You searched for: Protein Linking and Labeling Systems

Supplier: G-Biosciences

HOOK™ d-Biotin (Vitamin H), 500 mg

Supplier: Biotium

Fluorescently labelled cAMP analogs that can be used to probe cAMP receptors.

Supplier: ENZO LIFE SCIENCES

Calcium dye

Supplier: Biotium

Biotium offers reactive biotin compounds with a variety of reactive functional groups.

Supplier: Columbia Biosciences

Many applications and instruments were developed specifically for R-phycoerythrin.

Supplier: Biotium

Coelenterazine and its analogs are luminescent enzyme substrates for apoaequorin and Renilla luciferase.

Supplier: Biotium

CF™ dye azide reacts with alkyne to form 1,2,3-triazole by copper(I) catalysed 1,3-dipolar Huisgen cycloaddition. It could also undergo Staudinger ligation with a phosphine containing compound.

Supplier: ENZO LIFE SCIENCES

The kit offers a set of six different MAbs specific for serine phosphorylation sites. Phosphorylation patterns in a given cell extract may differ when probed with different antibodies due to sequence specificity. Recognition therefore depends on two criteria: phosphorylation and surrounding aa motif. If one of the two criteria is not fulfilled, the antibody will not detect the phosphorylation site. Specificity has been determined by epitope mapping using degenerated phosphopeptide libraries. Clone 16B4 strongly interacts with pSK and pSP motifs, thus specifically recognizes substrates of MAP/SAP Kinases and CDC kinases.Clones 1C8, 4A3, 4A9, 4H4 and 7F12 interact with epitopes containing phosphoserine in the context of positive or neutral aa (e.g. substrates of PKA, PKB, PKC, PKG etc.) Though revealing a similar pattern in epitope mapping using phosphopeptide libraries, these antibodies interact with different sets of phosphoproteins in whole cell lysates.

Supplier: Biotium

Mix-n-Stain™ Biotin antibody labelling kits utilise a technology that allows rapid antibody labelling with biotin without a purification step.

Supplier: ENZO LIFE SCIENCES

Organelle-ID RGB® reagent IV is a mixture of cell-permeable red fluorescent endoplasmic reticulum dye, green fluorescent lysosomal dye, and blue fluorescent nucleic acid dye.

Supplier: ENZO LIFE SCIENCES

Mixture of fluorescent dyes for detection of lysosomes, endoplasmic reticulum and nucleic acids

Supplier: Biotium

CF™ dyes are superior to other fluorescent dyes for protein labelling due to advantages in brightness, photostability and water solubility.

Supplier: Biotium

An optimized buffer with enhanced sensitivity for tyramide signal amplification of immunostaining in cells or tissue sections.

Supplier: Biotium

Cholera toxin subunit B conjugated to Biotium's next-generation fluorescent CF™ dyes.

Supplier: Biotium

Mix-n-Stain™ FITC antibody labelling kits utilise a technology that allows rapid antibody labelling with green fluorescent dye (FITC) without a purification step.

Supplier: Biotium

Annexin V fluorescent conjugates can be used to detect apoptotic cells. Annexin V is available conjugated to a wide variety of dyes.

Supplier: ENZO LIFE SCIENCES

Bio-16-dUTP (Biotin-16-2’-deoxyuridine-5’-triphosphate) can replace TTP in reactions in which it serves as a substrate for E.coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase.

Supplier: Apollo Scientific

Liquid product yellow odourless pH-value 7,3 to 7,5 Boiling point 102 °C. Stabiliser for complex mixtures of biomolecules.

Supplier: Apollo Scientific

Liquid product red odourless pH-value 7,3 to 7,5 Boiling point 102 °C. Stabiliser for a broad range of HRP conjugates.

