You searched for: Cell Culture Supplements and Additives
The tissue culture media supplements and additives compensate for characteristics the base medium lacks in providing maximum cell growth conditions. Incorporating the substances during development brings stability to the cultivated cell environments for maximum activity control. The stabilizing liquids added will support cell productivity, isolation, and microorganism identification. With a variety of tissue culture supplements and additives concentrations offered, a substance possessing the correct compatibility even with sensitive mediums is guaranteed.
Insulin-Transferrin-Selenium (ITS) 100x Growth Supplement, Corning®
Supplier: Corning
Animal free formulation
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Phosphatase inhibitor cocktail I
Supplier: Thermo Fisher Scientific
Phosphatase Inhibitor Cocktail I is used to inhibitors with a broad specificity for serine, cysteine, and acid proteases, and aminopeptidases.
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MEM amino acids 50X solution without L-glutamine, Corning®
Supplier: Corning
The addition of MEM nonessential amino acids to medium can improve growth and the viability of propagating cells in culture while reducing the biosynthetic burden on these cells.
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Corning® Matrigel® Basement Membrane Matrix, Corning
Supplier: Corning
Growth Factor Reduced (GFR) formulation is well suited for applications that require a more highly defined basement membrane preparation.
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Protease inhibitor cocktails I, animal-free
Supplier: Thermo Fisher Scientific
A 1X stock solution contains 500 μM AEBSF.HCl, 150 nM aprotinin, 1 μM E-64, 0,5 mM EDTA and 1 μM leupeptin hemisulfate. Reconstitute each vial with 1 ml water to obtain a 100X stock solution.
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Cell dissociation reagent, Corning® Cellstripper®
Supplier: Corning
Cellstripper® is a non-enzymatic cell dissociation solution formulated with a proprietary mixture of chelators that gently dislodge adherent cells in tissue culture. It is an alternative to trypsin when used with either serum-free or serum-supplemented media. Because Cellstripper® is a gentler reagent than trypsin, cells can be exposed to Cellstripper for longer periods of time. Cellstripper® achieves superior results in comparison to enzymatic dissociation products.
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Corning® Nu-Serum™ Serum Replacements, Corning
Supplier: Corning
Corning® Nu-Serum™ growth media supplement provides a low-protein alternative to newborn calf, foetal bovine, and other sera routinely used for cell culture. The low-protein content facilitates protein purification, virus production, monoclonal antibody production and screening, and increases the frequency of successful transfection of cells.
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Corning® Cell-Tak™ Cell and tissue adhesive
Supplier: Corning
Corning® Cell-Tak™ Cell and Tissue Adhesive is a formulation of polyphenolic proteins extracted from Mytilus edulis (marine mussel). These proteins are the key components of the glue secreted by the mussel to anchor itself to solid substrates in the marine environment. Corning® Cell-Tak™ is used to attach cells or tissue sections to many types of surfaces, including plastic, glass, metal, PTFE, and biological materials. It can simplify the manipulation of biological samples for a number of in vitro techniques, including in situ hybridisation, immunoassays, microinjection, immunohistochemistry, and establishing primary cells in culture. Corning® Cell-Tak™ is biocompatible and demonstrates no species specificity.
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Trace element solution 2, MP Biomedicals
Supplier: MP Biomedicals
Trace Element Solution 2 is a 1000X sterile filtered solution, suitable for use with serum-free media; ideal for plant cell culture.
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Cell detachment solution, Accutase, MP Biomedicals
Supplier: MP Biomedicals
Accutase solution is a trypsin replacement that contains both proteolytic and collagenolytic enzymes for cell detachment.
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Cell culture supplement, Basal Medium Eagle (BME) Vitamin Concentrate (100X), MP Biomedicals
Supplier: MP Biomedicals
Basal Medium Eagle (BME) Vitamin Concentrate (100X), without L-glutamine
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Polyribonucleotide poly(I)·poly(C)
Supplier: Cytiva
The double-stranded polyribonucleotide poly(I)·poly(C) induces interferon in rabbit cells, and there has been interest in its effectiveness for inducing human interferon in vitro.
