You searched for: Buffers

Supplier: Tonbo Biosciences

RBC Lysis Buffer (10X) is a concentrated ammonium chloride-based lysing reagent.

Supplier: Biotium

Ready-to-use permeabilisation buffer for intracellular immunofluorescence staining for microscopy or flow cytometry.

Supplier: G-Biosciences

The TBS solution is primarily used as a wash and blocking buffer diluent for Western blot, ELISA and immunoassays.

Supplier: ENZO LIFE SCIENCES

In situ hybridisation buffer for HPV probes (ready-to use) provides a high quality hybridisation medium for In situ hybridisation analyses using either DNA or oligonucleotide probes labeled with biotin-, or fluorescein-modified nucleotides.

Supplier: Rockland Immunochemicals

This product is ready-to-use as a working 1X solution and requires no further dilution. This buffer consists of 60 mM Calcium Chloride, 15% v/v Glycerol, 10mM Tris HCl at a pH of 7,5.

Supplier: G-Biosciences

The hybridisation buffers like Church & Gilbert’s, Denhardt solution, hybridisation denaturing solution, hybridisation neutralising solution, SSC, SSPE, TMAC Hyb solution and TNT Buffer are used in wide range of applications. Church & Gilbert’s Hybridization Buffer is prepared from molecular biology grade SDS (sodium dodecyl sulfate), monosodium phosphate (NaHPO₄), EDTA (ethylenediaminetetracetic acid) and bovine serum albumin (BSA) Fraction V using DNase, RNase and Protease-free water. It consists of 1mM EDTA, 1% BSA, 0,5M sodium phosphate monobasic, and 7% SDS (at a pH of 7,2).

Supplier: Biotium

Ready-to-use formaldehyde-based fixation buffer for immunofluorescence staining for microscopy or flow cytometry.

Supplier: ENZO LIFE SCIENCES

MMP-1 colorimetric assay buffer

Supplier: G-Biosciences

A DNase and RNase free solution of 0.5M EDTA.

Supplier: PanReac AppliChem

Powder mixture to prepare PBS buffer (10X) according to Dulbecco.

Supplier: ENZO LIFE SCIENCES

50mM potassium phosphate buffer, pH 6,5, 50 mM NaCl, 2 mM EDTA, 0,01% Triton X-100®, 0,5 mM DTT.

Supplier: VWR Chemicals

Kit contains 500 ml of pH 4,0, pH 7,0 and pH 10,0 standards.

Supplier: Biotium

6X GelRed prestain loading buffers are gel loading buffers containing density agents, tracking dyes, and GelRed dye. GelRed is a sensitive, stable and environmentally safe fluorescent nucleic acid dyes designed to replace the highly toxic ethidium bromide (EtBr).

Supplier: Cayman Chemical

Potassium phosphate buffer solution (0,5 mol/l), pH value: 9,0

Supplier: PanReac AppliChem

Tablets for preparation of 100 ml phosphate buffered saline (PBS).

Supplier: HyClone products (Cytiva)

Balanced salt solution used for multiple cell culture applications, (liquid form).

Supplier: Biotium

The TrueBlack® WB blocking buffer kit is a ready-to-use buffer system that provides optimal specificity, sensitivity, and background signal suppression for fluorescence-based western blotting (WB).

Supplier: ENZO LIFE SCIENCES

SIRT assay buffer

Supplier: Thermo Fisher Scientific

Cloning Buffer, Sodium acetate buffer solution pH 5,2 (3 mol/l)

Supplier: Novus Biologicals

10X Tris-EDTA buffer pH 9.0.

Supplier: G-Biosciences

The conjugation and modification reactions used to cross-link proteins or couple labels to proteins, such as biotin, enzymes, and fluorescent dyes, require certain conditions, including pH and chemical composition for optimal conjugation. Many common buffers routinely used in laboratories have an inhibitory effect on conjugation reactions; for example, Tris buffers inhibit coupling to amines.

Supplier: PanReac AppliChem

10X concentrated solution of 0,89 M Tris, 0,89 M boric acid and 0,02 M EDTA.Na₂, pH 8,3 ±0,2.

Supplier: G-Biosciences

Premixed buffer powders that simply require adding water to generate a ready-to-use buffer.

Supplier: Rockland Immunochemicals

This product is ready-to-use as a working 1X solution and requires no further dilution. This buffer consists of 50 mM Tris HCl, 10 mM magnesium sulphate at pH 7,5 and was prepared using ultra pure reagents dissolved in pharmaceutical grade deionised water.

Supplier: ENZO LIFE SCIENCES

In Situ Hybridization Buffer provides a high quality hybridization medium for in situ hybridization analyses using either DNA or oligonucleotide probes labeled with biotin-, digoxigenin- or fluorescein-modified nucleotides.

Supplier: ENZO LIFE SCIENCES

EGTA buffer, 2X

Supplier: Biowest

These irrigating buffers and salt solutions are sterile, physiological balanced solutions intended for use in the maintenance of mammalian cells, where a chemically defined, balanced salt solution provides an environment that will maintain the structural and physiological integrity of cells in vitro. Applications include the intermediate steps for cultivation of cells, like washing, centrifugation, suspending and counting, as well as for many analytical methods or biochemical treatments. Principally these solutions should be used to keep the cells in suspension only for minutes or a few hours maximum. These buffers and solutions are not cell culture media.

Supplier: ENZO LIFE SCIENCES

Cell lysis buffer 4, Enzo Life Sciences

Supplier: ENZO LIFE SCIENCES

PTP1B 2x assay buffer

Supplier: Rockland Immunochemicals

Concentrate diluent buffer for DAB membrane substrate, 10X