Supplier: Biotium

Dihydrorhodamine 123 is the reduced form of rhodamine 123, which is a commonly used fluorescent mitochondrial dye. It is non-fluorescent, but it readily enters most of the cells and is oxidized by oxidative species or by cellular redox systems to the fluorescent rhodamine 123 that accumulates in mitochondrial membranes. It is useful for detecting reactive oxygen species including superoxide (in the presence of peroxidase or cytochrome c) and peroxynitrite. Dihydrorhodamine 123 dihydrochloride is functionally equivalent to dihydrorhodamine 123 but with increased stability toward air oxidation and light during storage. Dihydroethidium (also called hydroethidium) is the chemically reduced form of the commonly used DNA dye ethidium bromide. The probe is useful to detect oxidative activities in viable cells, including respiratory burst in phagocytes. Dihydroethidium itself has blue fluorescence (355/420 nm) in cells, while the oxidized form ethidium has red fluorescence (518/605 nm) upon DNA intercalation. H2DCFDA (2′,7′-Dichlorodihydrofluorescein diacetate) is a useful fluorogenic reagent to detect reactive oxygen intermediates in cells. On oxidation, H2DCFDA becomes the highly green fluorescent 2′,7′-dichlorofluorescein, 504/529 nm (end product).

Supplier: Biotium

Fluorescent CF® Dye hydrazides are bright, extremely water-soluble, nontoxic molecules, useful as aldehyde-fixable cell tracers. They can also be used to label aldehyde or ketone groups of polysaccharides, carbohydrates or glycoproteins.

Supplier: Biotium

Lipophilic membrane dyes designed specifically for EV research and the best choice for general pan-EV staining.

Supplier: ENZO LIFE SCIENCES

Comparative genomic hybridisation (CGH) is a powerful diagnostic tool for detecting DNA copy number gains and losses associated with chromosome abnormalities. CGH provides an understanding of genetic disorders, cancers and other genomic aberrations.

Supplier: Apollo Scientific

Liquid product colourless to slightly yellow, freshly solutions opaleszent, odour weakly pH 7,3 to 7,5 boiling point 104 °C. Stabiliser for sensitive biomolecules like proteohormones or membrane proteins.

Supplier: ENZO LIFE SCIENCES

This kit provides the means of generating a thioester-linked NEDD8-conjugated E2 enzyme, utilizing the first two steps in the NEDD8 cascade, for use in the NEDDylation of E3 ligases and target substrate proteins (user supplied). A NEDD8-specific antibody is provided for detection of NEDDylated proteins via SDS-PAGE and Western blotting.

Supplier: G-Biosciences

Carboxyl coupling resin consists of 6% cross-linked agarose with covalently linked diaminodipropylamine (DADPA) to generate a free primary amine at the end of a long spacer arm. Molecules, including proteins, peptides and nucleic acids, are covalently coupled to the free primary amines, and the stable columns are ideal for affinity purification of antibodies and other interacting partners.

Supplier: Biotium

Kits for increasing immunofluorescence sensitivity using HRP-catalysed tyramide signal amplification with the choice of next-generation fluorescent CF® dyes or biotin.

Supplier: ENZO LIFE SCIENCES

Bio-N⁶-ddATP (Biotin-N⁶-(2’,3’-dideoxy)adenosine-5’ triphosphate) can be used for 3’-end labelling of single-stranded or double-stranded DNA. It is especially useful as a substrate for terminal transferase for the 3’-terminal addition of a single Bio-N⁶-ddAMP. It is also a substrate for Taq DNA Polymerase and other DNA polymerases.

Supplier: Rockland Immunochemicals

Avidin is biotin-binding protein, tetramer and suitable for used as a antigen, control and standard in assays. Avidin fluorescein, rhodamine, alkaline phosphatase, Texas Red™ conjugated are suitable for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent Western blotting. Avidin conjugated with glucose oxidase and peroxidase are useful as a detection reagent for primary antibodies conjugated with biotin and also can be utilised in Western blotting and ELISA experiments with proper substrates.

Supplier: Biotium

A wheat germ agglutinin (WGA) lectin labelled with Biotium’s CF® Dyes or HRP. It is a carbohydrate-binding lectin that has high affinity for sialic acid and N-acetylglucosamine moieties of glycoproteins. As a result, WGA conjugates will label yeast bud scars and the cell membrane of gram-positive bacteria and mammalian cells.