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745 results for Buffers

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Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

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Fixation buffer for microscopy and flow cytometry

Fixation buffer for microscopy and flow cytometry

Supplier: Biotium

Ready-to-use formaldehyde-based fixation buffer for immunofluorescence staining for microscopy or flow cytometry.

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GET buffer

Supplier: Rockland Immunochemicals

GET buffer (50 mM Glucose, 10 mM EDTA, 25 mM Tris HCl, pH 8,0) is used in effective plasmid miniprep.

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ChIP Buffer, Abcam

Supplier: Abcam

ChIP Buffer for ab185913

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Buffer HF-Improved

Supplier: Thermo Fisher Scientific

Buffer used for pretreatment of planar silicon devices when plating with nickel plating solution products 44069 and 44070.

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Competent cell buffer

Supplier: Rockland Immunochemicals

This product is ready-to-use as a working 1X solution and requires no further dilution. This buffer consists of 60 mM Calcium Chloride, 15% v/v Glycerol, 10mM Tris HCl at a pH of 7,5.

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Buffer, EDTA buffer solution pH 8,5 (0,5 mol/L)

Supplier: G-Biosciences

A DNase and RNase free solution of 0.5M EDTA.

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VWR® Sodium Acetate Buffer 3,0 M Solution, pH 5,2

VWR® Sodium Acetate Buffer 3,0 M Solution, pH 5,2

Supplier: VWR Chemicals

With diethyl pyrocarbonate (DEPC).

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Buffers, potassium phosphate buffer (0,5 mol/L) solutions

Supplier: Cayman Chemical

Potassium phosphate buffer solution (0,5 mol/l), pH value: 7,0

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Blocking buffer, fish gelatin

Supplier: Biotium

Fish gelatin is commonly used in blocking solutions to minimise background noise in immunodetection procedures such as Western Blotting and immunostaining. Fish gelatin can be used to block non-specific binding sites on positively charged nylon or PVDF membranes or for blocking and dilution of antibodies during immunostaining procedures. Unlike BSA or milk, fish gelatin does not contain IgG or serum proteins that could cross-react with mammalian antibodies.

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G-Biosciences 10X Concentrated TE Buffers (Tris-EDTA Buffer)

G-Biosciences 10X Concentrated TE Buffers (Tris-EDTA Buffer)

Supplier: G-Biosciences

The tris buffering agent and EDTA metal chelating properties help protect DNA and RNA.

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6X GelRed prestain loading buffer with tracking dye

Supplier: Biotium

6X GelRed prestain loading buffers are gel loading buffers containing density agents, tracking dyes, and GelRed dye. GelRed is a sensitive, stable and environmentally safe fluorescent nucleic acid dyes designed to replace the highly toxic ethidium bromide (EtBr).

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VWR® Phosphate Buffered Saline (PBS), Low Phosphate, 10X

VWR® Phosphate Buffered Saline (PBS), Low Phosphate, 10X

Supplier: VWR Chemicals

Irradiated concentrate of PBS, suitable for use in washing cells after dilution.

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Wash buffer salts, lyophilised, Enzo Life Sciences

Supplier: Enzo Life Sciences

Wash Buffer Salts can be used in tissue preparations, immunohistochemical staining procedures and in assays that require HRP-linked detection reagents.

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VWR® AVS TITRINORM, Cesium chloride/lanthanum chloride buffer solution acc. to schinkel for atomic absorption spectroscopy (aas)

VWR® AVS TITRINORM, Cesium chloride/lanthanum chloride buffer solution acc. to schinkel for atomic absorption spectroscopy (aas)

Supplier: VWR Chemicals

This material is intended for use as an ionisation buffer for AAS

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Buffer, TBS solution 10X concentrate (TRIS buffered saline) pH 7,4

Supplier: Cayman Chemical

Formulation: Concentrated TBS (250 mM Tris-base; pH 7,4; 1,37 M sodium chloride and 27 mM KCl).

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10X Tris-EDTA buffer pH 9,0

Supplier: Novus Biologicals

10X Tris-EDTA buffer pH 9.0.

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VWR® PBS/Tween® 20, 20X, Blot Wash Buffer, UltraPure Grade

VWR® PBS/Tween® 20, 20X, Blot Wash Buffer, UltraPure Grade

Supplier: VWR Chemicals

PBS Tween® 20 blot wash buffer is a 20X concentration with 1% Tween® 20.

