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You searched for: Cell Culture Supplements and Additives

Cell Culture Supplements and Additives

The tissue culture media supplements and additives compensate for characteristics the base medium lacks in providing maximum cell growth conditions. Incorporating the substances during development brings stability to the cultivated cell environments for maximum activity control. The stabilizing liquids added will support cell productivity, isolation, and microorganism identification. With a variety of tissue culture supplements and additives concentrations offered, a substance possessing the correct compatibility even with sensitive mediums is guaranteed.

Insulin-Transferrin-Selenium (ITS) 100x Growth Supplement, Corning®
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Phosphatase inhibitor cocktail I

Supplier: Thermo Fisher Scientific

Phosphatase Inhibitor Cocktail I is used to inhibitors with a broad specificity for serine, cysteine, and acid proteases, and aminopeptidases.

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MEM amino acids 50X solution without L-glutamine, Corning®

MEM amino acids 50X solution without L-glutamine, Corning®

Supplier: Corning

The addition of MEM nonessential amino acids to medium can improve growth and the viability of propagating cells in culture while reducing the biosynthetic burden on these cells.

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Corning® Matrigel® Basement Membrane Matrix, Corning

Corning® Matrigel® Basement Membrane Matrix, Corning

Supplier: Corning

Growth Factor Reduced (GFR) formulation is well suited for applications that require a more highly defined basement membrane preparation.

    
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Protease inhibitor cocktails I, animal-free

Supplier: Thermo Fisher Scientific

A 1X stock solution contains 500 μM AEBSF.HCl, 150 nM aprotinin, 1 μM E-64, 0,5 mM EDTA and 1 μM leupeptin hemisulfate. Reconstitute each vial with 1 ml water to obtain a 100X stock solution.

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Cell dissociation reagent, Corning® Cellstripper®

Cell dissociation reagent, Corning® Cellstripper®

Supplier: Corning

Cellstripper® is a non-enzymatic cell dissociation solution formulated with a proprietary mixture of chelators that gently dislodge adherent cells in tissue culture. It is an alternative to trypsin when used with either serum-free or serum-supplemented media. Because Cellstripper® is a gentler reagent than trypsin, cells can be exposed to Cellstripper for longer periods of time. Cellstripper® achieves superior results in comparison to enzymatic dissociation products.

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Corning® Nu-Serum™ Serum Replacements, Corning

Corning® Nu-Serum™ Serum Replacements, Corning

Supplier: Corning

Corning® Nu-Serum™ growth media supplement provides a low-protein alternative to newborn calf, foetal bovine, and other sera routinely used for cell culture. The low-protein content facilitates protein purification, virus production, monoclonal antibody production and screening, and increases the frequency of successful transfection of cells.

    
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Corning® Cell-Tak™ Cell and tissue adhesive

Corning® Cell-Tak™ Cell and tissue adhesive

Supplier: Corning

Corning® Cell-Tak™ Cell and Tissue Adhesive is a formulation of polyphenolic proteins extracted from Mytilus edulis (marine mussel). These proteins are the key components of the glue secreted by the mussel to anchor itself to solid substrates in the marine environment. Corning® Cell-Tak™ is used to attach cells or tissue sections to many types of surfaces, including plastic, glass, metal, PTFE, and biological materials. It can simplify the manipulation of biological samples for a number of in vitro techniques, including in situ hybridisation, immunoassays, microinjection, immunohistochemistry, and establishing primary cells in culture. Corning® Cell-Tak™ is biocompatible and demonstrates no species specificity.

    
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Trace element solution 2, MP Biomedicals

Supplier: MP Biomedicals

Trace Element Solution 2 is a 1000X sterile filtered solution, suitable for use with serum-free media; ideal for plant cell culture.

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Cell detachment solution, Accutase, MP Biomedicals

Supplier: MP Biomedicals

Accutase solution is a trypsin replacement that contains both proteolytic and collagenolytic enzymes for cell detachment.

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Cell culture supplement, Basal Medium Eagle (BME) Vitamin Concentrate (100X), MP Biomedicals

Supplier: MP Biomedicals

Basal Medium Eagle (BME) Vitamin Concentrate (100X), without L-glutamine

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Polyribonucleotide poly(I)·poly(C)

Polyribonucleotide poly(I)·poly(C)

Supplier: Cytiva

The double-stranded polyribonucleotide poly(I)·poly(C) induces interferon in rabbit cells, and there has been interest in its effectiveness for inducing human interferon in vitro.

