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Supplier: G-Biosciences

Prepacked purification columns for protein purification. Supplied in packs of five.

Supplier: G-Biosciences

Determination of protein concentration is an essential technique in all aspects of protein studies and proteomics. The Assays for Protein Quantification kit includes three of the most widely used protein assays and allows for a direct comparison of the three assays that teaches students the benefits and limitations of each assay. Each assay is available individually to allow teaching of a specific assay, without the option of comparing and contrasting with other assays. The three assays covered are the Biuret Protein Assay, Lowry Protein Assay and the Coomassie Blue Dye Protein Assay. The Assays for Protein Quantification kit provides all the reagents required to perform both protein assays, including protein standards for accurate quantification, in a single lab activity. An often underestimated factor in quantifying protein is the presence of non-protein interfering agents, such as salts and detergents. This kit teaches students about common laboratory agents that affect the protein assays, the reasoning behind their interferences and how to overcome the interference. Students also learn how to select a protein assay for different applications.

Supplier: G-Biosciences

This lab activity has two objectives - to deepen the understanding of protein molecules and for students to learn the potential of electrophoresis in protein analysis. Students study the fundamentals of protein structure from their primary structure to the more complex tertiary and quaternary structures, utilising protein electrophoresis. Complex mixtures of protein samples and characterised pure protein samples, in conjunction with electrophoresis, are utilised to study protein structure and the potential of protein electrophoresis. Using non-denaturing and denaturing electrophoresis, students understand the difference between primary, tertiary and quaternary structures, the importance of disulfhydryl bridges in maintaining protein structure and electrophoresis in studying complete proteins and protein subunits.

Supplier: G-Biosciences

A hands on lab activity to study immunoaffinity chromatography and use a specific antibody to purify antigenic proteins from complex samples. This technique involves performing a chromatography procedure using antigen or antibody immobilised on a chromatographic resin. The solution containing antigen or antibody is passed through the column, which specifically and efficiently captures antibodies (antigen). The captured molecules are eluted from the column as a pure fraction. In this lab activity, students perform immunoaffinity chromatography and learn how this method is utilised in research laboratories.

Supplier: G-Biosciences

The kit utilises the principle of diffusion of nucleic acids on a nylon membrane to determine their concentration. No spectrophotometers required.

Supplier: MACHEREY-NAGEL

An ideal introductory kit for the science education sector. The kit contains all the the chemicals, plates and accessories for students to perform separations of dyes including: anthaquinone dye mixtures, lipophilic dye mixtures and food dye mixtures. The TLC plates are polyester sheet based with either silica, cellulose or alumina as coating.

Supplier: G-Biosciences

The final goal in molecular biology is often the expression of a recombinant protein. The transformed plasmids can be used as templates by the bacteria to produce protein. Students learn about essential promoters and other elements necessary for successful protein expression in bacteria, including the differences between inducible and constitutive (unregulated) expression.The Expression of a Recombinant Protein kit allows students to express a protein either constitutively or under the control of an inducible promoter, which is activated with IPTG (isopropyl-beta-D-thiogalactopyranoside).

Supplier: G-Biosciences

A major challenge for molecular biologists and genetic engineers is to easily detect and analyze genetic mutations that occur naturally, causing diseases, or during genetic engineering or cloning, whether deliberate or accidental. Students learn about different types of genetic mutations, including substitutions, deletions and point mutations and about various techniques used to detect genetic mutations. The kit contains all the reagents to screen simulated clinical samples for a mutant gene, using both the polymerase chain reaction (PCR) and restriction digestion mapping. Students conduct a simple clinical diagnostic experiment in order to identify a diseased patient.

Supplier: MACHEREY-NAGEL

Advanced kit for science education.

Supplier: MACHEREY-NAGEL

Advanced kit for science education.

Supplier: G-Biosciences

Biodiversity lab activity is designed for determination of protein contents and biomass in diverse biological samples to study how biomass is related to biodiversity in nature. Students collect and catalogue plant leaf samples from a diverse group of locally available plants. This lab activity involves determination of natural weight of each plant sample, grinding a predetermined amount of each sample, and the subsequent extraction of proteins from the samples. Students then learn to determine protein contents of each plant sample and attempt to relate the protein content with biomass. Students in this lab activity are challenged to think, analyse, and seek answers as to why protein biomasses vary for a given natural weight for different plants. Finally, they will relate that finding to the biodiversity of nature.

Supplier: MACHEREY-NAGEL

Advanced kit for science education.