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Buffer, sodium acetate buffer solution pH 5,2 (3 mol/L)

Buffer, sodium acetate buffer solution pH 5,2 (3 mol/L)

Supplier: Rockland Immunochemicals

This product was aseptically filtered through a Millipore 0,22 µm filter into clean, pre-sterilised containers. The product was tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and was found to be negative for bacteria.

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VWR® AVS TITRINORM, Cesium chloride/aluminium nitrate buffer solution acc. to schuhknecht and schinkel for determination of na, k and li by flame aas
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VWR® Tris Buffer Solution, pH 7,4 (0,1 mol/L) for Biotechnology

VWR® Tris Buffer Solution, pH 7,4 (0,1 mol/L) for Biotechnology

Supplier: VWR Chemicals

Cell culture buffer, TRIS buffer solution pH 7,4 (0,1 mol/l)

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PBS and TBS buffers

PBS and TBS buffers

Supplier: Rockland Immunochemicals

Phosphate buffered and Tris buffered saline are suitable for multiple applications including biological diluent buffer for antibodies or other biologics. Also may be used as a wash buffer for immunological assays including western blot, immunohistochemistry, immunofluorescence microscopy, and ELISA. Other applications may require detergents or other additional components.

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VWR® Phosphate Buffered Saline (PBS) with 0,05% Tween®20

VWR® Phosphate Buffered Saline (PBS) with 0,05% Tween®20

Supplier: VWR Chemicals

Phosphate Buffered Saline (PBS) with 0,05% Tween® 20, pH 7,5 is a ready-to-use 1× concentration.

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VWR®, Hybridisation buffers with 50% formamide

VWR®, Hybridisation buffers with 50% formamide

Supplier: VWR Chemicals

Hybridisation cocktail offering blocking and hybridisation for optimised complimentary base pairing of DNA and RNA in Southern blot, Northern blot, dot blot, colony and in situ hybridisation.

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Cell lysis buffer, RIPA

Supplier: Enzo Life Sciences

The RIPA liquid cell lysis buffer composes of 50 mM TRIS-hydrogen chloride, pH 7,4, containing 150 mM sodium chloride, 1 mM EDTA, 1 mM EGTA, 1,2% Triton X-100, 0,5% sodium deoxycholate, and 0,1% SDS.

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Mini flip-top vial with 9 ml Butterfield's buffer

Mini flip-top vial with 9 ml Butterfield's buffer

Supplier: 3M Food Safety

The 3M™ Mini Flip-Top Vial with 9 ml Butterfield's Buffer is a leak-resistant plastic cylinder with a flip-open lid that seals securely for safe shipping and handling.

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Cell culture salts, salt solutions and buffers

Cell culture salts, salt solutions and buffers

Supplier: Biowest

These irrigating buffers and salt solutions are sterile, physiological balanced solutions intended for use in the maintenance of mammalian cells, where a chemically defined, balanced salt solution provides an environment that will maintain the structural and physiological integrity of cells in vitro. Applications include the intermediate steps for cultivation of cells, like washing, centrifugation, suspending and counting, as well as for many analytical methods or biochemical treatments. Principally these solutions should be used to keep the cells in suspension only for minutes or a few hours maximum. These buffers and solutions are not cell culture media.

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Buffers, TRIS HCl solutions, pH range 7,2 to 8,5 (1 mol/L)

Buffers, TRIS HCl solutions, pH range 7,2 to 8,5 (1 mol/L)

Supplier: Rockland Immunochemicals

Tris is widely used as a component of buffer solutions, such as in TAE and TBE buffer, especially for solutions of nucleic acids.

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In-situ hybridisation buffer (1.25X concentrate), Enzo Life Sciences

Supplier: Enzo Life Sciences

In Situ Hybridization Buffer provides a high quality hybridization medium for in situ hybridization analyses using either DNA or oligonucleotide probes labeled with biotin-, digoxigenin- or fluorescein-modified nucleotides.

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VWR® UltraPure, Buffer, PBS solution (phosphate buffered saline)

VWR® UltraPure, Buffer, PBS solution (phosphate buffered saline)

Supplier: VWR Chemicals

Dulbecco's formulation without calcium and magnesium.

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Tris-Borate-EDTA (TBE) buffer, 10X

Supplier: PanReac AppliChem

10X concentrated solution of 0,89 M Tris, 0,89 M boric acid and 0,02 M EDTA.Na₂, pH 8,3 ±0,2.

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