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Middlebrook 7H10 OADC (Oleic Albumin Dextrose Catalase) Growth Supplement

Supplier: US Biological

Enrichment supplement recommended for the cultivation of Mycobacteria sp. OADC enrichment medium is used with Middlebrook 7H10 Mycobacteria Agar (Powder).

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Protease inhibitor cocktail, MP Biomedicals

Supplier: MP Biomedicals

A broad spectrum protease inhibitor cocktail which covers both endopeptidases and exopeptidases, non-specific and specific. The cocktail consists of four major protease inhibitors such as AEBSF, Sodium EDTA, Leupeptin and Pepstain A.

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Protease inhibitor mix

Protease inhibitor mix

Supplier: Cytiva

Sample preparation often requires the inhibition of protease activity. Protease inhibitor mix combines competitive and non competitive protease inhibitors, which protect proteins from proteolysis during purification from animal tissues, plant tissues, yeast and bacteria.

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FastGro synthetic, animal free, chemically defined FBS replacement, sterile, MP Biomedicals

FastGro synthetic, animal free, chemically defined FBS replacement, sterile, MP Biomedicals

Supplier: MP Biomedicals

FastGro allows culturing cells in vitro without the use of serum and without any animal or human derived compound. FastGro is a fully defined additive, avoiding the need for serum, for a wide range of cell culture practices. Since FastGro is chemically defined it avoids lot-to-lot variations. All components in FastGro are highly purified and identified chemical compounds.

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BIOFLOAT™ FLEX Coating Solution

BIOFLOAT™ FLEX Coating Solution

Supplier: FACELLITATE

The BIOFLOAT™ FLEX coating solution guarantees the creation of surfaces without unspecific protein or cell binding.

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Epidermal growth factor (EGF) from E.coli, MP Biomedicals

Supplier: MP Biomedicals

Epidermal Growth Factor (EGF) is a polypeptide growth factor which stimulates the proliferation of a wide range of epidermal and epithelial cells.

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Brewers yeast, MP Biomedicals

Brewers yeast, MP Biomedicals

Supplier: MP Biomedicals

Brewers yeast is often used as a source of B-complex vitamins, chromium, and selenium. It is also can be used to bake bread, ferment wine, beer and ethanol production.

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Lymphocyte Separation Medium

Lymphocyte Separation Medium

Supplier: Tonbo Biosciences

Lymphocyte Separation Medium (LSM) is a sterile-filtered iso-osmotic solution designed for the in vitro isolation of lymphocytes from diluted whole blood.

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MEM Eagle non-essential amino acids (NEAA) concentrate (100X solution), MP Biomedicals

Supplier: MP Biomedicals

Most cells can synthesise non-essential amino acids (NEAA) through glucoaminolysis, glycolysis, or the TCA cycle. When these amino acids concentration levels are low, cells need to consume more glucose and glutamine in the media and produce excess by-products, which might affect cell growth. Adding NEAA into the cell culture medium could mitigate the potential side effects caused by self-production of these non-essential amino acids.

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Egg yolk tellurite emulsion for microbiology, Millipore®

Supplier: Merck

Sterile stabilized tellurite emulsion of egg yolk recommended for the identification of Staphylococcus species.

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Endothelial Cell Growth Medium MV2

Supplier: Merck

Endothelial cell growth medium MV2 was developed for the in vitro cultivation of endothelial cells from microvascular vessels, the coronary artery, and the aorta.

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Cell detachment solution, Accumax™

Supplier: Merck

Accumax™ is a cell detachment solution consisting of proteolytic and collagenolytic enzymes.

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Egg yolk emulsion for microbiology, Millipore®

Supplier: Merck

Sterile stabilized emulsion of egg yolk recommended for use in various culture media.

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Sigma-Aldrich® Select yeast extract, BioReagent, suitable for cell culture

Supplier: Merck

Select Yeast extract is used as a bacteriological media component for a variety of microorganisms and molecular genetics applications.

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Plant Tissue Culture System Temporary Immersion

Supplier: Merck

Nourish and oxygenate plant cultures by intermittent immersion in medium with the easy to use RITA® system.

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Rat tail collagen coating solution

Supplier: Merck

Rat tail collagen coating solution enhances cell attachment and proliferation, or promotion of cell-specific morphology and function. Commonly used for endothelial cells, hepatocytes, muscle cells, and a variety of other cell types. This collagen solution is prepared in 0,02 M acetic acid at 50 ug/ml and sterile filtered.

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