Supplier: EDVOTEK

Educational Materials, Microbiology, Application: Microbiology

Supplier: G-Biosciences

The Size Exclusion Chromatography kit teaches gel filtration or size exclusion chromatography and the use of this method in purification of biological samples. This method is based on separation of protein molecules based on their molecular size. This lab activity involves running size exclusion chromatography for separation of molecules based on their molecular sizes.

Supplier: G-Biosciences

Ion exchange chromatography is used to separate charged molecules from complex biological samples. The charged molecules bind to a solid support carrying an opposite charge to the molecule. Proteins contain regions of charged groups on the surface which interact with the ion exchange groups immobilised on the solid support (resin column). Immobilised proteins are eluted by changing either pH or the salt gradient or a combination of both. This lab activity involves preparation of a crude protein extract and running ion exchange chromatography for isolation of proteins.

Supplier: G-Biosciences

This lab activity is designed to study highly specific lock-key matching properties of antigen-antibody and how this highly specific interaction can be exploited as a tool for research and analysis. This study involves the use of an immunodiffusion technique in which antigen and antibody are allowed to diffuse in a solid agarose medium. When antigen and antibody meet, antigen-antibody complex is formed, which leads to precipitation. Antigen-antibody precipitate is formed in the zone where the concentration of the two matching pairs reaches an optimal known as the zone of equivalence, which results in formation of a visible opaque precipitate region in agarose medium. Those regions of precipitation can be used for determination of concentration or titer of both antigen and antibody. This Antigen-Antibody Interaction kit is a hands-on study of both Ouchterlony Double Diffusion and Radial Immunodiffusion techniques. This kit also provides additional guidance materials for teaching other types of antigen-antibody interactions concepts such as immunoelectrophoresis and immunoprecipitation.

Supplier: EDVOTEK

Educational Materials, Microbiology, Application: Microbiology, ReadyPour™ LB agar with ampicillin

Supplier: DIDALAB

Educational Materials, Optics, Application: Physics, Polariser, calibrated, set in an M49 ring

Supplier: DIDALAB

Educational Materials, Optics, Application: Physics, Diode laser, blue, Class II

Supplier: Thermo Fisher Scientific

Replace metal-type troughs with the polypropylene pneumatic trough.

Supplier: DIDALAB

Educational Materials, Optics, Application: Physics, Translucent screen 20×20 cm, attached to a rod with Ø 10 mm

Supplier: EDVOTEK

Link important STEM concepts using Agarose Gel Electrophoresis.

Supplier: DIDALAB

The diameter of the beam is adjustable from 1 to 12 mm.

Supplier: G-Biosciences

The Gram staining method was first described in 1844 by the Danish bacteriologist Hans Christian Gram, after whom the test was named. The Gram staining test for bacteria is one of the most important tests in microbiology and is often one of the first tests performed in the identification of bacteria. The Gram staining method utilises the properties of bacterial cell walls and the stain crystal violet. Gram-positive microorganisms have a higher peptidoglycan and lower lipid content than Gram-negative bacteria in their cell walls and this makes them capable of retaining the Gram stain. This kit is supplied with two strains of bacteria and all the necessary components to carry out the Gram staining.

Supplier: DIDALAB

Economical, recent design of prismatic optical bench

Supplier: DIDALAB

Extremely luminous beam, ideal for classroom experiments. Class IIIa (5 mW Power)

Supplier: G-Biosciences

This kit teaches aseptic handling techniques and cultivation of bacteria in liquid culture media and on solid phase agar plates. This kit is designed to educate students about the various stages of the bacterial growth cycle, i.e. lag, log or exponential, stationary and decline or death phases. The kit also teaches the importance of growing bacteria on solid phase agar plates to isolate single colonies of bacteria. This lab activity involves preparation of culture medium and solid agar plates.

Supplier: G-Biosciences

Antibiotics play a crucial role in the manipulation, screening and killing of bacteria in a range of biotechnology processes. This kit specifically teaches the basic principles of antibiotics, bacterial resistance and susceptibility. Students learn and understand the use of antibiotic resistance in screening for infectious diseases. Utilising a bacterial strain, students learn the effects of different antibiotics and visualise bacterial sensitivity and resistance to the supplied antibiotics. This method involves the use of filter paper discs impregnated with a specified concentration of antibiotics on the surface of an agar plate containing microbial cells. This kit will enable students to analyse the inhibitory effects of different antibiotics on selected bacterial cells and then determine which antibiotic is the most suitable to treat a bacterial infection.

Supplier: DIDALAB

Triangular, cast aluminium optical bench

Supplier: DIDALAB

High quality professional gas laser, Class II