You searched for: Extraction Buffers
Extraction buffers are provided ready to use and are effective in the isolation of total RNA and other proteins, improving protein yields and sample complexity from cells and tissues. Optimized extraction reagents enhance protein extraction in native or denaturing buffers compatible with downstream analytical techniques. An appropriate buffer solution added to a protein mixture during the extraction process can help improve the stability of protein molecules as these molecules are subjected to various forces designed to isolate them for study.
HEPES protein extraction buffer (5X)
Supplier: Thermo Fisher Scientific
Thermo Fisher Scientific
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ReadiUse™ Yeast Cell Lysis Buffer, 5X
Supplier: AAT Bioquest
ReadiUse™ Yeast Cell Lysis Buffer is a biochemical reagent designed for efficient yeast cell lysis and protein solubilisation in laboratory applications.
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ReadiUse™ Bacterial Cell Lysis Buffer *5X* (10 ml)
Supplier: AAT Bioquest
Cell lysis refers to the breaking down of cells, and it is often used to analyze specific cellular compositions such as proteins, lipids, nucleic acids, reporter molecules, cell signal molecules and other small biomolecules.
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Y-PER™ Yeast Protein Extraction Reagent
Supplier: Thermo Fisher Scientific
This reagent uses a mild detergent lysis procedure to efficiently release functionally active solubilised proteins.
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Subcellular protein fractionation kit for tissues
Supplier: Thermo Fisher Scientific
This kit is ideal for cell fractionation and enrichment of proteins from five different cellular compartments within two hours.
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Protein extraction kit, Insect PE LB™
Supplier: G-Biosciences
Insect PE LB™ has been developed for extraction of total biologically active, soluble proteins from adherent or suspension cultured insect cells, including Sf9 and Sf21. Insect PE LB™ utilises a mild non-ionic detergent and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. The Insect PE LB™ is fully compatible with downstream processes, such as electrophoresis and chromatography. Depending on the required downstream application, additional agents such as reducing agents and protease inhibitors may be added into Insect PE LB™.
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Mammalian protein extract reagents, M-PER™
Supplier: Thermo Fisher Scientific
The M-PER Mammalian Protein Extraction Reagent is designed to provide highly efficient total protein extraction from cultured mammalian cells.
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Y-PER™ plus Yeast Protein Extraction Reagent, Dialysable
Supplier: Thermo Fisher Scientific
This extraction reagent is formulated to effectively lyse yeast and bacterial cells to extract and solubilise proteins for analysis.
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ReadiUse™ Mammalian Cell Lysis Buffer *5X*
Supplier: AAT Bioquest
All our ReadiUse™ reagents require minimal hands-on time.
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Nuclear and cytoplasmic extraction reagents, NE-PER
Supplier: Thermo Fisher Scientific
The NE-PER Nuclear and Cytoplasmic Extraction Kit provides for efficient cell lysis and extraction of separate cytoplasmic and nuclear protein fractions in less than two hours. The nuclear protein extraction method involves simple, stepwise lysis of cells and centrifugal isolation of nuclear and cytoplasmic protein fractions. A benchtop microcentrifuge, tubes and pipettors are the only tools required. The NE-PER Reagents efficiently solubilise and separate cytoplasmic and nuclear proteins into fractions with minimal cross-contamination or interference from genomic DNA and mRNA. The isolated proteins can be used to perform immunoassays as well as protein interaction studies such as mobility shift assays (EMSA), co-immunoprecipitation (Co-IP) and pull-down assays.
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Protein extraction reagent, BugBuster® 10X, Millipore®
Supplier: Merck Millipore (Novagen)
BugBuster® 10X is a concentrated formulation of the proprietary detergents employed in BugBuster® without the addition of salts or buffer components. Concentrated BugBuster® provides a flexible alternative to the ready-to-use standard 1X BugBuster®, allowing user-defined dilution and addition of buffer components. BugBuster® 10X has all of the bioprocessing benefits of standard BugBuster® plus the freedom to control pH, reagent concentration, and buffer additives necessary for maximum extraction and activity of your target protein.
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CHAPS lysis buffer
Supplier: Thermo Fisher Scientific
For cell lysis and protein extraction.
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Denaturing lysis buffer
Supplier: Thermo Fisher Scientific
Denaturing lysis buffer, Thermo Fisher Scientific
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RBC lysis buffer for mouse
Supplier: Thermo Fisher Scientific
RBC lysis buffer for mouse, Thermo Fisher Scientific
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HEPES lysis buffer with Triton® X-100
Supplier: Thermo Fisher Scientific
HEPES lysis buffer with Triton® X-100, Thermo Fisher Scientific
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Lysis buffer, 0,5% Tween 20, pH 8,0, for isolation of His-tag modified proteins
Supplier: Thermo Fisher Scientific
Lysis buffer, 0.5% Tween 20, pH 8.0, for isolation of His-tag modified proteins, Thermo Fisher Scientific
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NP-40 lysis buffer
Supplier: Thermo Fisher Scientific
NP-40 lysis buffer, Thermo Fisher Scientific
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Triton® X-100 lysis buffer, pH 7,4
Supplier: Thermo Fisher Scientific
Triton® X-100 lysis buffer, pH 7.4, Thermo Fisher Scientific
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NP-40 lysis buffer with glycerol (2X)
Supplier: Thermo Fisher Scientific
NP-40 lysis buffer with glycerol (2X), Thermo Fisher Scientific
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Cell lysis buffers
Supplier: Rockland Immunochemicals
Cell lysis buffer is used to lyse cells under non-denaturing conditions.
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MNE buffer, pH 6,5
Supplier: Thermo Fisher Scientific
MNE buffer, pH 6.5, Thermo Fisher Scientific
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NP-40 lysis buffer, low salt
Supplier: Thermo Fisher Scientific
NP-40 lysis buffer, low salt, Thermo Fisher Scientific
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HEPES lysis buffer with NP-40 (2X)
Supplier: Thermo Fisher Scientific
HEPES lysis buffer with NP-40 (2X), Thermo Fisher Scientific
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G-Biosciences ACD (Acid Citrate Dextrose) Solutions for Anticoagulants
Supplier: G-Biosciences
ACD (Acid Citrate Dextrose) Solution A and ACD (Acid Citrate Dextrose) Solution B are anticoagulants for whole blood and also act as acidifying agents to platelet poor plasma.
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NP-40 lysis buffer (2X)
Supplier: Thermo Fisher Scientific
NP-40 lysis buffer (2X), Thermo Fisher Scientific
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MES lysis buffer with Triton® X-100
Supplier: Thermo Fisher Scientific
MES lysis buffer with Triton® X-100, Thermo Fisher Scientific
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PIPES lysis buffer with NP-40 (2X)
Supplier: Thermo Fisher Scientific
PIPES lysis buffer with NP-40 (2X), Thermo Fisher Scientific
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Lysis buffers, red blood cells
Supplier: G-Biosciences
These DNase- and RNase-free buffers and reagents are designed for high quality research.