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Protein Linking and Labeling Systems

Protein Linking and Labeling Systems

Protein linking and labeling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colors, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labeling follows a simple protocol of reaction, separation, and then use.

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Biotin NTA

Biotin NTA

Supplier: Biotium

Biotinylated probes for detection of His-tagged proteins immobilised on nitrocellulose membranes.

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Amersham™ CyDye™ Value Packs (Bis-Reactive NHS Esters)

Amersham™ CyDye™ Value Packs (Bis-Reactive NHS Esters)

Supplier: Cytiva

Amersham™ CyDye™ fluors combine exceptional photostability with bright and intense signals, resulting in high sensitivity and a highly reproducible performance at a biologically suitable pH range of 3 to 10.

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Reactive dye 5-packs, Amersham CyDye™

Reactive dye 5-packs, Amersham CyDye™

Supplier: Cytiva

Mono-reactive dye pack contains derivatised CyDye™ that carries only one reactive group on each dye molecule for accurate labelling of amine groups, for example on antibodies or oligonucleotides.

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Amersham™ CyDye™ Value Packs (Mono-Reactive Hydrazide), Cytiva

Amersham™ CyDye™ Value Packs (Mono-Reactive Hydrazide), Cytiva

Supplier: Cytiva

Hydrazide esters provide a convenient method for the labelling of free carbonyl groups of glycoproteins and carbohydrates. For maximum convenience, stable active hydrazide esters are supplied and stored as solid material.

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Amersham™ CyDye™ Fluorescent Nucleotides

Amersham™ CyDye™ Fluorescent Nucleotides

Supplier: Cytiva

Fluorescent nucleotides for labelling DNA and RNA probes, especially during multi-colour analysis in microarrays, FISH, chromosome identification, whole chromosome painting, karyotyping, and gene mapping.

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Amersham Cy3 and Cy5 Maleimide Mono-Reactive Dye 5-Packs, Cytiva

Amersham Cy3 and Cy5 Maleimide Mono-Reactive Dye 5-Packs, Cytiva

Supplier: Cytiva

Mono-functional maleimides are particularly suitable for the selective labelling of molecules containing free sulfhydryl groups, such as cysteine residues in proteins and peptides and oligonucleotides.

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Protein labelling Kit, Amersham™ QuickStain

Protein labelling Kit, Amersham™ QuickStain

Supplier: Cytiva

The Amersham™ QuickStain Kit contains a Cy™5 fluorophore and labeling buffer for easy detection of proteins in SDSPAGE gels and on Western blotting membranes. The ready to use Cy5 N-hydroxysuccinimide (NHS) ester and Tris labeling buffer ensure robust and consistent labeling for detection of proteins in diverse samples.

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Fluorescent dyes, M series, streptavidin conjugate

Fluorescent dyes, M series, streptavidin conjugate

Supplier: Abnova

Fluorescent dye, M series, products are designed to be maximally excited by one of the major light sources equipped in flow cytometers. In comparison with phycobiliprotein tandems, Fluorescent dye, M series, possesses better photostability, higher conjugation yields, and little pH sensitivity making it an ideal choice in fluorescence imaging applications.

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Mono-Reactive NHS Ester dye packs, Amersham CyDye™

Mono-Reactive NHS Ester dye packs, Amersham CyDye™

Supplier: Cytiva

Mono-reactive dye pack contains derivatised CyDye™ that carries only one reactive group on each dye molecule for targeted and specific labelling of amine groups, for example on antibodies, peptides or oligonucleotides.

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Monofunctional maleimide fluors, CyDye™

Monofunctional maleimide fluors, CyDye™

Supplier: Cytiva

Cy3 and Cy5 monofunctional maleimides are particularly suitable for the selective labelling of molecules containing free sulfhydryl groups, such as cysteine residues in proteins and peptides and oligonucleotides.

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Amersham CyDye DIGE fluors

Amersham CyDye DIGE fluors

Supplier: Cytiva

CyDye™ DIGE fluors are available as minimal and saturation labeling dyes. The minimal dyes are intended for general 2-D application use where sufficient amounts of sample are available. The saturation dyes from the Scarce Sample Labeling Kit are designed to be used for applications where only small amounts of sample are available, for example in Laser Capture Microdissection.

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CyDye Antibody Labeling Kits

CyDye Antibody Labeling Kits

Supplier: Cytiva

CyDye Fluorescent Dyes are supplied as highly water soluble, reactive NHS esters for easy conjugation to biomolecules containing aliphatic amino groups.

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Labeling dye, Atto 488, Sigma-Aldrich®

Supplier: Merck

Atto 488 is a labeling dye with high molecular absorption (90000) and quantum yield (0,80) as well as sufficient stokes shift between excitation and emission maximum. It is optimised for excitation with an argon laser, and is characterised by high photostability.

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Atto 647N-biotin, BioReagent

Supplier: Merck

Designed for high sensitivity applications, including single molecule detection. These labels have rigid structures that do not show any cis-trans-isomerisation and display exceptional intensity with minimal spectral shift on conjugation.

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DNA labeling reagent, Atto 647 NHS Ester

Supplier: Merck

Atto 647 NHS ester is a reagent for the covalent labeling of molecules with Atto 647 flourescent dye.

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Peroxidase substrates, TrueBlue™

Supplier: SERACARE LIFE SCIENCES

KPL TrueBlue™ peroxidase substrate is a highly sensitive chromagenic substrate for visualisation of horseradish peroxidase-labeled reporter reagents.

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