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Middlebrook 7H10 OADC (Oleic Albumin Dextrose Catalase) Growth Supplement
Supplier: US Biological
Enrichment supplement recommended for the cultivation of Mycobacteria sp. OADC enrichment medium is used with Middlebrook 7H10 Mycobacteria Agar (Powder).
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Protease inhibitor cocktail, MP Biomedicals
Supplier: MP Biomedicals
A broad spectrum protease inhibitor cocktail which covers both endopeptidases and exopeptidases, non-specific and specific. The cocktail consists of four major protease inhibitors such as AEBSF, Sodium EDTA, Leupeptin and Pepstain A.
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Protease inhibitor mix
Supplier: Cytiva
Sample preparation often requires the inhibition of protease activity. Protease inhibitor mix combines competitive and non competitive protease inhibitors, which protect proteins from proteolysis during purification from animal tissues, plant tissues, yeast and bacteria.
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FastGro synthetic, animal free, chemically defined FBS replacement, sterile, MP Biomedicals
Supplier: MP Biomedicals
FastGro allows culturing cells in vitro without the use of serum and without any animal or human derived compound. FastGro is a fully defined additive, avoiding the need for serum, for a wide range of cell culture practices. Since FastGro is chemically defined it avoids lot-to-lot variations. All components in FastGro are highly purified and identified chemical compounds.
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BIOFLOAT™ FLEX Coating Solution
Supplier: FACELLITATE
The BIOFLOAT™ FLEX coating solution guarantees the creation of surfaces without unspecific protein or cell binding.
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Epidermal growth factor (EGF) from E.coli, MP Biomedicals
Supplier: MP Biomedicals
Epidermal Growth Factor (EGF) is a polypeptide growth factor which stimulates the proliferation of a wide range of epidermal and epithelial cells.
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Brewers yeast, MP Biomedicals
Supplier: MP Biomedicals
Brewers yeast is often used as a source of B-complex vitamins, chromium, and selenium. It is also can be used to bake bread, ferment wine, beer and ethanol production.
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Lymphocyte Separation Medium
Supplier: Tonbo Biosciences
Lymphocyte Separation Medium (LSM) is a sterile-filtered iso-osmotic solution designed for the in vitro isolation of lymphocytes from diluted whole blood.
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MEM Eagle non-essential amino acids (NEAA) concentrate (100X solution), MP Biomedicals
Supplier: MP Biomedicals
Most cells can synthesise non-essential amino acids (NEAA) through glucoaminolysis, glycolysis, or the TCA cycle. When these amino acids concentration levels are low, cells need to consume more glucose and glutamine in the media and produce excess by-products, which might affect cell growth. Adding NEAA into the cell culture medium could mitigate the potential side effects caused by self-production of these non-essential amino acids.
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Egg yolk tellurite emulsion for microbiology, Millipore®
Supplier: Merck
Sterile stabilized tellurite emulsion of egg yolk recommended for the identification of Staphylococcus species.
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Endothelial Cell Growth Medium MV2
Supplier: Merck
Endothelial cell growth medium MV2 was developed for the in vitro cultivation of endothelial cells from microvascular vessels, the coronary artery, and the aorta.
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Cell detachment solution, Accumax™
Supplier: Merck
Accumax™ is a cell detachment solution consisting of proteolytic and collagenolytic enzymes.
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Egg yolk emulsion for microbiology, Millipore®
Supplier: Merck
Sterile stabilized emulsion of egg yolk recommended for use in various culture media.
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Sigma-Aldrich® Select yeast extract, BioReagent, suitable for cell culture
Supplier: Merck
Select Yeast extract is used as a bacteriological media component for a variety of microorganisms and molecular genetics applications.
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Plant Tissue Culture System Temporary Immersion
Supplier: Merck
Nourish and oxygenate plant cultures by intermittent immersion in medium with the easy to use RITA® system.
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Rat tail collagen coating solution
Supplier: Merck
Rat tail collagen coating solution enhances cell attachment and proliferation, or promotion of cell-specific morphology and function. Commonly used for endothelial cells, hepatocytes, muscle cells, and a variety of other cell types. This collagen solution is prepared in 0,02 M acetic acid at 50 ug/ml and sterile filtered.