Multicinctus), which inhibit neurotransmission at the neuromuscular junction. Alpha-bungarotoxin is a potent inhibitor for the motor endplate acetylcholine receptor (Kd = 1 nM to 1 pM). Beta-bungarotoxin contains a small subunit that blocks voltage-gated K+ channels on the presynaptic membranes, and a large subunit that has phospholipase A2 activity. Fluorescent conjugates of alpha-bungarotoxin can be used to label acetylcholine receptors in skeletal muscle endplates.

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Red 580 dUTP (lyophilised)

Supplier: ENZO LIFE SCIENCES

Red 580 dUTP (lyophilised)

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D-luciferin luminescent substrates

Supplier: Biotium

Luciferin is a substrate for the enzyme firefly luciferase.

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Nanobody labelling kits, Mix-n-Stain™

Nanobody labelling kits, Mix-n-Stain™

Supplier: Biotium

The simplicity and convenience of Mix-n-Stain™ labelling of nanobodies with fluorescent CF® dyes or biotin.

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100-reaction single-round RNA amplification and Biotin labeling system

Supplier: ENZO LIFE SCIENCES

Improved data quality and analysis results through greater biotin incorporation.

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Photoreactive dyes and reagents

Supplier: Biotium

INA (5-iodonaphthyl-1-azide) is a lipophilic photoreactive probe, which has been used to selectively label membrane-embedded cysteine residues of proteins.

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Peroxidase anti-peroxidase (PAP) soluble complexes

Supplier: Rockland Immunochemicals

Peroxidase anti-peroxidase soluble complexes are used for peroxidase enzymatic labelling experiments.

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Reactive dye conjugates

Supplier: Biotium

Reactive dyes containing an amine functional group.

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BIOARRAY™ Low Input RNA Amplification with Biotin Labeling System

Supplier: ENZO LIFE SCIENCES

The BIOARRAY™ Low Input RNA Amplification with Biotin Labeling System provides an optimised protocol and reagents for the production of biotin-labeled antisense RNA (aRNA) from total cellular RNA samples in less than 24 hours for array analysis.

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Stabiliser for proteins and standards, SeramunStab® Stpur

Supplier: Apollo Scientific

Liquid product colourless to slightly yellow or greenish odourless pH 7,3 to 7,5 boiling point 104 °C. Stabiliser for controls and protein standards.

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Maackia amurensis Lectin (MAA/MAL II) (FITC)

Supplier: US Biological

Maackia amurensis Lectin (MAA/MAL II) is labelled with fluorescein isothiocyanate (FITC) and has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin.

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Small ligand labelling kits, Mix-n-Stain™ CF® Dye

Small ligand labelling kits, Mix-n-Stain™ CF® Dye

Supplier: Biotium

Easily label SNAP-tag®, CLIP-tag™, HaloTag®, or other ligands with fluorescent dyes, no purification needed. Cell membrane-permeant and impermeant dye options for intracellular or surface targets.

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HRP - conjugate stabiliser, SeramunStab® STM

Supplier: Apollo Scientific

Liquid product orange to red odourless pH 6,4 to 6,6. Stabiliser for conjugates with monomeric HRP. Preservation of very low conjugate concentrations.

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CF®DI Hydrazide

Supplier: Biotium

CF®DI dye hydrazides react with molecules containing aldehyde or ketone groups to form a hydrazone bond. The dyes are size- and charge-matched and do not significantly alter the molecular weight or pI of the labeled proteins. They are used for detection of protein carbonylation by fluorescence 2D-DIGE.

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CF® Dye Alkyne

CF® Dye Alkyne

Supplier: Biotium

CF® Dye alkynes react with azide groups via copper-catalysed bioorthogonal cycloaddition. Can be used to fluorescently detect or label azide groups on target molecules. CF® Dye alkynes can also be used as building blocks to form fluorescent polymers.

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CF® Reactive Dyes and Protein Labelling Kits

CF® Reactive Dyes and Protein Labelling Kits

Supplier: Biotium

Biotium offers a number of reactive dye formats for labelling proteins, nucleic acids, and other biomolecules with fluorescent dyes. CF® dyes offer advantages in brightness and photostability compared other similar commercial dyes. CF® dyes are available in a number of reactive dye formats for CF® dye colours span the visible and near-infrared spectra.

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Streptavidin, R-phycoerythrin (R-PE) conjugate

Supplier: Biotium

Streptavidin conjugates can be used to detect biotinylated molecules with extremely high sensitivity. They are commonly used as secondary reagents to detect biotinylated primary probes such as antibodies in flow cytometry, Western blotting, immunocytochemistry and other applications.

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Gold 525 dUTP

Supplier: ENZO LIFE SCIENCES

Gold 525 [5(6)-Carboxyrhodamine 6G] dUTP can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase. Fluorescently labelled probes can be prepared with this fluorescent nucleotide by a variety of methods including nick translation, random prime labelling, cDNA labelling and 3’-end labelling. Probes generated by these methods are suitable for use for the identification of specific sequences by in situ hybridization procedures on fixed cells and tissues by direct fluorescence detection. Gold 525 dUTP can also be used for multicolor fluorescence labelling.

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Immobilisation kit, SDC™ (Steroid/Drug/Compound)

Immobilisation kit, SDC™ (Steroid/Drug/Compound)

Supplier: G-Biosciences

SDC™ (Steroid/Drug/Compound) Immobilisation Kit is designed for the immobilisation of steroids, drugs and chemical compounds that lack primary amines, sulfhydryls, carbonyls and other common coupling groups to a solid-phase agarose support for the use in affinity purification. The kit uses Immobilised DADPA (diaminodipropylamine) resin to bind steroids, drugs and chemicals through their active hydrogens.

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Buccutite™ Rapid PerCP Antibody Labelling Kits (2 Labellings)

Buccutite™ Rapid PerCP Antibody Labelling Kits (2 Labellings)

Supplier: AAT BIOQUEST

PerCP (Peridinin-chlorophyll-protein complex) is isolated from Dinophyceae sp.

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